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  1. Article ; Online: The Clinical Significance and the Potential Regulatory Mechanism of the LncRNA OIP5-AS1 in Paediatric Severe Community-Acquired Pneumonia Blood Through the MiR-150-5p/PDCD4 Axis.

    Lu, Juan / Ren, Qingguo / Qi, Weiwei / Yang, Ning / He, Yuanyuan

    Immunological investigations

    2024  , Page(s) 1–18

    Abstract: Background: This study aimed to elucidate the clinical significance and regulatory mechanism of the long non-coding RNA OIP5-AS1 in severe community-acquired pneumonia (SCAP) among paediatric patients.: Methods: qRT-PCR was used to assess the mRNA ... ...

    Abstract Background: This study aimed to elucidate the clinical significance and regulatory mechanism of the long non-coding RNA OIP5-AS1 in severe community-acquired pneumonia (SCAP) among paediatric patients.
    Methods: qRT-PCR was used to assess the mRNA levels of OIP5-AS1. ROC curve analysis was used to assess the diagnostic significance of OIP5-AS1. Short-term prognostic significance was evaluated through Kaplan-Meier survival. An in vitro cell model was developed using LPS-induced MRC-5 cells. CCK-8, flow cytometry, and ELISA were conducted to measure cell viability, apoptosis, and inflammatory factor levels. The association between miR-150-5p and PDCD4 was confirmed through DLR assays.
    Results: Elevated OIP5-AS1 were observed in paediatric patients with SCAP, which enabled effective differentiation from healthy individuals. High expression of OIP5-AS1 correlated with reduced survival rates. OIP5-AS1 knockdown attenuated cell viability suppression and the promotion of apoptosis and inflammatory factors induced by LPS. However, this attenuation was reversed by reduced levels of miR-150-5p. miR-150-5p was identified as a target of PDCD4 and OIP5-AS1.
    Conclusion: Increased OIP5-AS1 levels show potential as a valuable diagnostic and prognostic biomarker for paediatric patients with SCAP. This study illustrates its role in regulating cell viability, apoptosis, and the inflammatory response via the miR-150-5p/PDCD4 axis, acting as a ceRNA.
    Language English
    Publishing date 2024-01-31
    Publishing country England
    Document type Journal Article
    ZDB-ID 632565-8
    ISSN 1532-4311 ; 0882-0139
    ISSN (online) 1532-4311
    ISSN 0882-0139
    DOI 10.1080/08820139.2024.2309557
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    Zs.A 988: Show issues Location:
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  2. Article ; Online: Carbon dot and silver nanoparticle-based fluorescent probe for the determination of sulfite and bisulfite via inner-filter effect and competitive redox reactions.

    He, Yuanyuan / Wang, Yongbo / Wang, Shaojie

    Mikrochimica acta

    2023  Volume 190, Issue 5, Page(s) 190

    Abstract: A new sensitive fluorescent probe (CDs-AgNP/ ... ...

    Abstract A new sensitive fluorescent probe (CDs-AgNP/H
    Language English
    Publishing date 2023-04-24
    Publishing country Austria
    Document type Journal Article
    ZDB-ID 89-9
    ISSN 1436-5073 ; 0026-3672
    ISSN (online) 1436-5073
    ISSN 0026-3672
    DOI 10.1007/s00604-023-05782-3
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  3. Article ; Online: Cold atmospheric plasma stabilizes mismatch repair for effective, uniform treatment of diverse colorectal cancer cell types.

    He, Yuanyuan / Lu, Fu / Jiang, Chenmin / Gong, Fanwu / Wu, Zhengwei / Ostrikov, Kostya

    Scientific reports

    2024  Volume 14, Issue 1, Page(s) 3599

    Abstract: Mismatch Repair (MMR) mechanisms play a pivotal role in rectifying DNA replication errors and maintaining the stability of DNA microsatellite structure. Colorectal cancer (CRC) can be characterized into microsatellite stability (MSS) and microsatellite ... ...

    Abstract Mismatch Repair (MMR) mechanisms play a pivotal role in rectifying DNA replication errors and maintaining the stability of DNA microsatellite structure. Colorectal cancer (CRC) can be characterized into microsatellite stability (MSS) and microsatellite instability (MSI) subtypes based on the functionality of MMR. MSI CRC notably exhibits enhanced chemotherapy resistance, attributable to diminished MMR-related protein expression. Cold atmospheric plasma (CAP) has emerged as a promising treatment modality, demonstrating efficacy in inducing apoptosis in various cancer cells. However, the therapeutic impact of CAP on MSI colorectal cancer, and the underlying mechanisms remain elusive. In this study, we investigated the effects of CAP on MSI (MC38, HCT116, and LOVO) and MSS (CT26 and HT29) CRC cell lines. We are probing into the products of CAP treatment. Our findings indicate that CAP treatment induces comparable effects on apoptosis, reactive oxygen species (ROS), and reactive nitrogen species (RNS), as well as the expression of apoptosis-related proteins in both MSI and MSS cells. Mechanistically, CAP treatment led to an elevation in the expression of mismatch repair proteins (MLH1 and MSH2), particularly in MSI cells, which notably have been proven to facilitate the activation of apoptosis-related proteins. Collectively, our study reveals that CAP enhances apoptotic signaling and induces apoptosis in MSI colorectal cancer cells by upregulating the expression of MMR-related proteins, thereby reinforcing MMR stabilization.
    MeSH term(s) Humans ; DNA Mismatch Repair ; Adaptor Proteins, Signal Transducing/genetics ; MutS Homolog 2 Protein/genetics ; Microsatellite Instability ; Microsatellite Repeats ; Colorectal Neoplasms/genetics ; Colorectal Neoplasms/drug therapy
    Chemical Substances Adaptor Proteins, Signal Transducing ; MutS Homolog 2 Protein (EC 3.6.1.3)
    Language English
    Publishing date 2024-02-13
    Publishing country England
    Document type Journal Article
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-024-54020-0
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  4. Article ; Online: Antibody inhibition of influenza A virus assembly and release.

    He, Yuanyuan / Guo, Zijian / Subiaur, Sofie / Benegal, Ananya / Vahey, Michael D

    Journal of virology

    2024  Volume 98, Issue 2, Page(s) e0139823

    Abstract: Antibodies are frontline defenders against influenza virus infection, providing protection through multiple complementary mechanisms. Although a subset of monoclonal antibodies (mAbs) has been shown to restrict replication at the level of virus assembly ... ...

    Abstract Antibodies are frontline defenders against influenza virus infection, providing protection through multiple complementary mechanisms. Although a subset of monoclonal antibodies (mAbs) has been shown to restrict replication at the level of virus assembly and release, it remains unclear how potent and pervasive this mechanism of protection is, due in part to the challenge of separating this effect from other aspects of antibody function. To address this question, we developed imaging-based assays to determine how effectively a broad range of mAbs against the IAV surface proteins can specifically restrict viral egress. We find that classically neutralizing antibodies against hemagglutinin are broadly multifunctional, inhibiting virus assembly and release at concentrations 1-20-fold higher than the concentrations at which they inhibit viral entry. These antibodies are also capable of altering the morphological features of shed virions, reducing the proportion of filamentous particles. We find that antibodies against neuraminidase and M2 also restrict viral egress and that inhibition by anti-neuraminidase mAbs is only partly attributable to a loss in enzymatic activity. In all cases, antigen crosslinking-either on the surface of the infected cell, between the viral and cell membrane, or both-plays a critical role in inhibition, and we are able to distinguish between these modes experimentally and through a structure-based computational model. Together, these results provide a framework for dissecting antibody multifunctionality that could help guide the development of improved therapeutic antibodies or vaccines and that can be extended to other viral families and antibody isotypes.IMPORTANCEAntibodies against influenza A virus provide multifaceted protection against infection. Although sensitive and quantitative assays are widely used to measure inhibition of viral attachment and entry, the ability of diverse antibodies to inhibit viral egress is less clear. We address this challenge by developing an imaging-based approach to measure antibody inhibition of virus release across a panel of monoclonal antibodies targeting the influenza A virus surface proteins. Using this approach, we find that inhibition of viral egress is common and can have similar potency to the ability of an antibody to inhibit viral entry. Insights into this understudied aspect of antibody function may help guide the development of improved countermeasures.
    MeSH term(s) Humans ; Antibodies, Monoclonal/administration & dosage ; Antibodies, Neutralizing/administration & dosage ; Antibodies, Viral ; Hemagglutinin Glycoproteins, Influenza Virus ; Influenza A virus/drug effects ; Influenza Vaccines ; Influenza, Human/drug therapy ; Influenza, Human/virology ; Membrane Proteins ; Neuraminidase/metabolism ; Virus Assembly/drug effects
    Chemical Substances Antibodies, Monoclonal ; Antibodies, Neutralizing ; Antibodies, Viral ; Hemagglutinin Glycoproteins, Influenza Virus ; Influenza Vaccines ; Membrane Proteins ; Neuraminidase (EC 3.2.1.18)
    Language English
    Publishing date 2024-01-05
    Publishing country United States
    Document type Journal Article
    ZDB-ID 80174-4
    ISSN 1098-5514 ; 0022-538X
    ISSN (online) 1098-5514
    ISSN 0022-538X
    DOI 10.1128/jvi.01398-23
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    Zs.A 609: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
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    Jg. 1995 - 2021: Lesesall (1.OG)
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  5. Article ; Online: Heterogeneous Porous Synergistic Photocatalysts for Organic Transformations.

    Zhu, Yuan-Yuan / He, Yuan-Yuan / Li, Yan-Xiang / Liu, Chun-Hua / Lin, Wenbin

    Chemistry (Weinheim an der Bergstrasse, Germany)

    2024  , Page(s) e202400842

    Abstract: Recent interest has surged in using heterogeneous carriers to boost synergistic photocatalysis for organic transformations. Heterogeneous catalysts not only facilitate synergistic enhancement of distinct catalytic centers compared to their homogeneous ... ...

    Abstract Recent interest has surged in using heterogeneous carriers to boost synergistic photocatalysis for organic transformations. Heterogeneous catalysts not only facilitate synergistic enhancement of distinct catalytic centers compared to their homogeneous counterparts, but also allow for the easy recovery and reuse of catalysts. This mini-review summarizes recent advancements in developing heterogeneous carriers, including metal-organic frameworks, covalent-organic frameworks, porous organic polymers, and others, for synergistic catalytic reactions. The advantages of porous materials in heterogeneous catalysis originate from their ability to provide a high surface area, facilitate enhanced mass transport, offer a tunable chemical structure, ensure the stability of active species, and enable easy recovery and reuse of catalysts. Both photosensitizers and catalysts can be intricately incorporated into suitable porous carriers to create heterogeneous dual photocatalysts for organic transformations. Notably, experimental evidence from reported cases has shown that the catalytic efficacy of heterogeneous catalysts often surpasses that of their homogeneous analogues. This enhanced performance is attributed to the proximity and confinement effects provided by the porous nature of the carriers. It is expected that porous carriers will provide a versatile platform for integrating diverse catalysts, thus exhibiting superior performance across a range of organic transformations and appealing prospect for industrial applications.
    Language English
    Publishing date 2024-05-01
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 1478547-X
    ISSN 1521-3765 ; 0947-6539
    ISSN (online) 1521-3765
    ISSN 0947-6539
    DOI 10.1002/chem.202400842
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  6. Article ; Online: Generating bioactive and antiseptic interfaces with nano-silver hydroxyapatite-based coatings by pulsed electrochemical deposition for long-term efficient cervical soft tissue sealing.

    Chen, Jie / Gai, Kuo / He, Yuanyuan / Xu, Yuchan / Guo, Weihua

    Journal of materials chemistry. B

    2023  Volume 11, Issue 2, Page(s) 345–358

    Abstract: Infections related to osseointegrated implants have sparked the interest in studying titanium modification for long-term effective soft tissue sealing. Constructing a silver (Ag)-hydroxyapatite (HA) coating is regarded as an effective strategy for ... ...

    Abstract Infections related to osseointegrated implants have sparked the interest in studying titanium modification for long-term effective soft tissue sealing. Constructing a silver (Ag)-hydroxyapatite (HA) coating is regarded as an effective strategy for integrating antibiosis with osteanagenesis; however, the outcome for long-term cervical soft tissue sealing
    MeSH term(s) Dogs ; Animals ; Durapatite/pharmacology ; Durapatite/chemistry ; Osteogenesis ; Coated Materials, Biocompatible/pharmacology ; Coated Materials, Biocompatible/chemistry ; Anti-Infective Agents, Local/pharmacology ; Anti-Bacterial Agents/pharmacology ; Anti-Bacterial Agents/chemistry
    Chemical Substances Durapatite (91D9GV0Z28) ; Coated Materials, Biocompatible ; Anti-Infective Agents, Local ; Anti-Bacterial Agents ; colloidal silver
    Language English
    Publishing date 2023-01-04
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2702241-9
    ISSN 2050-7518 ; 2050-750X
    ISSN (online) 2050-7518
    ISSN 2050-750X
    DOI 10.1039/d2tb02098j
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  7. Article ; Online: Risk factors for readmission for hyperbilirubinemia in neonates with ABO hemolytic disease: a single-center retrospective cohort study.

    Xu, Chuncai / Bao, Yingying / He, Yuanyuan / Wu, Mingyuan / Zhu, Jiajun

    The journal of maternal-fetal & neonatal medicine : the official journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians

    2023  Volume 36, Issue 2, Page(s) 2238106

    Abstract: Objective: ABO hemolytic disease of the newborn (ABO-HDN) is a major risk factor for severe hyperbilirubinemia, a common readmission reason for newborns. In this study, we aimed to assess the risk factors for readmission associated with ... ...

    Abstract Objective: ABO hemolytic disease of the newborn (ABO-HDN) is a major risk factor for severe hyperbilirubinemia, a common readmission reason for newborns. In this study, we aimed to assess the risk factors for readmission associated with hyperbilirubinemia in neonates with ABO-HDN.
    Methods: A retrospective cohort study was conducted including newborns with gestational age ≥35 weeks and ABO-HDN in 2018. Among 291 newborns, 36 were readmitted for hyperbilirubinemia and defined as the readmission group. The remaining 255 cases were used as a control group. We then performed between-group comparisons of clinical conditions associated with hyperbilirubinemia. Logistic regression was used to select risk predictors of readmission associated with hyperbilirubinemia due to ABO-HDN.
    Results: Baseline characteristics were similar between both groups (
    Conclusions: Earlier age of phototherapy initiation, higher TSB levels at the time of initiating phototherapy and rebound hyperbilirubinemia after the first phototherapy treatment may increase the risk of readmission for hyperbilirubinemia in neonates with ABO-HDN. These factors should be considered in discharge planning and follow-up for newborns with ABO-HDN associated hyperbilirubinemia.
    MeSH term(s) Female ; Infant, Newborn ; Humans ; Infant ; Retrospective Studies ; Patient Readmission ; Bilirubin ; Hyperbilirubinemia/therapy ; Erythroblastosis, Fetal/therapy ; Risk Factors ; Phototherapy ; Hyperbilirubinemia, Neonatal/therapy ; ABO Blood-Group System
    Chemical Substances Bilirubin (RFM9X3LJ49) ; ABO Blood-Group System
    Language English
    Publishing date 2023-07-24
    Publishing country England
    Document type Journal Article
    ZDB-ID 2077261-0
    ISSN 1476-4954 ; 1057-0802 ; 1476-7058
    ISSN (online) 1476-4954
    ISSN 1057-0802 ; 1476-7058
    DOI 10.1080/14767058.2023.2238106
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    In stock of ZB MED Cologne/Königswinter

    Zs.A 3510: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
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    Jg. 1995 - 2021: Lesesall (2.OG)
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  8. Article ; Online: Plasma-microbubble treatment and sustainable agriculture application of diclofenac-contaminated wastewater

    Liu, Qi / Ouyang, Wenchong / Yang, Xusheng / He, Yuanyuan / Wu, Zhengwei / Ostrikov, Kostya (Ken)

    Elsevier Ltd Chemosphere. 2023 Sept., v. 334 p.138998-

    2023  

    Abstract: The demand for efficient wastewater treatment is becoming increasingly urgent due to the rising threat of pharmaceutical residues in water. As a sustainable advanced oxidation process, cold plasma technology is a promising approach for water treatment. ... ...

    Abstract The demand for efficient wastewater treatment is becoming increasingly urgent due to the rising threat of pharmaceutical residues in water. As a sustainable advanced oxidation process, cold plasma technology is a promising approach for water treatment. However, the adoption of the technology encounters several challenges, including the low treatment efficiency and the potentially unknown environmental impact. Here, microbubble generation was integrated with cold plasma system to enhance treatment of wastewater contaminated with diclofenac (DCF). The degradation efficiency depended on the discharge voltage, gas flow, initial concentration, and pH value. The best degradation efficiency was 90.9% after 45 min plasma-bubble treatment under the optimum process parameters. The hybrid plasma-bubble system exhibited strongly synergistic performance heralded by up to seven-times higher DCF removal rates than the two systems operated separately. The plasma-bubble treatment remains effective even after addition of SO₄²⁻, Cl⁻, CO₃²⁻, HCO₃⁻, and humic acid (HA) as interfering background substances. The contributions of •O₂⁻, O₃, •OH, and H₂O₂ reactive species to the DCF degradation process were specified. The synergistic mechanisms for DCF degradation were deduced through the analysis of the degradation intermediates. Further, the plasma-bubble treated water was proven safe and effective to stimulate seed germination and plant growth for sustainable agriculture applications. Overall, these findings provide new insights and a feasible approach with a highly synergistic removal effect for the plasma-enhanced microbubble wastewater treatment, without generating secondary contaminants.
    Keywords cold ; diclofenac ; electric potential difference ; environmental impact ; humic acids ; microbubbles ; oxidation ; pH ; plant growth ; seed germination ; sustainable agriculture ; wastewater ; wastewater treatment ; Cold plasma ; Diclofenac degradation ; Synergistic effect
    Language English
    Dates of publication 2023-09
    Publishing place Elsevier Ltd
    Document type Article ; Online
    ZDB-ID 120089-6
    ISSN 1879-1298 ; 0045-6535 ; 0366-7111
    ISSN (online) 1879-1298
    ISSN 0045-6535 ; 0366-7111
    DOI 10.1016/j.chemosphere.2023.138998
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    Zs.A 2540: Show issues Location:
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  9. Article: Performance and mechanisms for V(v) bio-reduction by straw: key influencing factors

    Hao, Liting / He, Yuanyuan / Shi, Chen / Hao, Xiaodi

    RSC advances. 2021 Aug. 10, v. 11, no. 44

    2021  

    Abstract: A high concentration of vanadium [V(v)] in groundwater is extremely harmful for humans. Weak movability and low toxicity after microbial V(v) reduction have attracted remarkable attention, especially for using solid carbon sources. However, the ... ...

    Abstract A high concentration of vanadium [V(v)] in groundwater is extremely harmful for humans. Weak movability and low toxicity after microbial V(v) reduction have attracted remarkable attention, especially for using solid carbon sources. However, the influencing factors remain unclear. In this study, the initial V(v) concentration, inocula amount and straw dosage were examined to ascertain the mechanisms behind them. Increasing the initial V(v) concentration led to the decrease of the V(v) removal efficiency, which was also positively correlated with the straw dosage within a certain range. The initial sludge amount was not a main factor affecting microbial V(v) removal in this study. With the initial amount of 10 mg L⁻¹ V(v), 25 mL initial inocula and 5 g straw, 88.2% of V(v) was removed. According to the dissolved organic matter (DOM) analysis results, microbial activity prevailed in groups with higher V(v) removal efficiency, indicating that the V(v) bio-reduction was attributed to the microbial activity, which was considered a major factor. Functional species as unclassified_f_Enterobacteriaceae presumably contributed to the V(v) bioreduction, with upregulated ABC transporter genes and enzymes.
    Keywords ABC transporters ; carbon ; dissolved organic matter ; groundwater ; inoculum ; microbial activity ; sludge ; straw ; toxicity ; vanadium
    Language English
    Dates of publication 2021-0810
    Size p. 27246-27256.
    Publishing place The Royal Society of Chemistry
    Document type Article
    ISSN 2046-2069
    DOI 10.1039/d1ra03201a
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  10. Article: Biologically removing vanadium(V) from groundwater by agricultural biomass

    Hao, Liting / He, Yuanyuan / Shi, Chen / Hao, Xiaodi

    Journal of environmental management. 2021 Oct. 15, v. 296

    2021  

    Abstract: Vanadium (V) in groundwater can pose a serious threat on both environment and health. Agricultural biomass contains solid carbon source (SCS) and could be attractive for biologically removing V(V). For this purpose, cypress sawdust, corn cob and wheat ... ...

    Abstract Vanadium (V) in groundwater can pose a serious threat on both environment and health. Agricultural biomass contains solid carbon source (SCS) and could be attractive for biologically removing V(V). For this purpose, cypress sawdust, corn cob and wheat straw were selected as SCSs to remove vanadate (NaVO₃). The experiments demonstrated a high efficiency of V(V) up to 98.6%, and the anaerobically biological reduction of V(V) to V(IV) by wheat straw was identified to be the best SCS by the spectrum analysis of XRD and FTIR. Along with increasing the fragment size of wheat straw, the V(V)-removal efficiency decreased, and the fragment size down to 1–3 mm was confirmed to have a significant bio-removal performance on V(V). Based on the analysis of 16s rRNA sequencing, the microbial abundance and diversity increased in the suspension liquid in the end, indicating that the microbial community could tolerate and/or detoxify V(V), besides degrading lignocellulosic materials.
    Keywords biomass ; carbon ; corn cobs ; environmental management ; groundwater ; lignocellulose ; liquids ; microbial communities ; sawdust ; vanadium ; wheat straw
    Language English
    Dates of publication 2021-1015
    Publishing place Elsevier Ltd
    Document type Article
    ZDB-ID 184882-3
    ISSN 1095-8630 ; 0301-4797
    ISSN (online) 1095-8630
    ISSN 0301-4797
    DOI 10.1016/j.jenvman.2021.113244
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    Z 7556: Show issues

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