Article ; Online: A high efficiency precision genome editing method with CRISPR in iPSCs.
2024 Volume 14, Issue 1, Page(s) 9933
Abstract: The use of genetic engineering to generate point mutations in induced pluripotent stem cells (iPSCs) is essential for studying a specific genetic effect in an isogenic background. We demonstrate that a combination of p53 inhibition and pro-survival small ...
Abstract | The use of genetic engineering to generate point mutations in induced pluripotent stem cells (iPSCs) is essential for studying a specific genetic effect in an isogenic background. We demonstrate that a combination of p53 inhibition and pro-survival small molecules achieves a homologous recombination rate higher than 90% using Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) in human iPSCs. Our protocol reduces the effort and time required to create isogenic lines. |
---|---|
MeSH term(s) | Induced Pluripotent Stem Cells/metabolism ; Induced Pluripotent Stem Cells/cytology ; Humans ; Gene Editing/methods ; CRISPR-Cas Systems ; Tumor Suppressor Protein p53/genetics ; Clustered Regularly Interspaced Short Palindromic Repeats/genetics ; Homologous Recombination |
Chemical Substances | Tumor Suppressor Protein p53 |
Language | English |
Publishing date | 2024-04-30 |
Publishing country | England |
Document type | Journal Article ; Research Support, Non-U.S. Gov't |
ZDB-ID | 2615211-3 |
ISSN | 2045-2322 ; 2045-2322 |
ISSN (online) | 2045-2322 |
ISSN | 2045-2322 |
DOI | 10.1038/s41598-024-60766-4 |
Database | MEDical Literature Analysis and Retrieval System OnLINE |
More links
Kategorien
Order via subito
This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.