Article ; Online: Assessment of sample pooling for SARS-CoV-2 molecular testing for screening of asymptomatic persons in Tunisia.
Diagnostic microbiology and infectious disease
2020 Volume 98, Issue 3, Page(s) 115125
Abstract: ... of SARS-CoV-2. We found that a single positive specimen can still be detected in pools of up to 10 ... The aim of this study is to test a pooling approach for the RT-PCR test to detect low viral loads ... Each laboratory should conduct its own evaluation and validation of pooling protocols according to its specific ...
| Abstract | The aim of this study is to test a pooling approach for the RT-PCR test to detect low viral loads of SARS-CoV-2. We found that a single positive specimen can still be detected in pools of up to 10. Each laboratory should conduct its own evaluation and validation of pooling protocols according to its specific context. |
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| MeSH term(s) | Betacoronavirus/genetics ; COVID-19 ; COVID-19 Testing ; Clinical Laboratory Techniques/methods ; Coronavirus Infections/diagnosis ; Humans ; Mass Screening/methods ; Pandemics ; Pneumonia, Viral/diagnosis ; RNA, Viral/genetics ; Reverse Transcriptase Polymerase Chain Reaction/methods ; SARS-CoV-2 ; Specimen Handling ; Tunisia ; Viral Load/genetics |
| Chemical Substances | RNA, Viral |
| Keywords | covid19 |
| Language | English |
| Publishing date | 2020-07-05 |
| Publishing country | United States |
| Document type | Journal Article |
| ZDB-ID | 604920-5 |
| ISSN | 1879-0070 ; 0732-8893 |
| ISSN (online) | 1879-0070 |
| ISSN | 0732-8893 |
| DOI | 10.1016/j.diagmicrobio.2020.115125 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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