Article: Ancient conserved domain protein-1 binds copper and modifies its retention in cells.
2007 Volume 103, Issue 1, Page(s) 312–321
Abstract: ... response elements and the cellular expression of ACDP-1 alters cellular retention of copper. However, cellular ... The ancient conserved domain protein (ACDP) family are a recently identified group of homologous ... isothermal titration calorimetry we determined that ACDP-1 is a high affinity copper binding protein able ...
| Abstract | The ancient conserved domain protein (ACDP) family are a recently identified group of homologous mammalian proteins. Some family members have been suggested to have roles in the metabolism of metals. We investigated the capacity of ACDP-1 to bind metals. Using immobilised metal affinity chromatography and isothermal titration calorimetry we determined that ACDP-1 is a high affinity copper binding protein able to bind copper at nanomolar concentrations. In addition the promoter of ACDP-1 contains metal response elements and the cellular expression of ACDP-1 alters cellular retention of copper. However, cellular expression of ACDP-1 does not alter cellular resistance to the toxicity of copper or other metals. As our findings place the subcellular localisation of ACDP-1 in the cytoplasm it is possible that ACDP-1 represent a novel copper chaperone or storage protein. |
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| MeSH term(s) | Amino Acid Sequence ; Animals ; COS Cells ; Carrier Proteins/genetics ; Carrier Proteins/metabolism ; Cation Transport Proteins ; Cell Line ; Cercopithecus aethiops ; Cloning, Molecular ; Copper/metabolism ; Copper/toxicity ; Cyclins/genetics ; Cyclins/metabolism ; Cytoplasm/metabolism ; Metals, Heavy/toxicity ; Mice ; Molecular Sequence Data ; Sequence Homology, Amino Acid |
| Chemical Substances | ACDP-1 protein, mouse ; Carrier Proteins ; Cation Transport Proteins ; Cyclins ; Metals, Heavy ; Copper (789U1901C5) |
| Language | English |
| Publishing date | 2007-10 |
| Publishing country | England |
| Document type | Journal Article |
| ZDB-ID | 80158-6 |
| ISSN | 1471-4159 ; 0022-3042 ; 1474-1644 |
| ISSN (online) | 1471-4159 |
| ISSN | 0022-3042 ; 1474-1644 |
| DOI | 10.1111/j.1471-4159.2007.04751.x |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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