Article ; Online: Breathing fresh air into respiratory research with single-cell RNA sequencing.
European respiratory review : an official journal of the European Respiratory Society
2020 Volume 29, Issue 156
Abstract: ... cell RNA sequencing (scRNA-seq) provides a robust, unbiased survey of the transcriptome comparable ... to bulk RNA sequencing while preserving information on cellular heterogeneity. In just a few years ... the evolution of single-cell technologies with a focus on spatial and multi-omics approaches that promise ...
| Abstract | The complex cellular heterogeneity of the lung poses a unique challenge to researchers in the field. While the use of bulk RNA sequencing has become a ubiquitous technology in systems biology, the technique necessarily averages out individual contributions to the overall transcriptional landscape of a tissue. Single-cell RNA sequencing (scRNA-seq) provides a robust, unbiased survey of the transcriptome comparable to bulk RNA sequencing while preserving information on cellular heterogeneity. In just a few years since this technology was developed, scRNA-seq has already been adopted widely in respiratory research and has contributed to impressive advancements such as the discoveries of the pulmonary ionocyte and of a profibrotic macrophage population in pulmonary fibrosis. In this review, we discuss general technical considerations when considering the use of scRNA-seq and examine how leading investigators have applied the technology to gain novel insights into respiratory biology, from development to disease. In addition, we discuss the evolution of single-cell technologies with a focus on spatial and multi-omics approaches that promise to drive continued innovation in respiratory research. |
|||||
|---|---|---|---|---|---|---|
| MeSH term(s) | Computational Biology/methods ; Gene Expression Profiling ; Humans ; Lung Diseases/genetics ; Pulmonary Medicine/trends ; Sequence Analysis, RNA/methods ; Single-Cell Analysis/methods ; Transcriptome | |||||
| Keywords | covid19 | |||||
| Language | English | |||||
| Publishing date | 2020-07-03 | |||||
| Publishing country | England | |||||
| Document type | Journal Article ; Review | |||||
| ZDB-ID | 1077620-5 | |||||
| ISSN | 1600-0617 ; 0905-9180 | |||||
| ISSN (online) | 1600-0617 | |||||
| ISSN | 0905-9180 | |||||
| DOI | 10.1183/16000617.0060-2020 | |||||
| Shelf mark |
|
|||||
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
More links
Kategorien
In stock of ZB MED Cologne/Königswinter
| Zs.A 3265: Show issues | Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (2.OG) ab Jg. 2022: Lesesaal (EG) |
Order via subito
This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.