Article ; Online: Role of Reverse Transcriptase Polymerase Chain Reaction in Cornea Donors During the COVID-19 Pandemic.
2021 Volume 40, Issue 8, Page(s) 1044–1047
Abstract: ... of reverse transcriptase polymerase chain reaction (RT-PCR) of nasopharyngeal (NP) samples of cornea donors dying because of causes unrelated ... of these 11 donors had a Ct value of E gene less than 25.: Conclusions: NP samples of cornea donors dying ... adding the routine testing of NP samples of all cornea donors in the eye banking protocol in this ongoing ...
| Abstract | Purpose: The purpose of this study was to report the analysis of reverse transcriptase polymerase chain reaction (RT-PCR) of nasopharyngeal (NP) samples of cornea donors dying because of causes unrelated to severe acute respiratory coronavirus (SARS-CoV-2). Methods: A retrospective analysis of all cornea donors dying from causes other than SARS-CoV-2 between August 2020 and December 2020 was performed. Informed consent was obtained from the next of kin of the deceased for RT-PCR testing from NP swabs. Rapid antigen testing from all the deceased was performed before in situ cornea excision. In addition, NP samples in viral transport media for RT-PCR were also collected for SARS-CoV-2 analysis. Corneas were released from the eye bank only after a negative RT-PCR report. Result: One hundred eighteen corneas from 59 donors were obtained by the eye bank. Eleven donors (18.64%) were positive for SARS-CoV-2 on RT-PCR testing. Six of these 11 donors had a Ct value of E gene less than 25. Conclusions: NP samples of cornea donors dying due to causes other than coronavirus disease-19 were positive for SARS-CoV-2 on RT-PCR. This implicates that donors could be having asymptomatic/undetected coronavirus disease infection. We recommend adding the routine testing of NP samples of all cornea donors in the eye banking protocol in this ongoing SARS-CoV-2 pandemic. |
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| MeSH term(s) | Adolescent ; Adult ; Aged ; Aged, 80 and over ; Asymptomatic Infections/mortality ; COVID-19/epidemiology ; COVID-19 Nucleic Acid Testing ; Cornea ; Eye Banks/statistics & numerical data ; Humans ; Middle Aged ; Nasopharynx/virology ; RNA, Viral/genetics ; Retrospective Studies ; SARS-CoV-2/genetics ; Tissue Donors/supply & distribution ; Young Adult | |||||
| Chemical Substances | RNA, Viral | |||||
| Language | English | |||||
| Publishing date | 2021-04-28 | |||||
| Publishing country | United States | |||||
| Document type | Journal Article | |||||
| ZDB-ID | 604826-2 | |||||
| ISSN | 1536-4798 ; 0277-3740 | |||||
| ISSN (online) | 1536-4798 | |||||
| ISSN | 0277-3740 | |||||
| DOI | 10.1097/ICO.0000000000002742 | |||||
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| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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