Article: Use of chemical auxiliaries to control p450 enzymes for predictable oxidations at unactivated C-h bonds of substrates.
Advances in experimental medicine and biology
2015 Volume 851, Page(s) 209–228
Abstract: Cytochrome P450 enzymes (P450s) have the ability to oxidize unactivated C-H bonds of substrates ... their impressive attributes, the current use of P450s as biocatalysts is limited. While bacterial P450 enzymes ... On the other hand, mammalian P450s, especially the drug-metabolizing enzymes, display astonishing substrate ...
| Abstract | Cytochrome P450 enzymes (P450s) have the ability to oxidize unactivated C-H bonds of substrates with remarkable regio- and stereoselectivity. Comparable selectivity for chemical oxidizing agents is typically difficult to achieve. Hence, there is an interest in exploiting P450s as potential biocatalysts. Despite their impressive attributes, the current use of P450s as biocatalysts is limited. While bacterial P450 enzymes typically show higher activity, they tend to be highly selective for one or a few substrates. On the other hand, mammalian P450s, especially the drug-metabolizing enzymes, display astonishing substrate promiscuity. However, product prediction continues to be challenging. This review discusses the use of small molecules for controlling P450 substrate specificity and product selectivity. The focus will be on two approaches in the area: (1) the use of decoy molecules, and (2) the application of substrate engineering to control oxidation by the enzyme. |
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| MeSH term(s) | Animals ; Bacteria/enzymology ; Bacterial Proteins/chemistry ; Bacterial Proteins/metabolism ; Catalysis ; Cytochrome P-450 Enzyme System/chemistry ; Cytochrome P-450 Enzyme System/metabolism ; Humans ; Oxidation-Reduction ; Substrate Specificity |
| Chemical Substances | Bacterial Proteins ; Cytochrome P-450 Enzyme System (9035-51-2) |
| Language | English |
| Publishing date | 2015 |
| Publishing country | United States |
| Document type | Journal Article ; Research Support, Non-U.S. Gov't ; Review |
| ISSN | 2214-8019 ; 0065-2598 |
| ISSN (online) | 2214-8019 |
| ISSN | 0065-2598 |
| DOI | 10.1007/978-3-319-16009-2_8 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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