Article ; Online: Use of an Automated Nested Multiplex Respiratory Pathogen PCR Panel Postmortem in the Pediatric Forensic Setting.
2017 Volume 62, Issue 5, Page(s) 1223–1228
Abstract: ... however, no study has examined the use of automated, nested, multiplex polymerase chain reaction (ANM-PCR ... of ANM-PCR in detecting respiratory pathogens in the pediatric forensic setting. Respiratory samples ... Respiratory pathogens have been detected in forensic investigations using multiple techniques ...
| Abstract | Respiratory pathogens have been detected in forensic investigations using multiple techniques; however, no study has examined the use of automated, nested, multiplex polymerase chain reaction (ANM-PCR), commonly used in living patients, in the forensic setting. This retrospective study assessed the utility of ANM-PCR in detecting respiratory pathogens in the pediatric forensic setting. Respiratory samples from 35 cases were tested for up to 20 respiratory pathogens. 51.4% of these cases yielded a positive ANM-PCR result, 20% of which were considered the cause of or contributory to death. The most commonly detected pathogens were rhinovirus/enterovirus and respiratory syncytial virus, and these were the only pathogens determined to play a significant role in cause of death. The sampled sites and postmortem intervals tested did not affect the likelihood of a positive or negative test. ANM-PCR panels are effective, affordable, and rapid ancillary tools in evaluating cause of death in the forensic pediatric population. |
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| MeSH term(s) | Adenoviridae/genetics ; Adenoviridae/isolation & purification ; Child ; Child, Preschool ; Coronavirus/genetics ; Coronavirus/isolation & purification ; DNA, Viral/genetics ; Enterovirus/genetics ; Enterovirus/isolation & purification ; Female ; Forensic Pathology ; Humans ; Infant ; Infant, Newborn ; Male ; Multiplex Polymerase Chain Reaction/methods ; Respiratory Syncytial Virus, Human/genetics ; Respiratory Syncytial Virus, Human/isolation & purification ; Respiratory Tract Infections/virology ; Retrospective Studies ; Rhinovirus/genetics ; Rhinovirus/isolation & purification | |||||
| Chemical Substances | DNA, Viral | |||||
| Keywords | covid19 | |||||
| Language | English | |||||
| Publishing date | 2017-01-25 | |||||
| Publishing country | United States | |||||
| Document type | Journal Article | |||||
| ZDB-ID | 219216-0 | |||||
| ISSN | 1556-4029 ; 0022-1198 | |||||
| ISSN (online) | 1556-4029 | |||||
| ISSN | 0022-1198 | |||||
| DOI | 10.1111/1556-4029.13415 | |||||
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| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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