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Article: [Effects of collagen type ⅩⅦ α1 on epidermal stem cells in aging skin and the microRNA intervention mechanism].

Sun, J C / Sun, T J / Shen, Z A / Zhao, H Q / Liu, X Z / Zhang, Y J

Zhonghua shao shang yu chuang mian xiu fu za zhi

2022 Volume 38, Issue 9, Page(s) 839–848

Abstract: Objective: ...

Abstract	Objective:
MeSH term(s)	Adolescent ; Adult ; Aged ; Animals ; Autoantigens ; Humans ; Keratin-14 ; Male ; Mice ; Mice, Inbred C57BL ; MicroRNAs/genetics ; Non-Fibrillar Collagens/pharmacology ; Polyesters ; RNA, Messenger ; Skin Aging ; Stem Cells ; Young Adult ; Collagen Type XVII
Chemical Substances	Autoantigens ; Keratin-14 ; MIRN329 microRNA, human ; MIRN590 microRNA, human ; MicroRNAs ; Non-Fibrillar Collagens ; Polyesters ; RNA, Messenger
Language	Chinese
Publishing date	2022-12-15
Publishing country	China
Document type	Journal Article
ISSN	2097-1109
ISSN	2097-1109
DOI	10.3760/cma.j.cn501120-20210829-00293
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Article ; Online: One-Step Simple Isolation Method to Obtain Both Epidermal and Dermal Stem Cells from Human Skin Specimen.

Qian, Hua / Leng, Xue / Wen, Jie / Zhou, Qian / Xu, Xin / Wu, Xunwei

Methods in molecular biology (Clifton, N.J.)

2018 Volume 1879, Page(s) 139–148

Abstract: ... following injury. The efficient procurement of high quantity and quality of skin stem cells is important ... consuming and can efficiently obtain the high quality of skin stem cells and can maintain the stem cell ... obtain both epidermal and dermal cell population from human skin specimen, based on the different ...

Abstract	Stem cells play a crucial role in maintaining and repairing tissues during homeostasis and following injury. The efficient procurement of high quantity and quality of skin stem cells is important for both laboratory studies and clinical applications. Here, we describe a one-step isolation procedure to efficiently obtain both epidermal and dermal cell population from human skin specimen, based on the different influence of the Rho kinase inhibitor Y27632 on the growth of epidermal and dermal cells during the initial culture. Compared with the conventional methods, our protocol shows that it is simpler and less time consuming and can efficiently obtain the high quality of skin stem cells and can maintain the stem cell features after culture expansion.
MeSH term(s)	Amides/pharmacology ; Cell Proliferation/drug effects ; Cell Proliferation/physiology ; Cells, Cultured ; Epidermal Cells/cytology ; Epidermal Cells/drug effects ; Humans ; Pyridines/pharmacology ; Skin/cytology ; Skin/drug effects ; Stem Cells/cytology ; Stem Cells/drug effects ; Wound Healing/physiology
Chemical Substances	Amides ; Pyridines ; Y 27632 (138381-45-0)
Language	English
Publishing date	2018-03-26
Publishing country	United States
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ISSN	1940-6029
ISSN (online)	1940-6029
DOI	10.1007/7651_2018_117
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Article: Epidermal stem cells of the skin.

Blanpain, Cédric / Fuchs, Elaine

Annual review of cell and developmental biology

2006 Volume 22, Page(s) 339–373

Abstract: ... a perpetual cycle of growth and degeneration. Stem cells (SCs) residing in the epidermis and hair follicle ... The skin constantly renews itself throughout adult life, and the hair follicle undergoes ... ensure the maintenance of adult skin homeostasis and hair regeneration, but they also participate ...

Abstract	The skin constantly renews itself throughout adult life, and the hair follicle undergoes a perpetual cycle of growth and degeneration. Stem cells (SCs) residing in the epidermis and hair follicle ensure the maintenance of adult skin homeostasis and hair regeneration, but they also participate in the repair of the epidermis after injuries. We summarize here the current knowledge of epidermal SCs of the adult skin. We discuss their fundamental characteristics, the methods recently designed to isolate these cells, the genes preferentially expressed in the multipotent SC niche, and the signaling pathways involved in SC niche formation, SC maintenance, and activation. Finally, we speculate on how the deregulation of these pathways may lead to cancer formation.
MeSH term(s)	Animals ; Cell Lineage ; Epidermis/cytology ; Epithelium/metabolism ; Hair Follicle/cytology ; Hair Follicle/embryology ; Humans ; Morphogenesis ; Stem Cells/cytology
Language	English
Publishing date	2006
Publishing country	United States
Document type	Journal Article ; Review
ZDB-ID	1293750-2
ISSN	1530-8995 ; 1081-0706
ISSN (online)	1530-8995
ISSN	1081-0706
DOI	10.1146/annurev.cellbio.22.010305.104357
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Article ; Online: Development of pluripotent stem cell-derived epidermal organoids that generate effective extracellular vesicles in skin regeneration.

Kwak, Sojung / Song, Cho Lok / Lee, Jinhyuk / Kim, Sungyeon / Nam, Seungyoon / Park, Young-Jun / Lee, Jungwoon

Biomaterials

2024 Volume 307, Page(s) 122522

Abstract: ... into other skin compartment lineages, such as hair follicle stem cells. By closely recapitulating the epidermal ... we report the development of epidermal organoids (EpiOs) generated from induced pluripotent stem cells ... including wound healing and skin regeneration. These cellular models are mostly derived from primary cells ...

Abstract	Cellular skin substitutes such as epidermal constructs have been developed for various applications, including wound healing and skin regeneration. These cellular models are mostly derived from primary cells such as keratinocytes and fibroblasts in a two-dimensional (2D) state, and further development of three-dimensional (3D) cultured organoids is needed to provide insight into the in vivo epidermal phenotype and physiology. Here, we report the development of epidermal organoids (EpiOs) generated from induced pluripotent stem cells (iPSCs) as a novel epidermal construct and its application as a source of secreted biomolecules recovered by extracellular vesicles (EVs) that can be utilized for cell-free therapy of regenerative medicine. Differentiated iPSC-derived epidermal organoids (iEpiOs) are easily cultured and expanded through multiple organoid passages, while retaining molecular and functional features similar to in vivo epidermis. These mature iEpiOs contain epidermal stem cell populations and retain the ability to further differentiate into other skin compartment lineages, such as hair follicle stem cells. By closely recapitulating the epidermal structure, iEpiOs are expected to provide a more relevant microenvironment to influence cellular processes and therapeutic response. Indeed, iEpiOs can generate high-performance EVs containing high levels of the angiogenic growth factor VEGF and miRNAs predicted to regulate cellular processes such as proliferation, migration, differentiation, and angiogenesis. These EVs contribute to target cell proliferation, migration, and angiogenesis, providing a promising therapeutic tool for in vivo wound healing. Overall, the newly developed iEpiOs strategy as an organoid-based approach provides a powerful model for studying basic and translational skin research and may also lead to future therapeutic applications using iEpiOs-secreted EVs.
MeSH term(s)	Epidermis ; Pluripotent Stem Cells ; Cell Differentiation ; Organoids ; Extracellular Vesicles ; Regeneration
Language	English
Publishing date	2024-02-23
Publishing country	Netherlands
Document type	Journal Article
ZDB-ID	603079-8
ISSN	1878-5905 ; 0142-9612
ISSN (online)	1878-5905
ISSN	0142-9612
DOI	10.1016/j.biomaterials.2024.122522
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Article: Adipose mesenchymal stem cell-derived exosomes promote skin wound healing in diabetic mice by regulating epidermal autophagy.

Ren, Haiyue / Su, Peng / Zhao, Feng / Zhang, Qiqi / Huang, Xing / He, Cai / Wu, Quan / Wang, Zitong / Ma, Jiajie / Wang, Zhe

Burns & trauma

2024 Volume 12, Page(s) tkae001

Abstract: ... autophagy in skin epithelial cells, thereby providing a new theoretical basis for the treatment and repair ... the biological function of epidermal cells under high-glucose conditions and partially weakened the healing ... effect of ADSC-Exos. Using a diabetes wound model, we found that ADSC-Exos promoted skin wound healing ...

Abstract	Background: Adipose mesenchymal stem cell-derived exosomes (ADSC-Exos) have great potential in the field of tissue repair and regenerative medicine, particularly in cases of refractory diabetic wounds. Interestingly, autophagy plays a role in wound healing, and recent research has demonstrated that exosomes are closely associated with intracellular autophagy in biogenesis and molecular signaling mechanisms. Therefore, this study aimed to investigate whether ADSC-Exos promote the repair of diabetic wounds by regulating autophagy to provide a new method and theoretical basis for the treatment of diabetic wounds. Methods: Western blot analysis and autophagy double-labelled adenovirus were used to monitor changes in autophagy flow in human immortalized keratinocyte cell line (HaCaT) cells. ADSC-Exos were generated from ADSC supernatants via ultracentrifugation. The effectiveness of ADSC-Exos on HaCaT cells was assessed using a live-cell imaging system, cell counting kit-8 and cell scratch assays. The cells were treated with the autophagy inhibitor bafilomycin A1 to evaluate the effects of autophagy on cell function. The recovery of diabetic wounds after ADSC-Exo treatment was determined by calculating the healing rates and performing histological analysis. High-throughput transcriptome sequencing was used to analyze changes in mRNA expression after the treatment of HaCaT cells with ADSC-Exos. Results: ADSC-Exos activated autophagy in HaCaT cells, which was inhibited by high glucose levels, and potentiated their cellular functions. Moreover, ADSC-Exos in combination with the autophagy inhibitor bafilomycin A1 showed that autophagy defects further impaired the biological function of epidermal cells under high-glucose conditions and partially weakened the healing effect of ADSC-Exos. Using a diabetes wound model, we found that ADSC-Exos promoted skin wound healing in diabetic mice, as evidenced by increased epidermal autophagy and rapid re-epithelialization. Finally, sequencing results showed that increased expression of autophagy-related genes nicotinamide phosphoribosyltransferase ( Conclusions: This study elucidated the molecular mechanism through which ADCS-Exos regulate autophagy in skin epithelial cells, thereby providing a new theoretical basis for the treatment and repair of skin epithelial damage by ADSC-Exos.
Language	English
Publishing date	2024-02-29
Publishing country	England
Document type	Journal Article
ZDB-ID	2775996-9
ISSN	2321-3876 ; 2321-3868
ISSN (online)	2321-3876
ISSN	2321-3868
DOI	10.1093/burnst/tkae001
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Article ; Online: Epidermal homeostasis: a balancing act of stem cells in the skin.

Blanpain, Cédric / Fuchs, Elaine

Nature reviews. Molecular cell biology

2009 Volume 10, Issue 3, Page(s) 207–217

Abstract: ... Different resident skin stem cell pools contribute to the maintenance and repair of the various epidermal ... homeostasis. Stem cells in the epidermis have a crucial role in maintaining tissue homeostasis by providing ... The skin epidermis and its array of appendages undergo ongoing renewal by a process called ...

Abstract	The skin epidermis and its array of appendages undergo ongoing renewal by a process called homeostasis. Stem cells in the epidermis have a crucial role in maintaining tissue homeostasis by providing new cells to replace those that are constantly lost during tissue turnover or following injury. Different resident skin stem cell pools contribute to the maintenance and repair of the various epidermal tissues of the skin, including interfollicular epidermis, hair follicles and sebaceous glands. Interestingly, the basic mechanisms and signalling pathways that orchestrate epithelial morphogenesis in the skin are reused during adult life to regulate skin homeostasis.
MeSH term(s)	Animals ; Cells, Cultured ; Epidermal Cells ; Epithelial Cells/cytology ; Homeostasis ; Humans ; Models, Biological ; Skin/cytology ; Stem Cells/physiology
Language	English
Publishing date	2009-02-11
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't ; Review
ZDB-ID	2031313-5
ISSN	1471-0080 ; 1471-0072
ISSN (online)	1471-0080
ISSN	1471-0072
DOI	10.1038/nrm2636
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Article ; Online: Single-stage transplantation combined with epidermal stem cells promotes the survival of tissue-engineered skin by inducing early angiogenesis.

Wang, Zhiyong / Xu, Hailin / Yang, Hao / Zhang, Yi / Wang, Xiaoyan / Wang, Peng / Xu, Zhongye / Lv, Dongming / Rong, Yanchao / Dong, Yunxian / Tang, Bing / Hu, Zhicheng / Deng, Wuguo / Zhu, Jiayuan

Stem cell research & therapy

2023 Volume 14, Issue 1, Page(s) 51

Abstract: ... defects via a single-stage procedure. Epidermal stem cells (EpSCs) have been found to promote angiogenesis ... Background: The composite transplantation of a split-thickness skin graft (STSG) combined ... with an acellular dermal matrix (ADM) is a promising repair method for full-thickness skin defects. Due to delayed vascularization ...

Abstract	Background: The composite transplantation of a split-thickness skin graft (STSG) combined with an acellular dermal matrix (ADM) is a promising repair method for full-thickness skin defects. Due to delayed vascularization of the ADM, no currently available engineered skin tissue is able to permanently cover full-thickness skin defects via a single-stage procedure. Epidermal stem cells (EpSCs) have been found to promote angiogenesis in the wound bed. Whether EpSCs can induce early angiogenesis of dermal substitutes and promote the survival of single-stage tissue-engineered skin transplantation needs to be further studied. Methods: In vitro, rat vascular endothelial cells (RVECs) were treated with the supernatant of EpSCs cultured in ADM and stimulated for 48 h. RVECs were analysed by RNA sequencing and tube formation assays. For the in vivo experiment, 75 rats were randomly divided into five groups: ADM, ADM + EpSCs (AE), STSG, ADM + STSG (AS), and ADM + STSG + EpSCs (ASE) groups. The quality of wound healing was estimated by general observation and H&E and Masson staining. The blood perfusion volume was evaluated using the LDPI system, and the expression of vascular markers was determined by immunohistochemistry (IHC). Results: The active substances secreted by EpSCs cultured in ADM promoted angiogenesis, as shown by tube formation experiments and RNA-seq. EpSCs promoted epithelialization of the ADM and vascularization of the ADM implant. The ASE group showed significantly increased skin graft survival, reduced skin contraction, and an improved cosmetic appearance compared with the AS group and the STSG control group. Conclusions: In summary, our findings suggest that EpSCs promote the formation of new blood vessels in dermal substitutes and support one-step transplantation of tissue-engineered skin, and thereby provide new ideas for clinical application.
MeSH term(s)	Rats ; Animals ; Wound Healing ; Endothelial Cells ; Skin, Artificial ; Skin ; Stem Cells
Language	English
Publishing date	2023-03-23
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2548671-8
ISSN	1757-6512 ; 1757-6512
ISSN (online)	1757-6512
ISSN	1757-6512
DOI	10.1186/s13287-023-03281-z
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Article ; Online: Cleavage Under Targets & Release Using Nuclease (CUT&RUN) of Histone Modifications in Epidermal Stem Cells of Adult Murine Skin.

Flora, Pooja / Ezhkova, Elena

Methods in molecular biology (Clifton, N.J.)

2023 Volume 2736, Page(s) 9–21

Abstract: ... in fluorescence-activated cell sorting (FACS)-isolated epidermal stem cells from adult mice. ... in many cell types as it utilizes far less cell numbers when compared to Chromatin Immunoprecipitation ...

Abstract	Cleavage Under Targets & Release Using Nuclease (CUT&RUN) has swiftly become the preferred procedure over the past few years for genomic mapping and detecting interactions between chromatin and its bound proteins. CUT&RUN is now being widely used for characterizing the epigenetic landscape in many cell types as it utilizes far less cell numbers when compared to Chromatin Immunoprecipitation-sequencing (ChIP-seq), thereby making it a powerful tool for researchers working with limited material. This protocol has been specifically optimized for detecting histone modifications in fluorescence-activated cell sorting (FACS)-isolated epidermal stem cells from adult mice.
MeSH term(s)	Mice ; Animals ; Histone Code ; Chromatin ; Protein Processing, Post-Translational ; Stem Cells ; Epigenomics/methods
Chemical Substances	Chromatin
Language	English
Publishing date	2023-08-24
Publishing country	United States
Document type	Journal Article ; Research Support, N.I.H., Extramural
ISSN	1940-6029
ISSN (online)	1940-6029
DOI	10.1007/7651_2023_499
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Article ; Online: Morroniside promotes skin wound re-epithelialization by facilitating epidermal stem cell proliferation through GLP-1R-mediated upregulation of β-catenin expression.

Yu, Chenghao / Yu, Siyuan / Liu, Zuohua / Xu, Lei / Zhang, Zhiqiang / Wan, Jiaming / Ji, Pengxiang / Zhang, Ping / Fu, Yi / Le, Yingying / Hou, Ruixing

Acta biochimica et biophysica Sinica

2024

Abstract: Epidermal stem cells (EpSCs) play a vital role in skin wound healing through re-epithelialization ... Identifying chemicals that can promote EpSC proliferation is helpful for treating skin wounds. This study ... mechanisms. Application of 10‒50 μg/mL of morroniside to the skin wound promotes wound healing in mice. ...

Abstract	Epidermal stem cells (EpSCs) play a vital role in skin wound healing through re-epithelialization. Identifying chemicals that can promote EpSC proliferation is helpful for treating skin wounds. This study investigates the effect of morroniside on cutaneous wound healing in mice and explores the underlying mechanisms. Application of 10‒50 μg/mL of morroniside to the skin wound promotes wound healing in mice.
Language	English
Publishing date	2024-05-22
Publishing country	China
Document type	Journal Article
ZDB-ID	2175256-4
ISSN	1745-7270 ; 0582-9879 ; 1672-9145
ISSN (online)	1745-7270
ISSN	0582-9879 ; 1672-9145
DOI	10.3724/abbs.2024070
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Article ; Online: Differential gene screening and bioinformatics analysis of epidermal stem cells and dermal fibroblasts during skin aging.

Hu, Weisheng / Jing, Yuan / Yu, Qingqian / Huang, Ning

Scientific reports

2022 Volume 12, Issue 1, Page(s) 12019

Abstract: ... GSE110978 and GSE117763 were downloaded from GEO database, and epidermal stem cells and skin aging related ... of skin aging in GEO database by bioinformatics methods. Dermal fibroblasts and skin aging related data sets ... for candidate drugs. Try to explore the mechanism of hub gene action in skin aging progression, and to discover ...

Abstract	To explore the differentially expressed genes (DEGs) and potential therapeutic targets of skin aging in GEO database by bioinformatics methods. Dermal fibroblasts and skin aging related data sets GSE110978 and GSE117763 were downloaded from GEO database, and epidermal stem cells and skin aging related data sets GSE137176 were downloaded. GEO2R was used to screen DEGs of candidate samples from the three microarrays, GO function analysis and KEGG pathway analysis were performed. Protein interaction network was constructed using String database, and hub gene was obtained by Cytoscape. NetworkAnalys was used to analyze the coregulatory network of DEGs and MicroRNA (miRNA), interaction with TF, and protein-chemical interactions of DEGs. Finally, DSigDB was used to determine candidate drugs for DEGs. Six DEGs were obtained. It mainly involves the cytological processes such as response to metal ion, and is enriched in mineral absorption and other signal pathways. Ten genes were screened by PPI analysis. Gene-miRNA coregulatory network found that Peg3 and mmu-miR-1931 in DEGs were related to each other, and Cybrd1 was related to mmu-miR-290a-5p and mmu-miR-3082-5p. TF-gene interactions found that the transcription factor UBTF co-regulated two genes, Arhgap24 and Mpzl1. Protein-chemical Interactions analysis and identification of candidate drugs show results for candidate drugs. Try to explore the mechanism of hub gene action in skin aging progression, and to discover the key signaling pathways leading to skin aging, which may be a high risk of skin aging.
MeSH term(s)	Computational Biology/methods ; Fibroblasts/metabolism ; Gene Expression Profiling/methods ; Gene Expression Regulation, Neoplastic ; Gene Regulatory Networks ; MicroRNAs/genetics ; MicroRNAs/metabolism ; Protein Interaction Maps/genetics ; Skin Aging/genetics ; Stem Cells/metabolism
Chemical Substances	MicroRNAs
Language	English
Publishing date	2022-07-14
Publishing country	England
Document type	Journal Article ; Research Support, Non-U.S. Gov't
ZDB-ID	2615211-3
ISSN	2045-2322 ; 2045-2322
ISSN (online)	2045-2322
ISSN	2045-2322
DOI	10.1038/s41598-022-16314-z
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