Article: Influence of neopterin and 7,8-dihydroneopterin on the replication of Coxsackie type B5 and influenza A viruses.
Medical microbiology and immunology
2007 Volume 196, Issue 1, Page(s) 23–29
Abstract: ... to investigate the effect of neopterin and 7,8-dihydroneopterin on the replication of Coxsackie type B5 and ... significantly suppressed the propagation of influenza A virus. Neopterin and 7,8-dihydroneopterin were ... of neopterin and 7,8-dihydroneopterin on virus replication apparently are different. ...
| Abstract | Pteridine derivatives neopterin and 7,8-dihydroneopterin are produced by human macrophages and dendritic cells upon stimulation with interferon-gamma (IFN-gamma) and therefore become detectable in increased amounts in humans during cell-mediated (Th1-type) immune response. Compounds produced upon influence of cytokine IFN-gamma often exert antiproliferative and antiviral activity. The aim of this study was to investigate the effect of neopterin and 7,8-dihydroneopterin on the replication of Coxsackie type B5 and influenza A viruses. The changes in the replication of these viruses were evaluated by the degree of cytopathic effect and their ability to form plaques in Coxsackie B5-infected human larynx carcinoma epithelial (Hep-2) cells and in influenza A-infected canine kidney epithelial cells (MDCK). Potential toxicity of neopterin and 7,8-dihydroneopterin was estimated by the incorporation of (3)H-thymidine and (3)H-uridine into Hep-2 and MDCK cells. Whereas 30 nmol/l neopterin delayed the development of the cytopathic effect of Coxsackie B5 virus in Hep-2 cells (P < 0.01), 7,8-dihydroneopterin did not have any essential influence at any of the concentrations tested between 10 nmol/l and 1,000 micromol/l. However, 100-1,500 micromol/l 7,8-dihydroneopterin significantly suppressed the propagation of influenza A virus. Neopterin and 7,8-dihydroneopterin were practically nontoxic for Hep-2 and MDCK cells even at high microM concentration. Results suggest that the increased production of neopterin derivatives by activated macrophages and dendritic cells may represent part of the antiviral armature induced by IFN-gamma. The mechanisms of the inhibitory effects of neopterin and 7,8-dihydroneopterin on virus replication apparently are different. |
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| MeSH term(s) | Animals ; Cell Line ; Cell Line, Tumor ; Cytopathogenic Effect, Viral/drug effects ; Dogs ; Enterovirus B, Human/drug effects ; Enterovirus B, Human/physiology ; Humans ; Influenza A Virus, H3N2 Subtype/drug effects ; Influenza A Virus, H3N2 Subtype/physiology ; Neopterin/analogs & derivatives ; Neopterin/metabolism ; Neopterin/pharmacology ; Neopterin/toxicity ; Viral Plaque Assay ; Virus Replication/drug effects | |||||
| Chemical Substances | 7,8-dihydroneopterin (1218-98-0) ; Neopterin (670-65-5) | |||||
| Language | English | |||||
| Publishing date | 2007-03 | |||||
| Publishing country | Germany | |||||
| Document type | Journal Article ; Research Support, Non-U.S. Gov't | |||||
| ZDB-ID | 120933-4 | |||||
| ISSN | 1432-1831 ; 0300-8584 | |||||
| ISSN (online) | 1432-1831 | |||||
| ISSN | 0300-8584 | |||||
| DOI | 10.1007/s00430-006-0025-y | |||||
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| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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