Article ; Online: Genetic variants in TRPM7 associated with unexplained stillbirth modify ion channel function.
2019 Volume 29, Issue 11, Page(s) 1797–1807
Abstract: ... found in the unexplained stillbirth population adversely affect ion channel function and ... on TRPM7 ion channel function in heterologous cells. We also used cardiomyocytes derived from human ... variants, p.G179V and p.T860M, lead to a marked reduction in ion channel conductance. This observation was ...
| Abstract | Stillbirth is the loss of a fetus after 22 weeks of gestation, of which almost half go completely unexplained despite post-mortem. We recently sequenced 35 arrhythmia-associated genes from 70 unexplained stillbirth cases. Our hypothesis was that deleterious mutations in channelopathy genes may have a functional effect in utero that may be pro-arrhythmic in the developing fetus. We observed four heterozygous, nonsynonymous variants in transient receptor potential melastatin 7 (TRPM7), a ubiquitously expressed ion channel known to regulate cardiac development and repolarization in mice. We used site-directed mutagenesis and single-cell patch-clamp to analyze the functional effect of the four stillbirth mutants on TRPM7 ion channel function in heterologous cells. We also used cardiomyocytes derived from human pluripotent stem cells to model the contribution of TRPM7 to action potential morphology. Our results show that two TRPM7 variants, p.G179V and p.T860M, lead to a marked reduction in ion channel conductance. This observation was underpinned by a lack of measurable TRPM7 protein expression, which in the case of p.T860M was due to rapid proteasomal degradation. We also report that human hiPSC-derived cardiomyocytes possess measurable TRPM7 currents; however, siRNA knockdown did not directly affect action potential morphology. TRPM7 variants found in the unexplained stillbirth population adversely affect ion channel function and this may precipitate fatal arrhythmia in utero. |
|||||
|---|---|---|---|---|---|---|
| MeSH term(s) | Aborted Fetus/physiopathology ; Animals ; Arrhythmias, Cardiac/genetics ; Arrhythmias, Cardiac/pathology ; Cell Differentiation/genetics ; Gene Expression Regulation, Developmental/genetics ; Genetic Predisposition to Disease ; Heart/growth & development ; Heart/physiopathology ; Humans ; Induced Pluripotent Stem Cells/metabolism ; Induced Pluripotent Stem Cells/pathology ; Ion Channels/genetics ; Mice ; Mutation/genetics ; Myocytes, Cardiac/metabolism ; Myocytes, Cardiac/pathology ; Protein-Serine-Threonine Kinases/genetics ; Stillbirth/genetics ; TRPM Cation Channels/genetics | |||||
| Chemical Substances | Ion Channels ; TRPM Cation Channels ; Protein-Serine-Threonine Kinases (EC 2.7.11.1) ; TRPM7 protein, human (EC 2.7.11.1) | |||||
| Language | English | |||||
| Publishing date | 2019-08-13 | |||||
| Publishing country | England | |||||
| Document type | Journal Article ; Research Support, Non-U.S. Gov't | |||||
| ZDB-ID | 1108742-0 | |||||
| ISSN | 1460-2083 ; 0964-6906 | |||||
| ISSN (online) | 1460-2083 | |||||
| ISSN | 0964-6906 | |||||
| DOI | 10.1093/hmg/ddz198 | |||||
| Shelf mark |
|
|||||
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
More links
Kategorien
In stock of ZB MED Cologne/Königswinter
| Zs.A 3469: Show issues | Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (2.OG) ab Jg. 2022: Lesesaal (EG) |
Order via subito
This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.