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  1. Article ; Online: Expression of concern: Microchip-based structure determination of low-molecular weight proteins using cryo-electron microscopy.

    Casasanta, Michael A / Jonaid, G M / Kaylor, Liam / Luqiu, William Y / Solares, Maria J / Schroen, Mariah L / Dearnaley, William J / Wilson, Jarad / Dukes, Madeline J / Kelly, Deborah F

    Nanoscale

    2024  Volume 16, Issue 9, Page(s) 4919

    Abstract: Expression of concern for 'Microchip-based structure determination of low-molecular weight proteins ... using cryo-electron microscopy' by Michael A. Casasanta ...

    Abstract Expression of concern for 'Microchip-based structure determination of low-molecular weight proteins using cryo-electron microscopy' by Michael A. Casasanta
    Language English
    Publishing date 2024-02-29
    Publishing country England
    Document type Expression of Concern
    ZDB-ID 2515664-0
    ISSN 2040-3372 ; 2040-3364
    ISSN (online) 2040-3372
    ISSN 2040-3364
    DOI 10.1039/d4nr90037e
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Microchip-based structure determination of low-molecular weight proteins using cryo-electron microscopy.

    Casasanta, Michael A / Jonaid, G M / Kaylor, Liam / Luqiu, William Y / Solares, Maria J / Schroen, Mariah L / Dearnaley, William J / Wilson, Jarad / Dukes, Madeline J / Kelly, Deborah F

    Nanoscale

    2021  Volume 13, Issue 15, Page(s) 7285–7293

    Abstract: ... a microchip-based toolkit to perform complementary structural and biochemical analysis on low-molecular weight ... and important role in therapeutic development. Cryo-EM structures of the N protein monomer revealed ... Interest in cryo-Electron Microscopy (EM) imaging has skyrocketed in recent years due ...

    Abstract Interest in cryo-Electron Microscopy (EM) imaging has skyrocketed in recent years due to its pristine views of macromolecules and materials. As advances in instrumentation and computing algorithms spurred this progress, there is renewed focus to address specimen-related challenges. Here we contribute a microchip-based toolkit to perform complementary structural and biochemical analysis on low-molecular weight proteins. As a model system, we used the SARS-CoV-2 nucleocapsid (N) protein (48 kDa) due to its stability and important role in therapeutic development. Cryo-EM structures of the N protein monomer revealed a flexible N-terminal "top hat" motif and a helical-rich C-terminal domain. To complement our structural findings, we engineered microchip-based immunoprecipitation assays that led to the discovery of the first antibody binding site on the N protein. The data also facilitated molecular modeling of a variety of pandemic and common cold-related coronavirus proteins. Such insights may guide future pandemic-preparedness protocols through immuno-engineering strategies to mitigate viral outbreaks.
    MeSH term(s) Coronavirus Nucleocapsid Proteins/chemistry ; Cryoelectron Microscopy ; Molecular Weight ; Phosphoproteins/chemistry ; Protein Structure, Secondary ; SARS-CoV-2/chemistry
    Chemical Substances Coronavirus Nucleocapsid Proteins ; Phosphoproteins ; nucleocapsid phosphoprotein, SARS-CoV-2
    Language English
    Publishing date 2021-04-12
    Publishing country England
    Document type Journal Article
    ZDB-ID 2515664-0
    ISSN 2040-3372 ; 2040-3364
    ISSN (online) 2040-3372
    ISSN 2040-3364
    DOI 10.1039/d1nr00388g
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

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