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  1. Article ; Online: TRPM7 and MagT1 in the osteogenic differentiation of human mesenchymal stem cells in vitro.

    Castiglioni, Sara / Romeo, Valentina / Locatelli, Laura / Cazzaniga, Alessandra / Maier, Jeanette A M

    Scientific reports

    2018  Volume 8, Issue 1, Page(s) 16195

    Abstract: ... mesenchymal stem cells. TRPM7 and MagT1 are upregulated in osteogenic differentiation and silencing either one ... and the magnesium transporter MagT1 have a role in harmonizing the osteogenic differentiation of human ... to microenvironmental stimuli by undergoing osteogenic differentiation. We show that the kinase and cation channel TRPM7 ...

    Abstract Mesenchymal stem cells are fundamental for bone formation and repair since they respond to microenvironmental stimuli by undergoing osteogenic differentiation. We show that the kinase and cation channel TRPM7 and the magnesium transporter MagT1 have a role in harmonizing the osteogenic differentiation of human mesenchymal stem cells. TRPM7 and MagT1 are upregulated in osteogenic differentiation and silencing either one accelerates osteogenic differentiation, partly through the activation of autophagy. Intriguingly, similar results were obtained when the cells were cultured under magnesium deficient conditions. These results underpin the contribution of magnesium, TRPM7 and MagT1 to autophagy and osteoblastogenesis.
    MeSH term(s) Autophagy/genetics ; Cation Transport Proteins/genetics ; Cell Differentiation/genetics ; Cells, Cultured ; Gene Expression Regulation, Developmental/genetics ; Gene Silencing ; Humans ; Magnesium/metabolism ; Mesenchymal Stem Cells/metabolism ; Osteogenesis/genetics ; Protein-Serine-Threonine Kinases/genetics ; TRPM Cation Channels/genetics
    Chemical Substances Cation Transport Proteins ; MagT1 protein, human ; TRPM Cation Channels ; Protein-Serine-Threonine Kinases (EC 2.7.11.1) ; TRPM7 protein, human (EC 2.7.11.1) ; Magnesium (I38ZP9992A)
    Language English
    Publishing date 2018-11-01
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2615211-3
    ISSN 2045-2322 ; 2045-2322
    ISSN (online) 2045-2322
    ISSN 2045-2322
    DOI 10.1038/s41598-018-34324-8
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: TRPM7 and MagT1 in the osteogenic differentiation of human mesenchymal stem cells in vitro

    Sara Castiglioni / Valentina Romeo / Laura Locatelli / Alessandra Cazzaniga / Jeanette A. M. Maier

    Scientific Reports, Vol 8, Iss 1, Pp 1-

    2018  Volume 10

    Abstract: ... mesenchymal stem cells. TRPM7 and MagT1 are upregulated in osteogenic differentiation and silencing either one ... and the magnesium transporter MagT1 have a role in harmonizing the osteogenic differentiation of human ... to microenvironmental stimuli by undergoing osteogenic differentiation. We show that the kinase and cation channel TRPM7 ...

    Abstract Abstract Mesenchymal stem cells are fundamental for bone formation and repair since they respond to microenvironmental stimuli by undergoing osteogenic differentiation. We show that the kinase and cation channel TRPM7 and the magnesium transporter MagT1 have a role in harmonizing the osteogenic differentiation of human mesenchymal stem cells. TRPM7 and MagT1 are upregulated in osteogenic differentiation and silencing either one accelerates osteogenic differentiation, partly through the activation of autophagy. Intriguingly, similar results were obtained when the cells were cultured under magnesium deficient conditions. These results underpin the contribution of magnesium, TRPM7 and MagT1 to autophagy and osteoblastogenesis.
    Keywords Medicine ; R ; Science ; Q
    Language English
    Publishing date 2018-11-01T00:00:00Z
    Publisher Nature Publishing Group
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article ; Online: The simultaneous downregulation of TRPM7 and MagT1 in human mesenchymal stem cells in vitro: Effects on growth and osteogenic differentiation.

    Castiglioni, Sara / Romeo, Valentina / Locatelli, Laura / Zocchi, Monica / Zecchini, Silvia / Maier, Jeanette A M

    Biochemical and biophysical research communications

    2019  Volume 513, Issue 1, Page(s) 159–165

    Abstract: ... mesenchymal stem cells. Here we demonstrate that the simultaneous downregulation of TRPM7 and MagT1 inhibits cell ... that silencing either TRPM7 or MagT1 accelerates the osteogenic differentiation of human bone ... growth and activates autophagy, which is required in the early phases of osteoblastogenesis. In TRPM7/MagT1 ...

    Abstract The magnesium transporters TRPM7 and MagT1 are overexpressed in osteoblastogenesis. We have shown that silencing either TRPM7 or MagT1 accelerates the osteogenic differentiation of human bone mesenchymal stem cells. Here we demonstrate that the simultaneous downregulation of TRPM7 and MagT1 inhibits cell growth and activates autophagy, which is required in the early phases of osteoblastogenesis. In TRPM7/MagT1 downregulating cells the expression of two transcription factors required for activating osteogenesis, i.e. RUNX2 and OSTERIX, is induced more than in the controls both in the presence and in the absence of osteogenic stimuli, while COL1A1 is upregulated in co-silencing cells as much as in the controls. This explains why we found no differences in calcium deposition. We conclude that one of the two transporters should be expressed to accelerate osteogenic differentiation.
    MeSH term(s) Adult ; Autophagy ; Cation Transport Proteins/genetics ; Cation Transport Proteins/metabolism ; Cell Differentiation ; Cell Proliferation ; Cells, Cultured ; Down-Regulation ; Humans ; Mesenchymal Stem Cells/cytology ; Mesenchymal Stem Cells/metabolism ; Osteogenesis ; Protein-Serine-Threonine Kinases/genetics ; Protein-Serine-Threonine Kinases/metabolism ; RNA Interference ; TRPM Cation Channels/genetics ; TRPM Cation Channels/metabolism
    Chemical Substances Cation Transport Proteins ; MagT1 protein, human ; TRPM Cation Channels ; Protein-Serine-Threonine Kinases (EC 2.7.11.1) ; TRPM7 protein, human (EC 2.7.11.1)
    Language English
    Publishing date 2019-04-02
    Publishing country United States
    Document type Journal Article
    ZDB-ID 205723-2
    ISSN 1090-2104 ; 0006-291X ; 0006-291X
    ISSN (online) 1090-2104 ; 0006-291X
    ISSN 0006-291X
    DOI 10.1016/j.bbrc.2019.03.178
    Database MEDical Literature Analysis and Retrieval System OnLINE

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 116: Show issues Location:
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    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
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  4. Article: The simultaneous downregulation of TRPM7 and MagT1 in human mesenchymal stem cells in vitro: Effects on growth and osteogenic differentiation

    Castiglioni, Sara / Romeo, Valentina / Locatelli, Laura / Zocchi, Monica / Zecchini, Silvia / Maier, Jeanette A.M

    Biochemical and biophysical research communications. 2019 May 21, v. 513, no. 1

    2019  

    Abstract: ... mesenchymal stem cells. Here we demonstrate that the simultaneous downregulation of TRPM7 and MagT1 inhibits cell ... that silencing either TRPM7 or MagT1 accelerates the osteogenic differentiation of human bone ... growth and activates autophagy, which is required in the early phases of osteoblastogenesis. In TRPM7/MagT1 ...

    Abstract The magnesium transporters TRPM7 and MagT1 are overexpressed in osteoblastogenesis. We have shown that silencing either TRPM7 or MagT1 accelerates the osteogenic differentiation of human bone mesenchymal stem cells. Here we demonstrate that the simultaneous downregulation of TRPM7 and MagT1 inhibits cell growth and activates autophagy, which is required in the early phases of osteoblastogenesis. In TRPM7/MagT1 downregulating cells the expression of two transcription factors required for activating osteogenesis, i.e. RUNX2 and OSTERIX, is induced more than in the controls both in the presence and in the absence of osteogenic stimuli, while COL1A1 is upregulated in co-silencing cells as much as in the controls. This explains why we found no differences in calcium deposition. We conclude that one of the two transporters should be expressed to accelerate osteogenic differentiation.
    Keywords autophagy ; bone formation ; calcium ; cell growth ; gene expression regulation ; humans ; magnesium ; mesenchymal stromal cells ; transcription factors ; transporters
    Language English
    Dates of publication 2019-0521
    Size p. 159-165.
    Publishing place Elsevier Inc.
    Document type Article
    ZDB-ID 205723-2
    ISSN 0006-291X ; 0006-291X
    ISSN (online) 0006-291X
    ISSN 0006-291X
    DOI 10.1016/j.bbrc.2019.03.178
    Database NAL-Catalogue (AGRICOLA)

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    In stock of ZB MED Bonn / Germany

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