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  1. Article ; Online: Specific Integration of Temperate Phage Decreases the Pathogenicity of Host Bacteria.

    Chen, Yibao / Yang, Lan / Yang, Dan / Song, Jiaoyang / Wang, Can / Sun, Erchao / Gu, Changqin / Chen, Huanchun / Tong, Yigang / Tao, Pan / Wu, Bin

    Frontiers in cellular and infection microbiology

    2020  Volume 10, Page(s) 14

    Abstract: ... of host bacteria. Many virulence genes of pathogenic bacteria were identified in temperate phage genomes ... supporting the concept that temperate phages play important roles in increasing the bacterial pathogenicity ... Temperate phages are considered as natural vectors for gene transmission among bacteria due ...

    Abstract Temperate phages are considered as natural vectors for gene transmission among bacteria due to the ability to integrate their genomes into a host chromosome, therefore, affect the fitness and phenotype of host bacteria. Many virulence genes of pathogenic bacteria were identified in temperate phage genomes, supporting the concept that temperate phages play important roles in increasing the bacterial pathogenicity through delivery of the virulence genes. However, little is known about the roles of temperate phages in attenuation of bacterial virulence. Here, we report a novel
    MeSH term(s) Animals ; Bacterial Vaccines/immunology ; Bordetella Infections/microbiology ; Bordetella Infections/prevention & control ; Bordetella bronchiseptica/growth & development ; Bordetella bronchiseptica/immunology ; Bordetella bronchiseptica/pathogenicity ; Bordetella bronchiseptica/virology ; DNA, Viral/genetics ; Female ; Genome, Viral ; Lysogeny ; Mice ; Mice, Inbred BALB C ; Phylogeny ; Prophages/genetics ; Prophages/physiology ; Siphoviridae/classification ; Siphoviridae/genetics ; Siphoviridae/isolation & purification ; Siphoviridae/physiology ; Vaccines, Attenuated/immunology ; Virulence
    Chemical Substances Bacterial Vaccines ; DNA, Viral ; Vaccines, Attenuated
    Keywords covid19
    Language English
    Publishing date 2020-02-04
    Publishing country Switzerland
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 2619676-1
    ISSN 2235-2988 ; 2235-2988
    ISSN (online) 2235-2988
    ISSN 2235-2988
    DOI 10.3389/fcimb.2020.00014
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: Specific Integration of Temperate Phage Decreases the Pathogenicity of Host Bacteria

    Chen, Yibao / Yang, Lan / Yang, Dan / Song, Jiaoyang / Wang, Can / Sun, Erchao / Gu, Changqin / Chen, Huanchun / Tong, Yigang / Tao, Pan / Wu, Bin

    Frontiers in Cellular and Infection Microbiology, 10:14

    2020  

    Abstract: ... of host bacteria. Many virulence genes of pathogenic bacteria were identified in temperate phage genomes ... supporting the concept that temperate phages play important roles in increasing the bacterial pathogenicity ... in attenuation of bacterial virulence. Here, we report a novel Bordetella bronchiseptica temperate phage, vB_BbrS ...

    Abstract Temperate phages are considered as natural vectors for gene transmission among bacteria due to the ability to integrate their genomes into a host chromosome, therefore, affect the fitness and phenotype of host bacteria. Many virulence genes of pathogenic bacteria were identified in temperate phage genomes, supporting the concept that temperate phages play important roles in increasing the bacterial pathogenicity through delivery of the virulence genes. However, little is known about the roles of temperate phages in attenuation of bacterial virulence. Here, we report a novel Bordetella bronchiseptica temperate phage, vB_BbrS_PHB09 (PHB09), which has a 42,129-bp dsDNA genome with a G+C content of 62.8%. Phylogenetic analysis based on large terminase subunit indicated that phage PHB09 represented a new member of the family Siphoviridae. The genome of PHB09 contains genes encoding lysogen-associated proteins, including integrase and cI protein. The integration site of PHB09 is specifically located within a pilin gene of B. bronchiseptica. Importantly, we found that the integration of phage PHB09 significantly decreased the virulence of parental strain B. bronchiseptica Bb01 in mice, most likely through disruption the expression of pilin gene. Moreover, a single shot of the prophage bearing B. bronchiseptica strain completely protected mice against lethal challenge with wild-type virulent B. bronchiseptica, indicating the vaccine potential of lysogenized strain. Our findings not only indicate the complicated roles of temperate phages in bacterial virulence other than simple delivery of virulent genes but also provide a potential strategy for developing bacterial vaccines.
    Keywords COVID-19 ; temperate phage ; attenuation ; bacterial virulence ; bordetella bronchiseptica ; integration site ; covid19
    Language English
    Publishing country de
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article: Specific Integration of Temperate Phage Decreases the Pathogenicity of Host Bacteria

    Frontiers in Cellular and Infection Microbiology, 10:14

    2020  

    Abstract: ... of host bacteria. Many virulence genes of pathogenic bacteria were identified in temperate phage genomes ... supporting the concept that temperate phages play important roles in increasing the bacterial pathogenicity ... in attenuation of bacterial virulence. Here, we report a novel Bordetella bronchiseptica temperate phage, vB_BbrS ...

    Abstract Temperate phages are considered as natural vectors for gene transmission among bacteria due to the ability to integrate their genomes into a host chromosome, therefore, affect the fitness and phenotype of host bacteria. Many virulence genes of pathogenic bacteria were identified in temperate phage genomes, supporting the concept that temperate phages play important roles in increasing the bacterial pathogenicity through delivery of the virulence genes. However, little is known about the roles of temperate phages in attenuation of bacterial virulence. Here, we report a novel Bordetella bronchiseptica temperate phage, vB_BbrS_PHB09 (PHB09), which has a 42,129-bp dsDNA genome with a G+C content of 62.8%. Phylogenetic analysis based on large terminase subunit indicated that phage PHB09 represented a new member of the family Siphoviridae. The genome of PHB09 contains genes encoding lysogen-associated proteins, including integrase and cI protein. The integration site of PHB09 is specifically located within a pilin gene of B. bronchiseptica. Importantly, we found that the integration of phage PHB09 significantly decreased the virulence of parental strain B. bronchiseptica Bb01 in mice, most likely through disruption the expression of pilin gene. Moreover, a single shot of the prophage bearing B. bronchiseptica strain completely protected mice against lethal challenge with wild-type virulent B. bronchiseptica, indicating the vaccine potential of lysogenized strain. Our findings not only indicate the complicated roles of temperate phages in bacterial virulence other than simple delivery of virulent genes but also provide a potential strategy for developing bacterial vaccines.
    Keywords attenuation ; bordetella bronchiseptica ; bacterial virulence ; integration site ; temperate phage
    Language English
    Document type Article
    Database Repository for Life Sciences

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    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

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