Artikel ; Online: Rapid and Sensitive Detection of anti-SARS-CoV-2 IgG, Using Lanthanide-Doped Nanoparticles-Based Lateral Flow Immunoassay.
2020 Band 92, Heft 10, Seite(n) 7226–7231
Abstract: ... with those obtained by RT-PCR. Thus, this assay can achieve rapid and sensitive detection of anti-SARS-CoV-2 IgG ... LFIA) that uses lanthanide-doped polysterene nanoparticles (LNPs) to detect anti-SARV-CoV-2 IgG ... to identify positive cases. Here we report the development of a rapid and sensitive lateral flow immunoassay ...
| Abstract | The outbreak of 2019 coronavirus disease (COVID-19) has been a challenge for hospital laboratories because of the huge number of samples that must be tested for the presence of the causative pathogen, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Simple and rapid immunodiagnostic methods are urgently needed to identify positive cases. Here we report the development of a rapid and sensitive lateral flow immunoassay (LFIA) that uses lanthanide-doped polysterene nanoparticles (LNPs) to detect anti-SARV-CoV-2 IgG in human serum. A recombinant nucleocapsid phosphoprotein of SARS-CoV-2 was dispensed onto a nitrocellulose membrane to capture specific IgG. Mouse anti-human IgG antibody was labeled with self-assembled LNPs that served as a fluorescent reporter. A 100-μL aliquot of serum samples (1:1000 dilution) was used for this assay and the whole detection process took 10 min. The results of the validation experiment met the requirements for clinical diagnostic reagents. A value of 0.0666 was defined as the cutoff value by assaying 51 normal samples. We tested 7 samples that were positive by reverse-transcription (RT-)PCR and 12 that were negative but clinically suspicious for the presence of anti-SARS-CoV-2 IgG. One of the negative samples was determined to be SARS-CoV-2 IgG positive, while the results for the other samples were consistent with those obtained by RT-PCR. Thus, this assay can achieve rapid and sensitive detection of anti-SARS-CoV-2 IgG in human serum and allow positive identification in suspicious cases; it can also be useful for monitoring the progression COVID-19 and evaluating patients' response to treatment. |
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| Mesh-Begriff(e) | Antibodies, Viral/blood ; Betacoronavirus/immunology ; COVID-19 ; Coronavirus Infections/blood ; Coronavirus Infections/diagnosis ; Humans ; Immunoassay/methods ; Immunoglobulin G/blood ; Lanthanoid Series Elements/chemistry ; Metal Nanoparticles/chemistry ; Nanoparticles ; Pandemics ; Pneumonia, Viral/blood ; Pneumonia, Viral/diagnosis ; SARS-CoV-2 ; Sensitivity and Specificity |
| Chemische Substanzen | Antibodies, Viral ; Immunoglobulin G ; Lanthanoid Series Elements |
| Schlagwörter | covid19 |
| Sprache | Englisch |
| Erscheinungsdatum | 2020-05-05 |
| Erscheinungsland | United States |
| Dokumenttyp | Journal Article ; Research Support, Non-U.S. Gov't ; Validation Study |
| ZDB-ID | 1508-8 |
| ISSN | 1520-6882 ; 0003-2700 |
| ISSN (online) | 1520-6882 |
| ISSN | 0003-2700 |
| DOI | 10.1021/acs.analchem.0c00784 |
| Datenquelle | MEDical Literature Analysis and Retrieval System OnLINE |
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