Article ; Online: Antigen production and development of an indirect ELISA based on the nucleocapsid protein to detect human SARS-CoV-2 seroconversion.
Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology
2021 Volume 52, Issue 4, Page(s) 2069–2073
Abstract: ... immunization to future vaccines. Here, the SARS-CoV-2 nucleocapsid protein was expressed in Escherichia coli ... to track COVID-19 cases and determine the level of humoral response to SARS-CoV-2 infections and/or ... and purified to homogeneity and high yield using a single chromatography step. The purified SARS-CoV-2 ...
| Abstract | Serological assays are important tools to identify previous exposure to SARS-CoV-2, helping to track COVID-19 cases and determine the level of humoral response to SARS-CoV-2 infections and/or immunization to future vaccines. Here, the SARS-CoV-2 nucleocapsid protein was expressed in Escherichia coli and purified to homogeneity and high yield using a single chromatography step. The purified SARS-CoV-2 nucleocapsid protein was used to develop an indirect enzyme-linked immunosorbent assay for the identification of human SARS-CoV-2 seroconverts. The assay sensitivity and specificity were determined analyzing sera from 140 RT-qPCR-confirmed COVID-19 cases and 210 pre-pandemic controls. The assay operated with 90% sensitivity and 98% specificity; identical accuracies were obtained in head-to-head comparison with a commercial ELISA kit. Antigen-coated plates were stable for up to 3 months at 4 °C. The ELISA method described is ready for mass production and will be an additional tool to track COVID-19 cases. |
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| MeSH term(s) | Antibodies, Viral/blood ; COVID-19/diagnosis ; COVID-19/immunology ; Coronavirus Nucleocapsid Proteins/immunology ; Enzyme-Linked Immunosorbent Assay ; Humans ; Immunity, Humoral ; Nucleocapsid Proteins/genetics ; Phosphoproteins/immunology ; Sensitivity and Specificity ; Seroconversion |
| Chemical Substances | Antibodies, Viral ; Coronavirus Nucleocapsid Proteins ; Nucleocapsid Proteins ; Phosphoproteins ; nucleocapsid phosphoprotein, SARS-CoV-2 |
| Language | English |
| Publishing date | 2021-08-03 |
| Publishing country | Brazil |
| Document type | Journal Article |
| ZDB-ID | 2017175-4 |
| ISSN | 1678-4405 ; 1517-8382 |
| ISSN (online) | 1678-4405 |
| ISSN | 1517-8382 |
| DOI | 10.1007/s42770-021-00556-6 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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