Article: Glutathione Fine-Tunes the Innate Immune Response toward Antiviral Pathways in a Macrophage Cell Line Independently of Its Antioxidant Properties.
2017 Volume 8, Page(s) 1239
Abstract: ... to limit inflammation but to fine-tune the innate immune response to infection. ... of GSH does not exacerbate the inflammatory gene expression profile in the RAW264 macrophage cell line or ... Glutathione (GSH), a major cellular antioxidant, is considered an inhibitor of the inflammatory ...
| Abstract | Glutathione (GSH), a major cellular antioxidant, is considered an inhibitor of the inflammatory response involving reactive oxygen species (ROS). However, evidence is largely based on experiments with exogenously added antioxidants/reducing agents or pro-oxidants. We show that depleting macrophages of 99% of GSH does not exacerbate the inflammatory gene expression profile in the RAW264 macrophage cell line or increase expression of inflammatory cytokines in response to the toll-like receptor 4 (TLR4) agonist lipopolysaccharide (LPS); only two small patterns of LPS-induced genes were sensitive to GSH depletion. One group, mapping to innate immunity and antiviral responses (Oas2, Oas3, Mx2, Irf7, Irf9, STAT1, il1b), required GSH for optimal induction. Consequently, GSH depletion prevented the LPS-induced activation of antiviral response and its inhibition of influenza virus infection. LPS induction of a second group of genes (Prdx1, Srxn1, Hmox1, GSH synthase, cysteine transporters), mapping to nrf2 and the oxidative stress response, was increased by GSH depletion. We conclude that the main function of endogenous GSH is not to limit inflammation but to fine-tune the innate immune response to infection. |
|---|---|
| Language | English |
| Publishing date | 2017 |
| Publishing country | Switzerland |
| Document type | Journal Article |
| ZDB-ID | 2606827-8 |
| ISSN | 1664-3224 |
| ISSN | 1664-3224 |
| DOI | 10.3389/fimmu.2017.01239 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
More links
Kategorien
Order via subito
This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.