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  1. Article ; Online: TRP Channels Expression Profile in Human End-Stage Heart Failure.

    Dragún, Martin / Gažová, Andrea / Kyselovič, Ján / Hulman, Michal / Máťuš, Marek

    Medicina (Kaunas, Lithuania)

    2019  Volume 55, Issue 7

    Abstract: ... ...

    Abstract Objectives
    MeSH term(s) Analysis of Variance ; Female ; Heart Failure/blood ; Heart Failure/complications ; Heart Failure/physiopathology ; Heart Transplantation/adverse effects ; Heart Transplantation/methods ; Humans ; Male ; Middle Aged ; Protein-Serine-Threonine Kinases/analysis ; Protein-Serine-Threonine Kinases/blood ; Real-Time Polymerase Chain Reaction/methods ; Statistics, Nonparametric ; TRPC Cation Channels/analysis ; TRPC Cation Channels/blood ; TRPM Cation Channels/analysis ; TRPM Cation Channels/blood ; Transient Receptor Potential Channels/analysis ; Transient Receptor Potential Channels/blood ; Transient Receptor Potential Channels/pharmacology
    Chemical Substances TRPC Cation Channels ; TRPC5 protein, human ; TRPM Cation Channels ; TRPM4 protein, human ; Transient Receptor Potential Channels ; transient receptor potential cation channel, subfamily C, member 1 ; Protein-Serine-Threonine Kinases (EC 2.7.11.1) ; TRPM7 protein, human (EC 2.7.11.1)
    Language English
    Publishing date 2019-07-16
    Publishing country Switzerland
    Document type Journal Article
    ZDB-ID 2188113-3
    ISSN 1648-9144 ; 1010-660X
    ISSN (online) 1648-9144
    ISSN 1010-660X
    DOI 10.3390/medicina55070380
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: TRP Channels Expression Profile in Human End-Stage Heart Failure

    Martin Dragún / Andrea Gažová / Ján Kyselovič / Michal Hulman / Marek Máťuš

    Medicina, Vol 55, Iss 7, p

    2019  Volume 380

    Abstract: ... and MEF2c expression. Conclusions : The end-stage heart failure displays distinct expressional changes ... Here, we provide systematic analysis of TRP channels expression in human failing myocardium. Methods ... heart failure. The results highlight the complex interplay between TRP channels and the need for deeper analysis ...

    Abstract Objectives : Many studies indicate the involvement of transient receptor potential (TRP) channels in the development of heart hypertrophy. However, the data is often conflicted and has originated in animal models. Here, we provide systematic analysis of TRP channels expression in human failing myocardium. Methods and results : Left-ventricular tissue samples were isolated from explanted hearts of NYHA III-IV patients undergoing heart transplants (n = 43). Quantitative real-time PCR was performed to assess the mRNA levels of TRPC, TRPM and TRPV channels. Analysis of functional, clinical and biochemical data was used to confirm an end-stage heart failure diagnosis. Compared to myocardium samples from healthy donor hearts (n = 5), we detected a distinct increase in the expression of TRPC1, TRPC5, TRPM4 and TRPM7, and decreased expression of TRPC4 and TRPV2. These changes were not dependent on gender, clinical or biochemical parameters, nor functional parameters of the heart. We detected, however, a significant correlation of TRPC1 and MEF2c expression. Conclusions : The end-stage heart failure displays distinct expressional changes of TRP channels. Our findings provide a systematic description of TRP channel expression in human heart failure. The results highlight the complex interplay between TRP channels and the need for deeper analysis of early stages of hypertrophy and heart failure development.
    Keywords heart failure ; human ; TRP channels ; calcium signaling ; Medicine (General) ; R5-920
    Subject code 610
    Language English
    Publishing date 2019-07-01T00:00:00Z
    Publisher MDPI AG
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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