Article ; Online: TRPM7-mediated Ca2+ signals confer fibrogenesis in human atrial fibrillation.
2010 Volume 106, Issue 5, Page(s) 992–1003
Abstract: ... Transforming growth factor (TGF)-beta1, the major stimulator of atrial fibrosis, requires TRPM7-mediated Ca(2+) signal ... 2+) signals contribute to fibrogenesis in human AF.: Methods and results: We demonstrate ... permeable channel in human atrial fibroblasts. Endogenous TRPM7 currents in atrial fibroblasts resemble ...
| Abstract | Rationale: Cardiac fibrosis contributes to pathogenesis of atrial fibrillation (AF), which is the most commonly sustained arrhythmia and a major cause of morbidity and mortality. Although it has been suggested that Ca(2+) signals are involved in fibrosis promotion, the molecular basis of Ca(2+) signaling mechanisms and how Ca(2+) signals contribute to fibrogenesis remain unknown. Objective: To determine the molecular mechanisms of Ca(2+)-permeable channel(s) in human atrial fibroblasts, and to investigate how Ca(2+) signals contribute to fibrogenesis in human AF. Methods and results: We demonstrate that the transient receptor potential (TRP) melastatin related 7 (TRPM7) is the molecular basis of the major Ca(2+)-permeable channel in human atrial fibroblasts. Endogenous TRPM7 currents in atrial fibroblasts resemble the biophysical and pharmacological properties of heterologous expressed TRPM7. Knocking down TRPM7 by small hairpin RNA largely eliminates TRPM7 current and Ca(2+) influx in atrial fibroblasts. More importantly, atrial fibroblasts from AF patients show a striking upregulation of both TRPM7 currents and Ca(2+) influx and are more prone to myofibroblast differentiation, presumably attributable to the enhanced expression of TRPM7. TRPM7 small hairpin RNA markedly reduced basal AF fibroblast differentiation. Transforming growth factor (TGF)-beta1, the major stimulator of atrial fibrosis, requires TRPM7-mediated Ca(2+) signal for its effect on fibroblast proliferation and differentiation. Furthermore, TGF-beta1-induced differentiation of cultured human atrial fibroblasts is well correlated with an increase of TRPM7 expression induced by TGF-beta1. Conclusions: Our results establish that TRPM7 is the major Ca(2+)-permeable channel in human atrial fibroblasts and likely plays an essential role in TGF-beta1-elicited fibrogenesis in human AF. |
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| MeSH term(s) | Aged ; Aged, 80 and over ; Animals ; Atrial Fibrillation/metabolism ; Atrial Fibrillation/pathology ; Calcium Signaling ; Cell Differentiation ; Cell Membrane Permeability ; Cell Proliferation ; Female ; Fibroblasts/metabolism ; Fibroblasts/pathology ; Fibrosis ; Heart Atria/metabolism ; Heart Atria/pathology ; Humans ; Male ; Membrane Potentials ; Mice ; Protein-Serine-Threonine Kinases ; RNA Interference ; TRPM Cation Channels/genetics ; TRPM Cation Channels/metabolism ; Transforming Growth Factor beta1/metabolism ; Up-Regulation | ||||||||||
| Chemical Substances | TRPM Cation Channels ; Transforming Growth Factor beta1 ; Trpm7 protein, mouse (EC 2.7.1.-) ; Protein-Serine-Threonine Kinases (EC 2.7.11.1) ; TRPM7 protein, human (EC 2.7.11.1) | ||||||||||
| Language | English | ||||||||||
| Publishing date | 2010-01-14 | ||||||||||
| Publishing country | United States | ||||||||||
| Document type | Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't | ||||||||||
| ZDB-ID | 80100-8 | ||||||||||
| ISSN | 1524-4571 ; 0009-7330 ; 0931-6876 | ||||||||||
| ISSN (online) | 1524-4571 | ||||||||||
| ISSN | 0009-7330 ; 0931-6876 | ||||||||||
| DOI | 10.1161/CIRCRESAHA.109.206771 | ||||||||||
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| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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