Article ; Online: Angiotensin I-converting enzyme type 2 expression is increased in pancreatic islets of type 2 diabetic donors.
Diabetes/metabolism research and reviews
2023 Volume 39, Issue 8, Page(s) e3696
Abstract: Aims: Angiotensin I-converting enzyme type 2 (ACE2), a pivotal SARS-CoV-2 receptor, has been shown ... to be expressed in multiple cells, including human pancreatic beta-cells. A putative bidirectional ... However, whether ACE2 expression levels are altered in beta-cells of diabetic patients has not yet been investigated ...
| Abstract | Aims: Angiotensin I-converting enzyme type 2 (ACE2), a pivotal SARS-CoV-2 receptor, has been shown to be expressed in multiple cells, including human pancreatic beta-cells. A putative bidirectional relationship between SARS-CoV-2 infection and diabetes has been suggested, confirming the hypothesis that viral infection in beta-cells may lead to new-onset diabetes or worse glycometabolic control in diabetic patients. However, whether ACE2 expression levels are altered in beta-cells of diabetic patients has not yet been investigated. Here, we aimed to elucidate the in situ expression pattern of ACE2 in Type 2 diabetes (T2D) with respect to non-diabetic donors which may account for a higher susceptibility to SARS-CoV-2 infection in beta-cells. Material and methods: Angiotensin I-converting enzyme type 2 immunofluorescence analysis using two antibodies alongside insulin staining was performed on formalin-fixed paraffin embedded pancreatic sections obtained from n = 20 T2D and n = 20 non-diabetic (ND) multiorgan donors. Intensity and colocalisation analyses were performed on a total of 1082 pancreatic islets. Macrophage detection was performed using anti-CD68 immunohistochemistry on serial sections from the same donors. Results: Using two different antibodies, ACE2 expression was confirmed in beta-cells and in pancreas microvasculature. Angiotensin I-converting enzyme type 2 expression was increased in pancreatic islets of T2D donors in comparison to ND controls alongside with a higher colocalisation rate between ACE2 and insulin using both anti-ACE2 antibodies. CD68 Conclusions: Higher ACE2 expression in T2D islets might increase their susceptibility to SARS-CoV-2 infection during COVID-19 in T2D patients, thus worsening glycometabolic outcomes and disease severity. |
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| MeSH term(s) | Humans ; Angiotensin-Converting Enzyme 2 ; COVID-19/metabolism ; Diabetes Mellitus, Type 2/metabolism ; Insulin/metabolism ; Islets of Langerhans/metabolism ; Peptidyl-Dipeptidase A | |||||
| Chemical Substances | Angiotensin-Converting Enzyme 2 (EC 3.4.17.23) ; Insulin ; Peptidyl-Dipeptidase A (EC 3.4.15.1) ; ACE2 protein, human (EC 3.4.17.23) | |||||
| Language | English | |||||
| Publishing date | 2023-07-19 | |||||
| Publishing country | England | |||||
| Document type | Journal Article ; Research Support, Non-U.S. Gov't | |||||
| ZDB-ID | 1470192-3 | |||||
| ISSN | 1520-7560 ; 1520-7552 | |||||
| ISSN (online) | 1520-7560 | |||||
| ISSN | 1520-7552 | |||||
| DOI | 10.1002/dmrr.3696 | |||||
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| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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