Artikel ; Online: Serologic responses to SARS-CoV-2 infection among hospital staff with mild disease in eastern France.
2020 Band 59, Seite(n) 102915
Abstract: Background: The serologic response of individuals with mild forms of SARS-CoV-2 infection is ... 02).: Interpretation: Antibodies against SARS-CoV-2 were detected in virtually all hospital staff ... in the investigation, 160 reported SARS-CoV-2 infection that had not required hospital admission and were included ...
| Abstract | Background: The serologic response of individuals with mild forms of SARS-CoV-2 infection is poorly characterized. Methods: Hospital staff who had recovered from mild forms of PCR-confirmed SARS-CoV-2 infection were tested for anti-SARS-CoV-2 antibodies using two assays: a rapid immunodiagnostic test (99.4% specificity) and the S-Flow assay (~99% specificity). The neutralizing activity of the sera was tested with a pseudovirus-based assay. Findings: Of 162 hospital staff who participated in the investigation, 160 reported SARS-CoV-2 infection that had not required hospital admission and were included in these analyses. The median time from symptom onset to blood sample collection was 24 days (IQR: 21-28, range 13-39). The rapid immunodiagnostic test detected antibodies in 153 (95.6%) of the samples and the S-Flow assay in 159 (99.4%), failing to detect antibodies in one sample collected 18 days after symptom onset (the rapid test did not detect antibodies in that patient). Neutralizing antibodies (NAbs) were detected in 79%, 92% and 98% of samples collected 13-20, 21-27 and 28-41 days after symptom onset, respectively (P = 0.02). Interpretation: Antibodies against SARS-CoV-2 were detected in virtually all hospital staff sampled from 13 days after the onset of COVID-19 symptoms. This finding supports the use of serologic testing for the diagnosis of individuals who have recovered from SARS-CoV-2 infection. The neutralizing activity of the antibodies increased overtime. Future studies will help assess the persistence of the humoral response and its associated neutralization capacity in recovered patients. Fundings: The funders had no role in study design, data collection, interpretation, or the decision to submit the work for publication. |
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| Mesh-Begriff(e) | Adult ; Antibodies, Neutralizing/blood ; Antibodies, Viral/blood ; Betacoronavirus/genetics ; Betacoronavirus/isolation & purification ; COVID-19 ; Coronavirus Infections/diagnosis ; Coronavirus Infections/pathology ; Coronavirus Infections/virology ; Female ; France ; Health Personnel ; Hospitals ; Humans ; Male ; Middle Aged ; Pandemics ; Pneumonia, Viral/diagnosis ; Pneumonia, Viral/pathology ; Pneumonia, Viral/virology ; RNA, Viral/metabolism ; Reverse Transcriptase Polymerase Chain Reaction ; SARS-CoV-2 ; Serologic Tests ; Severity of Illness Index |
| Chemische Substanzen | Antibodies, Neutralizing ; Antibodies, Viral ; RNA, Viral |
| Schlagwörter | covid19 |
| Sprache | Englisch |
| Erscheinungsdatum | 2020-07-31 |
| Erscheinungsland | Netherlands |
| Dokumenttyp | Journal Article |
| ZDB-ID | 2851331-9 |
| ISSN | 2352-3964 |
| ISSN (online) | 2352-3964 |
| DOI | 10.1016/j.ebiom.2020.102915 |
| Datenquelle | MEDical Literature Analysis and Retrieval System OnLINE |
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