Article ; Online: Association of a reduction of G‑protein coupled receptor 30 expression and the pathogenesis of preeclampsia.
Molecular medicine reports
2017 Volume 16, Issue 5, Page(s) 5997–6003
Abstract: ... associated with pathogenesis of the disease during pregnancy. G‑protein‑coupled receptor 30 (GPR30) mediates ... Preeclampsia is a pregnancy‑specific disorder, which is a leading cause of maternal and ... in placentae from uncomplicated pregnancies and pregnancies complicated by preeclampsia using ...
| Abstract | Preeclampsia is a pregnancy‑specific disorder, which is a leading cause of maternal and perinatal mortality and morbidity. A lower increase of estrogen, compared with the increase in progesterone, is associated with pathogenesis of the disease during pregnancy. G‑protein‑coupled receptor 30 (GPR30) mediates the action of estrogen, however remains to be investigated in preeclampsia. The levels of GPR30 were measured in placentae from uncomplicated pregnancies and pregnancies complicated by preeclampsia using immunohistochemistry and western blotting. GPR30 expression was additionally measured in placental HTR8/SVneo cells following 17β‑estrogen (E2) treatment in normal or hypoxia‑reoxygenation conditions by western blotting. In addition, the outgrowth of HTR8/SVneo cells following E2 treatment in hypoxia‑reoxygenation conditions was measured. Levels of GPR30 were significantly reduced in placentae from women with preeclampsia as compared with uncomplicated pregnancies. Treatment with E2 significantly increased the expression of GPR30 in HTR8/SVneo cells, in normal and hypoxia‑reoxygenation conditions. Furthermore, treatment with E2 increased the outgrowth of HTR8/SVneo cells in hypoxia‑reoxygenation conditions. The present study demonstrated lowered placental expression of GPR30 in preeclampsia. Estrogen treatment increases GPR30 expression in extravillous trophoblast and GPR30 may be involved in extravillous trophoblast invasion. |
|---|---|
| MeSH term(s) | Adult ; Benzodioxoles/pharmacology ; Case-Control Studies ; Cell Hypoxia/genetics ; Cell Line ; Estradiol/pharmacology ; Female ; Gene Expression Regulation ; Humans ; Placenta/drug effects ; Placenta/metabolism ; Placenta/pathology ; Pre-Eclampsia/genetics ; Pre-Eclampsia/metabolism ; Pre-Eclampsia/pathology ; Pregnancy ; Quinolines/pharmacology ; Receptors, Estrogen/antagonists & inhibitors ; Receptors, Estrogen/genetics ; Receptors, Estrogen/metabolism ; Receptors, G-Protein-Coupled/agonists ; Receptors, G-Protein-Coupled/antagonists & inhibitors ; Receptors, G-Protein-Coupled/genetics ; Receptors, G-Protein-Coupled/metabolism ; Tissue Culture Techniques ; Trophoblasts/drug effects ; Trophoblasts/metabolism ; Trophoblasts/pathology |
| Chemical Substances | 4-(6-bromo-1,3-benzodioxol-5-yl)-3a,4,5,9b-3H-cyclopenta(c)quinoline ; Benzodioxoles ; GPER1 protein, human ; Quinolines ; Receptors, Estrogen ; Receptors, G-Protein-Coupled ; Estradiol (4TI98Z838E) |
| Language | English |
| Publishing date | 2017-08-23 |
| Publishing country | Greece |
| Document type | Journal Article |
| ISSN | 1791-3004 |
| ISSN (online) | 1791-3004 |
| DOI | 10.3892/mmr.2017.7341 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
More links
Kategorien
Order via subito
This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.
Inter-library loan at ZB MED
Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.