Article ; Online: Evaluation of nCoV-QS (MiCo BioMed) for RT-qPCR detection of SARS-CoV-2 from nasopharyngeal samples using CDC FDA EUA qPCR kit as a gold standard: An example of the need of validation studies.
2020 Volume 128, Page(s) 104454
Abstract: ... due to covid19 emergency.: Objective: We evaluated nCoV-QS (MiCo BioMed) kit using CDC kit as gold ... quality control probe.: Conclusions: Validation studies should be implemented for any SARS-CoV-2 RT-qPCR ... Background: Several qPCR kits are available for SARS-CoV-2 diagnosis, mostly lacking of evaluation ...
| Abstract | Background: Several qPCR kits are available for SARS-CoV-2 diagnosis, mostly lacking of evaluation due to covid19 emergency. Objective: We evaluated nCoV-QS (MiCo BioMed) kit using CDC kit as gold standard. Results: We found limitations for nCoV-QS: 1) lower sensitivity 2) lack of RNA quality control probe. Conclusions: Validation studies should be implemented for any SARS-CoV-2 RT-qPCR commercial kit to prevent unreliable diagnosis. |
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| MeSH term(s) | Betacoronavirus/genetics ; Betacoronavirus/isolation & purification ; COVID-19 ; Coronavirus Infections/diagnosis ; Coronavirus Infections/virology ; Humans ; Nasopharynx/virology ; Pandemics ; Pneumonia, Viral/diagnosis ; Pneumonia, Viral/virology ; Reagent Kits, Diagnostic ; Real-Time Polymerase Chain Reaction/methods ; SARS-CoV-2 ; Sensitivity and Specificity |
| Chemical Substances | Reagent Kits, Diagnostic |
| Keywords | covid19 |
| Language | English |
| Publishing date | 2020-05-22 |
| Publishing country | Netherlands |
| Document type | Journal Article |
| ZDB-ID | 1446080-4 |
| ISSN | 1873-5967 ; 1386-6532 |
| ISSN (online) | 1873-5967 |
| ISSN | 1386-6532 |
| DOI | 10.1016/j.jcv.2020.104454 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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