Article ; Online: Unified platform for genetic and serological detection of COVID-19 with single-molecule technology.
2021 Volume 16, Issue 7, Page(s) e0255096
Abstract: ... for a single-molecule based, enzyme-free assay for detection of SARS-CoV-2. The unified platform we developed ... stages of viral infection. The flexible and quantitative nature of single-molecule imaging technology ... as their immune response consisting of IgG and IgM antibodies. Thus, it establishes a platform for diagnostics of COVID-19 ...
| Abstract | The COVID-19 pandemic raises the need for diverse diagnostic approaches to rapidly detect different stages of viral infection. The flexible and quantitative nature of single-molecule imaging technology renders it optimal for development of new diagnostic tools. Here we present a proof-of-concept for a single-molecule based, enzyme-free assay for detection of SARS-CoV-2. The unified platform we developed allows direct detection of the viral genetic material from patients' samples, as well as their immune response consisting of IgG and IgM antibodies. Thus, it establishes a platform for diagnostics of COVID-19, which could also be adjusted to diagnose additional pathogens. |
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| MeSH term(s) | Antibodies, Viral/blood ; Base Sequence ; COVID-19/blood ; COVID-19/diagnosis ; COVID-19/immunology ; COVID-19/virology ; COVID-19 Nucleic Acid Testing/methods ; COVID-19 Nucleic Acid Testing/standards ; COVID-19 Serological Testing/methods ; COVID-19 Serological Testing/standards ; Enzyme-Linked Immunosorbent Assay ; Humans ; Immune Sera/chemistry ; Immunoglobulin G/blood ; Immunoglobulin M/blood ; Nasopharynx/virology ; Polyproteins/blood ; Polyproteins/genetics ; RNA, Viral/blood ; RNA, Viral/genetics ; SARS-CoV-2/genetics ; SARS-CoV-2/immunology ; Sensitivity and Specificity ; Single Molecule Imaging/instrumentation ; Single Molecule Imaging/methods ; Viral Proteins/blood ; Viral Proteins/genetics |
| Chemical Substances | Antibodies, Viral ; Immune Sera ; Immunoglobulin G ; Immunoglobulin M ; ORF1ab polyprotein, SARS-CoV-2 ; Polyproteins ; RNA, Viral ; Viral Proteins |
| Language | English |
| Publishing date | 2021-07-26 |
| Publishing country | United States |
| Document type | Journal Article ; Research Support, Non-U.S. Gov't |
| ZDB-ID | 2267670-3 |
| ISSN | 1932-6203 ; 1932-6203 |
| ISSN (online) | 1932-6203 |
| ISSN | 1932-6203 |
| DOI | 10.1371/journal.pone.0255096 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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