Article ; Online: FABP5 deficiency enhances susceptibility to H1N1 influenza A virus-induced lung inflammation.
American journal of physiology. Lung cellular and molecular physiology
2013 Volume 305, Issue 1, Page(s) L64–72
Abstract: ... FABP5 expression decreased following influenza A infection in WT lung tissues that corresponded ... The early inflammatory response to influenza A virus infection contributes to severe lung ... levels of H1N1-specific IgG antibodies. FABP5 deficiency greatly enhanced oxidative damage and ...
| Abstract | The early inflammatory response to influenza A virus infection contributes to severe lung disease and continues to pose a serious threat to human health. The mechanisms by which inflammatory cells invade the respiratory tract remain unclear. Uncontrolled inflammation and oxidative stress cause lung damage in response to influenza A infection. We have previously shown that the fatty acid binding protein 5 (FABP5) has anti-inflammatory properties. We speculate that, as a transporter of fatty acids, FABP5 plays an important protective role against oxidative damage to lipids during infection as well. Using FABP5-/- and wild-type (WT) mice infected with influenza A virus, we showed that FABP5-/- mice had increased cell infiltration of macrophages and neutrophils compared with WT mice. FABP5-/- mice presented lower viral burden but lost as much weight as WT mice. The adaptive immune response was also increased in FABP5-/- mice as illustrated by the accumulation of T and B cells in the lung tissues and increased levels of H1N1-specific IgG antibodies. FABP5 deficiency greatly enhanced oxidative damage and lipid peroxidation following influenza A infection and presented with sustained tissue inflammation. Interestingly, FABP5 expression decreased following influenza A infection in WT lung tissues that corresponded to a decrease in the anti-inflammatory molecule PPAR-γ activity. In conclusion, our results demonstrate a previously unknown contribution of FABP5 to influenza A virus pathogenesis by controlling excessive oxidative damage and inflammation. This property could be exploited for therapeutic purposes. |
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| MeSH term(s) | Adaptive Immunity ; Animals ; Blotting, Western ; Cells, Cultured ; Disease Susceptibility ; Enzyme-Linked Immunosorbent Assay ; Fatty Acid-Binding Proteins/physiology ; Fluorescent Antibody Technique ; Humans ; Immunoenzyme Techniques ; Immunoprecipitation ; Influenza A Virus, H1N1 Subtype/pathogenicity ; Influenza, Human/complications ; Influenza, Human/immunology ; Influenza, Human/virology ; Lipid Peroxidation ; Mice ; Mice, Inbred C57BL ; Mice, Knockout ; Neoplasm Proteins/physiology ; Oxidative Stress ; PPAR gamma/metabolism ; Pneumonia/etiology ; Pneumonia/metabolism ; Pneumonia/pathology ; RNA, Messenger/genetics ; Real-Time Polymerase Chain Reaction ; Reverse Transcriptase Polymerase Chain Reaction | ||||||||||
| Chemical Substances | Fabp5 protein, mouse ; Fatty Acid-Binding Proteins ; Neoplasm Proteins ; PPAR gamma ; RNA, Messenger | ||||||||||
| Language | English | ||||||||||
| Publishing date | 2013-04-26 | ||||||||||
| Publishing country | United States | ||||||||||
| Document type | Comparative Study ; Journal Article ; Research Support, Non-U.S. Gov't | ||||||||||
| ZDB-ID | 1013184-x | ||||||||||
| ISSN | 1522-1504 ; 1040-0605 | ||||||||||
| ISSN (online) | 1522-1504 | ||||||||||
| ISSN | 1040-0605 | ||||||||||
| DOI | 10.1152/ajplung.00276.2012 | ||||||||||
| Shelf mark |
|
||||||||||
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
More links
Kategorien
In stock of ZB MED Cologne/Königswinter
| Uc I Zs.89: Show issues | Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular ab Jg. 2022: Lesesaal (EG) |
|||
| Zs.A 2749: Show issues | Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (2.OG) ab Jg. 2022: Lesesaal (EG) |
Order via subito
This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.