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  1. Article ; Online: Electron microscopy of SARS-CoV-2: a challenging task.

    Goldsmith, Cynthia S / Miller, Sara E / Martines, Roosecelis B / Bullock, Hannah A / Zaki, Sherif R

    Lancet (London, England)

    2020  Volume 395, Issue 10238, Page(s) e99

    MeSH term(s) Betacoronavirus ; COVID-19 ; Coronavirus Infections ; Endothelial Cells ; Humans ; Microscopy, Electron ; Pandemics ; Pneumonia, Viral ; SARS Virus ; SARS-CoV-2
    Keywords covid19
    Language English
    Publishing date 2020-05-19
    Publishing country England
    Document type Letter ; Comment
    ZDB-ID 3306-6
    ISSN 1474-547X ; 0023-7507 ; 0140-6736
    ISSN (online) 1474-547X
    ISSN 0023-7507 ; 0140-6736
    DOI 10.1016/S0140-6736(20)31188-0
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  2. Article ; Online: Electron microscopy of SARS-CoV-2: a challenging task - Authors' reply.

    Varga, Zsuzsanna / Flammer, Andreas J / Steiger, Peter / Haberecker, Martina / Andermatt, Rea / Zinkernagel, Annelies / Mehra, Mandeep R / Scholkmann, Felix / Schüpbach, Reto / Ruschitzka, Frank / Moch, Holger

    Lancet (London, England)

    2020  Volume 395, Issue 10238, Page(s) e100

    MeSH term(s) Betacoronavirus ; COVID-19 ; Coronavirus Infections ; Humans ; Microscopy, Electron ; Pandemics ; Pneumonia, Viral ; SARS Virus ; SARS-CoV-2
    Keywords covid19
    Language English
    Publishing date 2020-05-19
    Publishing country England
    Document type Letter ; Comment
    ZDB-ID 3306-6
    ISSN 1474-547X ; 0023-7507 ; 0140-6736
    ISSN (online) 1474-547X
    ISSN 0023-7507 ; 0140-6736
    DOI 10.1016/S0140-6736(20)31185-5
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  4. Article: Electron microscopy of SARS-CoV-2: a challenging task - Authors' reply

    Varga, Zsuzsanna / Flammer, Andreas J / Steiger, Peter / Haberecker, Martina / Andermatt, Rea / Zinkernagel, Annelies / Mehra, Mandeep R / Scholkmann, Felix / Schüpbach, Reto / Ruschitzka, Frank / Moch, Holger

    Lancet

    Keywords covid19
    Publisher WHO
    Document type Article
    Note WHO #Covidence: #324619
    Database COVID19

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  5. Article ; Online: Detection and identification of coronaviruses in human tissues using electron microscopy.

    Bullock, Hannah A / Goldsmith, Cynthia S / Miller, Sara E

    Microscopy research and technique

    2022  Volume 85, Issue 7, Page(s) 2740–2747

    Abstract: ... The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has underscored how challenging the task of identifying ... of coronavirus particles in SARS-CoV-2 positive tissues continues to be a challenging task. This review provides ... rough endoplasmic reticulum, as coronavirus particles in SARS-CoV-2 positive patient tissue specimens. To counter ...

    Abstract The identification of viral particles within a tissue specimen requires specific knowledge of viral ultrastructure and replication, as well as a thorough familiarity with normal subcellular organelles. The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has underscored how challenging the task of identifying coronavirus by electron microscopy (EM) can be. Numerous articles have been published mischaracterizing common subcellular structures, including clathrin- or coatomer- coated vesicles, multivesicular bodies, and rough endoplasmic reticulum, as coronavirus particles in SARS-CoV-2 positive patient tissue specimens. To counter these misinterpretations, we describe the morphological features of coronaviruses that should be used to differentiate coronavirus particles from subcellular structures. Further, as many of the misidentifications of coronavirus particles have stemmed from attempts to attribute tissue damage to direct infection by SARS-CoV-2, we review articles describing ultrastructural changes observed in specimens from SARS-CoV-2-infected individuals that do not necessarily provide EM evidence of direct viral infection. Ultrastructural changes have been observed in respiratory, cardiac, kidney, and intestinal tissues, highlighting the widespread effects that SARS-CoV-2 infection may have on the body, whether through direct viral infection or mediated by SARS-CoV-2 infection-induced inflammatory and immune processes. HIGHLIGHTS: The identification of coronavirus particles in SARS-CoV-2 positive tissues continues to be a challenging task. This review provides examples of coronavirus ultrastructure to aid in the differentiation of the virus from common cellular structures.
    MeSH term(s) COVID-19 ; Humans ; Microscopy, Electron ; Pandemics ; SARS-CoV-2
    Language English
    Publishing date 2022-04-04
    Publishing country United States
    Document type Journal Article ; Review
    ZDB-ID 1099714-3
    ISSN 1097-0029 ; 1059-910X
    ISSN (online) 1097-0029
    ISSN 1059-910X
    DOI 10.1002/jemt.24115
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  6. Article ; Online: Electron microscopy of SARS-CoV-2

    Goldsmith, Cynthia S / Miller, Sara E / Martines, Roosecelis B / Bullock, Hannah A / Zaki, Sherif R

    The Lancet

    a challenging task

    2020  Volume 395, Issue 10238, Page(s) e99

    Keywords General Medicine ; covid19
    Language English
    Publisher Elsevier BV
    Publishing country us
    Document type Article ; Online
    ZDB-ID 3306-6
    ISSN 1474-547X ; 0023-7507 ; 0140-6736
    ISSN (online) 1474-547X
    ISSN 0023-7507 ; 0140-6736
    DOI 10.1016/s0140-6736(20)31188-0
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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    In stock of ZB MED Cologne/Königswinter

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  7. Article ; Online: Electron microscopy of SARS-CoV-2

    Varga, Zsuzsanna / Flammer, Andreas J / Steiger, Peter / Haberecker, Martina / Andermatt, Rea / Zinkernagel, Annelies / Mehra, Mandeep R / Scholkmann, Felix / Schüpbach, Reto / Ruschitzka, Frank / Moch, Holger

    The Lancet

    a challenging task – Authors' reply

    2020  Volume 395, Issue 10238, Page(s) e100

    Keywords General Medicine ; covid19
    Language English
    Publisher Elsevier BV
    Publishing country us
    Document type Article ; Online
    ZDB-ID 3306-6
    ISSN 1474-547X ; 0023-7507 ; 0140-6736
    ISSN (online) 1474-547X
    ISSN 0023-7507 ; 0140-6736
    DOI 10.1016/s0140-6736(20)31185-5
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Ua VI Zs.5: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 2021: Bestellungen von Artikeln über das Online-Bestellformular
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  8. Article ; Online: Electron microscopy of SARS-CoV-2

    Varga, Zsuzsanna / Flammer, Andreas J / Steiger, Peter / Haberecker, Martina / Andermatt, Rea / Zinkernagel, Annelies / Mehra, Mandeep R / Scholkmann, Felix / Schüpbach, Reto / Ruschitzka, Frank / Moch, Holger

    Varga, Zsuzsanna; Flammer, Andreas J; Steiger, Peter; Haberecker, Martina; Andermatt, Rea; Zinkernagel, Annelies; Mehra, Mandeep R; Scholkmann, Felix; Schüpbach, Reto; Ruschitzka, Frank; Moch, Holger (2020). Electron microscopy of SARS-CoV-2: a challenging task – Authors' reply. The Lancet, 395(10238):e100.

    a challenging task – Authors' reply

    2020  

    Keywords Institute of Intensive Care Medicine ; Institute of Pathology and Molecular Pathology ; Clinic for Infectious Diseases ; 610 Medicine & health ; General Medicine ; covid19
    Language English
    Publishing date 2020-05-01
    Publisher Elsevier
    Publishing country ch
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

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