Article ; Online: Low extracellular magnesium does not impair glucose-stimulated insulin secretion.
PloS one
2019 Volume 14, Issue 6, Page(s) e0217925
Abstract: ... of extracellular Mg2+ concentrations from 1.0 mM to 0.5 mM did not affect glucose-stimulated insulin secretion ... therefore, to investigate the acute effects of extracellular Mg2+ on glucose-stimulated insulin secretion in primary mouse ... glucose conditions compared to mock-transfected cells. Our data suggest that insulin secretion is not ...
| Abstract | There is an increasing amount of clinical evidence that hypomagnesemia (serum Mg2+ levels < 0.7 mmol/l) contributes to type 2 diabetes mellitus pathogenesis. Amongst other hypotheses, it has been suggested that Mg2+ deficiency affects insulin secretion. The aim of this study was, therefore, to investigate the acute effects of extracellular Mg2+ on glucose-stimulated insulin secretion in primary mouse islets of Langerhans and the rat insulinoma INS-1 cell line. Here we show that acute lowering of extracellular Mg2+ concentrations from 1.0 mM to 0.5 mM did not affect glucose-stimulated insulin secretion in islets or in insulin-secreting INS-1 cells. The expression of key genes in the insulin secretory pathway (e.g. Gck, Abcc8) was also unchanged in both experimental models. Knockdown of the most abundant Mg2+ channel Trpm7 by siRNAs in INS-1 cells resulted in a 3-fold increase in insulin secretion at stimulatory glucose conditions compared to mock-transfected cells. Our data suggest that insulin secretion is not affected by acute lowering of extracellular Mg2+ concentrations. |
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| MeSH term(s) | Animals ; Extracellular Space/chemistry ; Gene Expression Regulation/drug effects ; Glucose/pharmacology ; Hyperglycemia/pathology ; Insulin Secretion/drug effects ; Insulin-Secreting Cells/drug effects ; Insulin-Secreting Cells/metabolism ; Insulin-Secreting Cells/pathology ; Magnesium/pharmacology ; Mice, Inbred C57BL ; RNA, Messenger/genetics ; RNA, Messenger/metabolism ; Rats ; TRPM Cation Channels/metabolism |
| Chemical Substances | RNA, Messenger ; TRPM Cation Channels ; Trpm7 protein, mouse (EC 2.7.1.-) ; Magnesium (I38ZP9992A) ; Glucose (IY9XDZ35W2) |
| Language | English |
| Publishing date | 2019-06-04 |
| Publishing country | United States |
| Document type | Journal Article ; Research Support, Non-U.S. Gov't |
| ISSN | 1932-6203 |
| ISSN (online) | 1932-6203 |
| DOI | 10.1371/journal.pone.0217925 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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