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  1. Article ; Online: First environmental surveillance for the presence of SARS-CoV-2 RNA in wastewater and river water in Japan.

    Haramoto, Eiji / Malla, Bikash / Thakali, Ocean / Kitajima, Masaaki

    The Science of the total environment

    2020  Volume 737, Page(s) 140405

    Abstract: ... The present study aimed to assess the presence of SARS-CoV-2 RNA in wastewater and river water in Yamanashi ... adsorption-direct RNA extraction method. SARS-CoV-2 RNA was successfully detected in one of five ... before chlorination) wastewater samples and river water samples were collected five times ...

    Abstract Wastewater-based epidemiology is a powerful tool to understand the actual incidence of coronavirus disease 2019 (COVID-19) in a community because severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the etiological agent of COVID-19, can be shed in the feces of infected individuals regardless of their symptoms. The present study aimed to assess the presence of SARS-CoV-2 RNA in wastewater and river water in Yamanashi Prefecture, Japan, using four quantitative and two nested PCR assays. Influent and secondary-treated (before chlorination) wastewater samples and river water samples were collected five times from a wastewater treatment plant and three times from a river, respectively, between March 17 and May 7, 2020. The wastewater and river water samples (200-5000 mL) were processed by using two different methods: the electronegative membrane-vortex (EMV) method and the membrane adsorption-direct RNA extraction method. Based on the observed concentrations of indigenous pepper mild mottle virus RNA, the EMV method was found superior to the membrane adsorption-direct RNA extraction method. SARS-CoV-2 RNA was successfully detected in one of five secondary-treated wastewater samples with a concentration of 2.4 × 10
    MeSH term(s) Betacoronavirus ; COVID-19 ; Coronavirus Infections ; Environmental Monitoring ; Humans ; Japan ; Pandemics ; Pneumonia, Viral ; RNA ; Rivers ; SARS-CoV-2 ; Waste Water
    Chemical Substances Waste Water ; RNA (63231-63-0)
    Keywords covid19
    Language English
    Publishing date 2020-06-20
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 121506-1
    ISSN 1879-1026 ; 0048-9697
    ISSN (online) 1879-1026
    ISSN 0048-9697
    DOI 10.1016/j.scitotenv.2020.140405
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  2. Article ; Online: First environmental surveillance for the presence of SARS-CoV-2 RNA in wastewater and river water in Japan

    Haramoto, Eiji / Malla, Bikash / Thakali, Ocean / Kitajima, Masaaki

    Science of The Total Environment

    2020  Volume 737, Page(s) 140405

    Keywords Environmental Engineering ; Waste Management and Disposal ; Pollution ; Environmental Chemistry ; covid19
    Language English
    Publisher Elsevier BV
    Publishing country us
    Document type Article ; Online
    ZDB-ID 121506-1
    ISSN 1879-1026 ; 0048-9697
    ISSN (online) 1879-1026
    ISSN 0048-9697
    DOI 10.1016/j.scitotenv.2020.140405
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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    In stock of ZB MED Cologne/Königswinter

    Zs.A 949: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (1.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  3. Article ; Online: First environmental surveillance for the presence of SARS-CoV-2 RNA in wastewater and river water in Japan

    Haramoto, Eiji / Malla, Bikash / Thakali, Ocean / Kitajima, Masaaki

    medRxiv

    Abstract: ... in the community. This is the first study reporting the detection of SARS-CoV-2 RNA in wastewater in Japan. ... regardless of their symptoms. The present study aimed to assess the presence of SARS-CoV-2 RNA in wastewater ... and river water in Yamanashi Prefecture, Japan, using four quantitative and two nested PCR assays ...

    Abstract Wastewater-based epidemiology can be a powerful tool to understand the actual incidence of coronavirus disease 2019 (COVID-19) in a community because severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the etiological agent of COVID-19, can be shed in the feces of infected individuals regardless of their symptoms. The present study aimed to assess the presence of SARS-CoV-2 RNA in wastewater and river water in Yamanashi Prefecture, Japan, using four quantitative and two nested PCR assays. Influent and secondary-treated (before chlorination) wastewater samples and river water samples were collected five times from a wastewater treatment plant and three times from a river, respectively, between March 17 and May 7, 2020. The wastewater and river water samples (200-5,000 mL) were processed by using two different methods: the electronegative membrane-vortex (EMV) method and the membrane adsorption-direct RNA extraction method. Based on the observed concentrations of indigenous pepper mild mottle virus RNA, the EMV method was found superior to the membrane adsorption-direct RNA extraction method. SARS-CoV-2 RNA was successfully detected in one of five secondary-treated wastewater samples with a concentration of 2.4 × 10<sup>3</sup> copies/L by N_Sarbeco qPCR assay following the EMV method, whereas all the influent samples were tested negative for SARS-CoV-2 RNA. This result could be attributed to higher limit of detection for influent (4.0 × 10<sup>3</sup>-8.2 × 10<sup>4</sup> copies/L) with a lower filtration volume (200 mL) compared to that for secondary-treated wastewater (1.4 × 10<sup>2</sup>-2.5 × 10<sup>3</sup> copies/L) with a higher filtration volume of 5,000 mL. None of the river water samples tested positive for SARS-CoV-2 RNA. Comparison with the reported COVID-19 cases in Yamanashi Prefecture showed that SARS-CoV-2 RNA was detected in the secondary-treated wastewater sample when the cases peaked in the community. This is the first study reporting the detection of SARS-CoV-2 RNA in wastewater in Japan.
    Keywords covid19
    Language English
    Publishing date 2020-06-07
    Publisher Cold Spring Harbor Laboratory Press
    Document type Article ; Online
    DOI 10.1101/2020.06.04.20122747
    Database COVID19

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  4. Article ; Online: First environmental surveillance for the presence of SARS-CoV-2 RNA in wastewater and river water in Japan

    Haramoto, E. / Malla, B. / Thakali, O. / Kitajima, M.

    Abstract: ... regardless of their symptoms. The present study aimed to assess the presence of SARS-CoV-2 RNA in wastewater ... of the river water samples tested positive for SARS-CoV-2 RNA. Comparison with the reported COVID-19 cases ... in Yamanashi Prefecture showed that SARS-CoV-2 RNA was detected in the secondary-treated wastewater ...

    Abstract Wastewater-based epidemiology can be a powerful tool to understand the actual incidence of coronavirus disease 2019 (COVID-19) in a community because severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the etiological agent of COVID-19, can be shed in the feces of infected individuals regardless of their symptoms. The present study aimed to assess the presence of SARS-CoV-2 RNA in wastewater and river water in Yamanashi Prefecture, Japan, using four quantitative and two nested PCR assays. Influent and secondary-treated (before chlorination) wastewater samples and river water samples were collected five times from a wastewater treatment plant and three times from a river, respectively, between March 17 and May 7, 2020. The wastewater and river water samples (200-5,000 mL) were processed by using two different methods: the electronegative membrane-vortex (EMV) method and the membrane adsorption-direct RNA extraction method. Based on the observed concentrations of indigenous pepper mild mottle virus RNA, the EMV method was found superior to the membrane adsorption-direct RNA extraction method. SARS-CoV-2 RNA was successfully detected in one of five secondary-treated wastewater samples with a concentration of 2.4 x 103 copies/L by N_Sarbeco qPCR assay following the EMV method, whereas all the influent samples were tested negative for SARS-CoV-2 RNA. This result could be attributed to higher limit of detection for influent (4.0 x 103-8.2 x 104 copies/L) with a lower filtration volume (200 mL) compared to that for secondary-treated wastewater (1.4 x 102-2.5 x 103 copies/L) with a higher filtration volume of 5,000 mL. None of the river water samples tested positive for SARS-CoV-2 RNA. Comparison with the reported COVID-19 cases in Yamanashi Prefecture showed that SARS-CoV-2 RNA was detected in the secondary-treated wastewater sample when the cases peaked in the community. This is the first study reporting the detection of SARS-CoV-2 RNA in wastewater in Japan.
    Keywords covid19
    Publisher MedRxiv; WHO
    Document type Article ; Online
    DOI 10.1101/2020.06.04.20122747
    Database COVID19

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  5. Article ; Online: First environmental surveillance for the presence of SARS-CoV-2 RNA in wastewater and river water in Japan

    Haramoto, Eiji / Malla, Bikash / Thakali, Ocean / Kitajima, Masaaki

    Abstract: ... The present study aimed to assess the presence of SARS-CoV-2 RNA in wastewater and river water in Yamanashi ... of the river water samples tested positive for SARS-CoV-2 RNA Comparison with the reported COVID-19 cases ... in Yamanashi Prefecture showed that SARS-CoV-2 RNA was detected in the secondary-treated wastewater ...

    Abstract Wastewater-based epidemiology is a powerful tool to understand the actual incidence of coronavirus disease 2019 (COVID-19) in a community because severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the etiological agent of COVID-19, can be shed in the feces of infected individuals regardless of their symptoms. The present study aimed to assess the presence of SARS-CoV-2 RNA in wastewater and river water in Yamanashi Prefecture, Japan, using four quantitative and two nested PCR assays. Influent and secondary-treated (before chlorination) wastewater samples and river water samples were collected five times from a wastewater treatment plant and three times from a river, respectively, between March 17 and May 7, 2020. The wastewater and river water samples (200-5000 mL) were processed by using two different methods: the electronegative membrane-vortex (EMV) method and the membrane adsorption-direct RNA extraction method. Based on the observed concentrations of indigenous pepper mild mottle virus RNA, the EMV method was found superior to the membrane adsorption-direct RNA extraction method. SARS-CoV-2 RNA was successfully detected in one of five secondary-treated wastewater samples with a concentration of 2.4 x 10(3) copies/L by N_Sarbeco qPCR assay following the EMV method, with sequence confirmation of the qPCR product, whereas all the influent samples were tested negative for SARS-CoV-2 RNA. This result could be attributed to higher limit of detection for influent (4.0 x 10(3)-82 x 10(4) copies L) with a lower filtration volume (200 mL) compared to that for secondary-treated wastewater (1.4 x 10(2)-2.5 x 10(3) copies/L) with a higher filtration volume of 5000 mL. None of the river water samples tested positive for SARS-CoV-2 RNA Comparison with the reported COVID-19 cases in Yamanashi Prefecture showed that SARS-CoV-2 RNA was detected in the secondary-treated wastewater sample when the cases peaked in the community. This is the first study reporting the detection of SARS-CoV-2 RNA in wastewater in Japan. (C) 2020 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
    Keywords COVID-19 ; River water ; SARS-CoV-2 ; Wastewater ; Wastewater-based epidemiology ; 518 ; covid19
    Subject code 500
    Language English
    Publisher Elsevier
    Publishing country jp
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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