Article ; Online: Human parvovirus B19 VP1u Protein as inflammatory mediators induces liver injury in naïve mice.
2016 Volume 7, Issue 2, Page(s) 110–118
Abstract: ... This study investigated the effect and possible inflammatory signaling of B19-VP1u in livers from BALB/c mice ... were observed in livers from BALB/c mice receiving COS-7 cells expressing B19-VP1u as well ... in the pathogenesis of B19V infection, the influence of B19-VP1u proteins on hepatic injury is still obscure ...
| Abstract | Human parvovirus B19 (B19V) is a human pathogen known to be associated with many non-erythroid diseases, including hepatitis. Although B19V VP1-unique region (B19-VP1u) has crucial roles in the pathogenesis of B19V infection, the influence of B19-VP1u proteins on hepatic injury is still obscure. This study investigated the effect and possible inflammatory signaling of B19-VP1u in livers from BALB/c mice that were subcutaneously inoculated with VP1u-expressing COS-7 cells. The in vivo effects of B19-VP1u were analyzed by using live animal imaging system (IVIS), Haematoxylin-Eosin staining, gel zymography, and immunoblotting after inoculation. Markedly hepatocyte disarray and lymphocyte infiltration, enhanced matrix metalloproteinase (MMP)-9 activity and increased phosphorylation of p38, ERK, IKK-α, IκB and NF-κB (p-p65) proteins were observed in livers from BALB/c mice receiving COS-7 cells expressing B19-VP1u as well as the significantly increased CRP, IL-1β and IL-6. Notably, IFN-γ and phosphorylated STAT1, but not STAT3, were also significantly increased in the livers of BALB/c mice that were subcutaneously inoculated with VP1u-expressing COS-7 cells. These findings revealed the effects of B19-VP1u on liver injury and suggested that B19-VP1u may have a role as mediators of inflammation in B19V infection. |
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| MeSH term(s) | Animals ; COS Cells ; Capsid Proteins/genetics ; Capsid Proteins/immunology ; Capsid Proteins/metabolism ; Cercopithecus aethiops ; Hepatitis, Viral, Animal/immunology ; Hepatitis, Viral, Animal/pathology ; Hepatitis, Viral, Animal/virology ; Inflammation Mediators/metabolism ; Interleukin-1beta/genetics ; Interleukin-1beta/metabolism ; Interleukin-6/genetics ; Interleukin-6/metabolism ; Intravital Microscopy ; Liver/immunology ; Liver/metabolism ; Liver/pathology ; Mice ; Mice, Inbred BALB C ; Parvovirus B19, Human/metabolism ; Parvovirus B19, Human/pathogenicity ; Phosphorylation ; Transfection |
| Chemical Substances | Capsid Proteins ; IL1B protein, mouse ; Inflammation Mediators ; Interleukin-1beta ; Interleukin-6 ; capsid protein VP1, parvovirus B19 ; interleukin-6, mouse |
| Language | English |
| Publishing date | 2016 |
| Publishing country | United States |
| Document type | Journal Article ; Research Support, Non-U.S. Gov't |
| ZDB-ID | 2657572-3 |
| ISSN | 2150-5608 ; 2150-5594 |
| ISSN (online) | 2150-5608 |
| ISSN | 2150-5594 |
| DOI | 10.1080/21505594.2015.1122165 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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