Article ; Online: Protective effects of recombinant human granulocyte macrophage colony stimulating factor on H1N1 influenza virus-induced pneumonia in mice.
2010 Volume 51, Issue 2, Page(s) 151–157
Abstract: Protective effects of recombinant human granulocyte macrophage colony stimulating factor (rHuGM-CSF ... on H1N1 influenza virus infection was studied in vivo and in vitro. Mice were infected with H1N1 ... suggested that rHuGM-CSF might be an effective and potential protection for H1N1 influenza virus-induced ...
| Abstract | Protective effects of recombinant human granulocyte macrophage colony stimulating factor (rHuGM-CSF) on H1N1 influenza virus infection was studied in vivo and in vitro. Mice were infected with H1N1 influenza A viruses and rHuGM-CSF at doses of 0.34, 0.67, and 1.34mgkg(-1)d(-1) was administrated for 7days before the mice were infected with influenza virus and continued for a further 3days. Compared with control mice, rHuGM-CSF was demonstrated to increase the survival rate of the infected mice by 50.0%, 55.6%, and 80.0% and increased the mean survival days by 25.7%, 30.0%, and 46.8%, respectively. Histopathological study of the lungs in pneumonia mice found that pre-treatment with rHuGM-CSF significantly ameliorated lung injury induced by influenza virus infection. In vitro study demonstrated that when rHuGM-CSF were co-incubated with peripheral blood mononuclear cells (PBMCs), the PBMCs culture supernatant induced a dose-dependent reduction of virus-induced cytopathic effect (CPE) in Madin-Darby canine kidney (MDCK) cells in vitro. These results suggested that rHuGM-CSF might be an effective and potential protection for H1N1 influenza virus-induced pneumonia. |
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| MeSH term(s) | Animals ; Female ; Granulocyte-Macrophage Colony-Stimulating Factor/therapeutic use ; Humans ; Influenza A Virus, H1N1 Subtype ; Leukocytes, Mononuclear/virology ; Lung/pathology ; Lung/virology ; Male ; Mice ; Mice, Inbred BALB C ; Orthomyxoviridae Infections/drug therapy ; Orthomyxoviridae Infections/mortality ; Orthomyxoviridae Infections/pathology ; Pneumonia, Viral/drug therapy ; Pneumonia, Viral/mortality ; Pneumonia, Viral/pathology ; Recombinant Proteins | |||||
| Chemical Substances | Recombinant Proteins ; Granulocyte-Macrophage Colony-Stimulating Factor (83869-56-1) | |||||
| Language | English | |||||
| Publishing date | 2010-08 | |||||
| Publishing country | England | |||||
| Document type | Journal Article ; Research Support, Non-U.S. Gov't | |||||
| ZDB-ID | 1018055-2 | |||||
| ISSN | 1096-0023 ; 1043-4666 | |||||
| ISSN (online) | 1096-0023 | |||||
| ISSN | 1043-4666 | |||||
| DOI | 10.1016/j.cyto.2010.04.001 | |||||
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| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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