Article ; Online: Stability of SARS-CoV-2 Spike G614 Variant Surpasses That of the D614 Variant after Cold Storage.
mSphere
2021 Volume 6, Issue 2
Abstract: ... stability after cold storage remain unclear. To compare the infectivity of two SARS-CoV-2 variants, namely ... than that of the S-D614 variant after storage at -20°C for up to 30 days. Our findings revealed that SARS-CoV-2 ... SARS-CoV-2 variants with spike protein with the D614 mutation (S-D614) and G614 mutation (S-G614 ...
| Abstract | Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) carrying the D614G mutation on the spike protein is the predominant circulating variant and is associated with enhanced infectivity. However, whether this dominant variant can potentially spread through the cold chain and whether the spike protein affects virus stability after cold storage remain unclear. To compare the infectivity of two SARS-CoV-2 variants, namely, SARS-CoV-2 variants with spike protein with the D614 mutation (S-D614) and G614 mutation (S-G614), after different periods of refrigeration (4°C) and freezing (-20°C). We also determined the integrity of the viral RNA and the ability of the spike protein to bind angiotensin-converting enzyme 2 (ACE2) after storage at these conditions. The results showed that SARS-CoV-2 was more stable and infectious after storage at -20°C than at 4°C. Particularly, the S-G614 variant was found to be more stable than the S-D614 variant. The spike protein of the S-G614 variant had better binding ability with the ACE2 receptor than that of the S-D614 variant after storage at -20°C for up to 30 days. Our findings revealed that SARS-CoV-2 remains stable and infectious after refrigeration or freezing, and their stability and infectivity up to 30 days depends on the spike variant. Stability and infectivity are related to each other, and the higher stability of S-G614 compared to that of S-D614 may contribute to rapid viral spread of the S-G614 variant. |
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| MeSH term(s) | COVID-19 ; Cold Temperature ; Genetic Fitness/genetics ; Humans ; Mutation ; Protein Stability ; SARS-CoV-2/genetics ; Spike Glycoprotein, Coronavirus/genetics |
| Chemical Substances | Spike Glycoprotein, Coronavirus ; spike protein, SARS-CoV-2 |
| Language | English |
| Publishing date | 2021-03-31 |
| Publishing country | United States |
| Document type | Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't |
| ISSN | 2379-5042 |
| ISSN (online) | 2379-5042 |
| DOI | 10.1128/mSphere.00104-21 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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