Article ; Online: Discovery of an Active RAG Transposon Illuminates the Origins of V(D)J Recombination.
2016 Volume 166, Issue 1, Page(s) 102–114
Abstract: Co-option of RAG1 and RAG2 for antigen receptor gene assembly by V(D)J recombination was a crucial ... target site duplications and a pair of terminal inverted repeats (TIRs) resembling V(D)J recombination ... that ProtoRAG represents a molecular "living fossil" of the long-sought RAG transposon. ...
| Abstract | Co-option of RAG1 and RAG2 for antigen receptor gene assembly by V(D)J recombination was a crucial event in the evolution of jawed vertebrate adaptive immunity. RAG1/2 are proposed to have arisen from a transposable element, but definitive evidence for this is lacking. Here, we report the discovery of ProtoRAG, a DNA transposon family from lancelets, the most basal extant chordates. A typical ProtoRAG is flanked by 5-bp target site duplications and a pair of terminal inverted repeats (TIRs) resembling V(D)J recombination signal sequences. Between the TIRs reside tail-to-tail-oriented, intron-containing RAG1-like and RAG2-like genes. We demonstrate that ProtoRAG was recently active in the lancelet germline and that the lancelet RAG1/2-like proteins can mediate TIR-dependent transposon excision, host DNA recombination, transposition, and low-efficiency TIR rejoining using reaction mechanisms similar to those used by vertebrate RAGs. We propose that ProtoRAG represents a molecular "living fossil" of the long-sought RAG transposon. |
|---|---|
| MeSH term(s) | Animals ; DNA Transposable Elements ; DNA-Binding Proteins ; Evolution, Molecular ; Homeodomain Proteins ; Lancelets/genetics ; Terminal Repeat Sequences ; V(D)J Recombination |
| Chemical Substances | DNA Transposable Elements ; DNA-Binding Proteins ; Homeodomain Proteins ; V(D)J recombination activating protein 2 ; RAG-1 protein (128559-51-3) |
| Language | English |
| Publishing date | 2016-06-09 |
| Publishing country | United States |
| Document type | Journal Article ; Research Support, N.I.H., Extramural ; Research Support, Non-U.S. Gov't |
| ZDB-ID | 187009-9 |
| ISSN | 1097-4172 ; 0092-8674 |
| ISSN (online) | 1097-4172 |
| ISSN | 0092-8674 |
| DOI | 10.1016/j.cell.2016.05.032 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
Full text online
More links
Kategorien
In stock of ZB MED Cologne/Königswinter
| Zs.A 1167: Show issues | Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG) |
|||
| Zs.MG 58: Show issues |
Order via subito
This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.