Article: Regulated expression of CD36 during monocyte-to-macrophage differentiation: potential role of CD36 in foam cell formation.
1996 Volume 87, Issue 5, Page(s) 2020–2028
Abstract: ... Thus, the transient expression of CD36 during monocyte-to-macrophage differentiation, and the ability of M-CSF ... a transient eightfold increase during monocyte-to-macrophage differentiation, peaking at the early macrophage ... apoptotic neutrophils. In this study, we examined the expression of CD36 during in vitro differentiation ...
| Abstract | CD36 is an 88-kD integral membrane glycoprotein expressed on monocytes, platelets, and certain microvascular endothelium serving distinct cellular functions both as an adhesive receptor for thrombospondin, collagen, and Plasmodium falciparum-infected erythrocytes, and as a scavenger receptor for oxidized low-density lipoprotein and apoptotic neutrophils. In this study, we examined the expression of CD36 during in vitro differentiation of peripheral blood monocytes into culture-derived macrophages. Steady-state mRNA levels of CD36 showed a transient eightfold increase during monocyte-to-macrophage differentiation, peaking at the early macrophage stage (days 3 or 4 in culture), following a gradual decrease back to baseline levels by the mature macrophage stage (days 7 or 8 in culture). Immunoblotting with monoclonal antibodies to CD36 supported this transient, yet significant (8- to 10-fold) increase in total protein levels of CD36. The increased CD36 protein was observed at the plasma membrane, whereas an intracellular pool of CD36 was also detected from day 2 to day 6 in culture through indirect immunofluorescence. A concomitant twofold increase in the cells' ability to bind 125I-thrombospondin at the early macrophage stage (day 4) verified the functional competency of the plasma membrane localized CD36, and supported the presence of an intracellular pool of CD36. The in vitro differentiated macrophages as well as alveolar macrophages remained responsive to macrophage colony-stimulating factor (M-CSF), a known transcriptional regulator of monocyte CD36. The M-CSF-induced macrophages resulted in enhanced foam cell formation, which was inhibitable with monoclonal antibodies to CD36. Thus, the transient expression of CD36 during monocyte-to-macrophage differentiation, and the ability of M-CSF to maintain macrophage CD36 at elevated levels, may serve as a critical process in dictating the functional activity of CD36 during inflammatory responses and atherogenesis. |
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| MeSH term(s) | Animals ; CD36 Antigens/biosynthesis ; CD36 Antigens/genetics ; CD36 Antigens/physiology ; Cell Differentiation/drug effects ; Cell Membrane/metabolism ; Cells, Cultured ; Foam Cells/cytology ; Gene Expression Regulation/drug effects ; Humans ; Intracellular Fluid/metabolism ; Lipoproteins, LDL/metabolism ; Macrophage Colony-Stimulating Factor/pharmacology ; Macrophages/cytology ; Macrophages/drug effects ; Macrophages, Alveolar/drug effects ; Macrophages, Alveolar/metabolism ; Mice ; Monocytes/cytology ; Monocytes/drug effects ; Monocytes/metabolism ; RNA, Messenger/biosynthesis | ||||||||||
| Chemical Substances | CD36 Antigens ; Lipoproteins, LDL ; RNA, Messenger ; Macrophage Colony-Stimulating Factor (81627-83-0) | ||||||||||
| Language | English | ||||||||||
| Publishing date | 1996-03-01 | ||||||||||
| Publishing country | United States | ||||||||||
| Document type | Journal Article ; Research Support, U.S. Gov't, P.H.S. | ||||||||||
| ZDB-ID | 80069-7 | ||||||||||
| ISSN | 1528-0020 ; 0006-4971 | ||||||||||
| ISSN (online) | 1528-0020 | ||||||||||
| ISSN | 0006-4971 | ||||||||||
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| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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