Article: Application of hybrid LRR technique to protein crystallization.
2008 Volume 41, Issue 5, Page(s) 353–357
Abstract: ... their production and crystallization, we have invented a novel method termed "Hybrid LRR Technique". Using ... design principles and application of the technique to protein crystallization will be summarized ... hybridization did not disturb structures and functions of the target TLR proteins. The Hybrid LRR Technique is ...
| Abstract | LRR family proteins play important roles in a variety of physiological processes. To facilitate their production and crystallization, we have invented a novel method termed "Hybrid LRR Technique". Using this technique, the first crystal structures of three TLR family proteins could be determined. In this review, design principles and application of the technique to protein crystallization will be summarized. For crystallization of TLRs, hagfish VLR receptors were chosen as the fusion partners and the TLR and the VLR fragments were fused at the conserved LxxLxLxxN motif to minimize local structural incompatibility. TLR-VLR hybridization did not disturb structures and functions of the target TLR proteins. The Hybrid LRR Technique is a general technique that can be applied to structural studies of other LRR proteins. It may also have broader application in biochemical and medical application of LRR proteins by modifying them without compromising their structural integrity. |
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| MeSH term(s) | Animals ; Crystallization/methods ; Humans ; Models, Biological ; Protein Structure, Tertiary ; Proteins/chemistry ; Proteins/genetics ; Proteins/metabolism ; Recombinant Proteins/chemistry ; Recombinant Proteins/metabolism ; Toll-Like Receptors/chemistry ; Toll-Like Receptors/genetics ; Toll-Like Receptors/metabolism | |||||
| Chemical Substances | Proteins ; Recombinant Proteins ; Toll-Like Receptors ; leucine-rich repeat proteins | |||||
| Language | English | |||||
| Publishing date | 2008-05-03 | |||||
| Publishing country | Korea (South) | |||||
| Document type | Journal Article ; Research Support, Non-U.S. Gov't ; Review | |||||
| ZDB-ID | 2410389-5 | |||||
| ISSN | 1976-670X ; 1976-6696 | |||||
| ISSN (online) | 1976-670X | |||||
| ISSN | 1976-6696 | |||||
| DOI | 10.5483/bmbrep.2008.41.5.353 | |||||
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| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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