Article: Detection and Isolation of SARS-CoV-2 in Serum, Urine, and Stool Specimens of COVID-19 Patients from the Republic of Korea.
Osong public health and research perspectives
2020 Volume 11, Issue 3, Page(s) 112–117
Abstract: ... Of the samples collected from 74 COVID-19 patients, SARS-CoV-2 was detected in 15 serum, urine, or stool samples ... and likelihood of SARS-CoV-2 transmission from COVID-19 patients through non-respiratory routes ... patients for several days after hospitalization, its detection in the serum, urine, and stool samples was ...
| Abstract | Objectives: Coronavirus Disease-19 (COVID-19) is a respiratory infection characterized by the main symptoms of pneumonia and fever. It is caused by the novel coronavirus severe acute respiratory syndrome Coronavirus-2 (SARS-CoV-2), which is known to spread via respiratory droplets. We aimed to determine the rate and likelihood of SARS-CoV-2 transmission from COVID-19 patients through non-respiratory routes. Methods: Serum, urine, and stool samples were collected from 74 hospitalized patients diagnosed with COVID-19 based on the detection of SARS-CoV-2 in respiratory samples. The SARS-CoV-2 RNA genome was extracted from each specimen and real-time reverse transcription polymerase chain reaction performed. CaCo-2 cells were inoculated with the specimens containing the SARS-COV-2 genome, and subcultured for virus isolation. After culturing, viral replication in the cell supernatant was assessed. Results: Of the samples collected from 74 COVID-19 patients, SARS-CoV-2 was detected in 15 serum, urine, or stool samples. The virus detection rate in the serum, urine, and stool samples were 2.8% (9/323), 0.8% (2/247), and 10.1% (13/129), and the mean viral load was 1,210 ± 1,861, 79 ± 30, and 3,176 ± 7,208 copy/μL, respectively. However, the SARS-CoV-2 was not isolated by the culture method from the samples that tested positive for the SARS-CoV-2 gene. Conclusion: While the virus remained detectable in the respiratory samples of COVID-19 patients for several days after hospitalization, its detection in the serum, urine, and stool samples was intermittent. Since the virus could not be isolated from the SARS-COV-2-positive samples, the risk of viral transmission via stool and urine is expected to be low. |
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| Keywords | covid19 |
| Language | English |
| Publishing date | 2020-05-28 |
| Publishing country | Korea (South) |
| Document type | Journal Article |
| ZDB-ID | 2656431-2 |
| ISSN | 2233-6052 ; 2210-9099 |
| ISSN (online) | 2233-6052 |
| ISSN | 2210-9099 |
| DOI | 10.24171/j.phrp.2020.11.3.02 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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