Article ; Online: Aspects of "antigen-antibody" interaction of chicken infectious bronchitis virus determined by surface plasmon resonance.
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy
2021 Volume 264, Page(s) 120236
Abstract: Authors performed investigation on "antigen-antibody" interaction of chicken infectious bronchitis ... coronavirus (IBV) by a method based on the surface plasmon resonance (SPR). Presence of space-size effect ... of functionalization of sensor's sensitive element surface, as well as acidity of buffer solution on the activity of antigen ...
| Abstract | Authors performed investigation on "antigen-antibody" interaction of chicken infectious bronchitis coronavirus (IBV) by a method based on the surface plasmon resonance (SPR). Presence of space-size effect related to a difference between antigen and antibody particle sizes has been theoretically grounded and experimentally proven. Herewith, the difference between responses of the SPR-sensor to specific and non-specific interactions is considerably less (up to 6.3 times) than the expected one (8 - 11 times). An impact of functionalization of sensor's sensitive element surface, as well as acidity of buffer solution on the activity of antigen-antibody interaction was studied here. The difference between sensor's responses to specific and non-specific interactions increased two-fold from 200 to 432ang sec due to this treatment. When changing the acidity of analyzed solution from pH7.3 to pH6.8, the corresponding difference between sensor's responses increased by 6.3 times from 194 up to 1235ang.sec. Thus, an impact of space-size effect on interaction between IBV antigen and specific antibody can be considerably (almost in 3 times) decreased by reducing the acidity of used buffer solution. The results of our investigation can be successfully applied to develop new methods for detection of pathogens and specific antibodies using SPR. |
|||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| MeSH term(s) | Animals ; Antibodies ; Chickens ; Coronavirus Infections/veterinary ; Infectious bronchitis virus ; Surface Plasmon Resonance | |||||||||||||||
| Chemical Substances | Antibodies | |||||||||||||||
| Language | English | |||||||||||||||
| Publishing date | 2021-07-29 | |||||||||||||||
| Publishing country | England | |||||||||||||||
| Document type | Journal Article | |||||||||||||||
| ZDB-ID | 210413-1 | |||||||||||||||
| ISSN | 1873-3557 ; 0370-8322 ; 0584-8539 ; 1386-1425 | |||||||||||||||
| ISSN (online) | 1873-3557 | |||||||||||||||
| ISSN | 0370-8322 ; 0584-8539 ; 1386-1425 | |||||||||||||||
| DOI | 10.1016/j.saa.2021.120236 | |||||||||||||||
| Shelf mark |
|
|||||||||||||||
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
Full text online
More links
Kategorien
In stock of ZB MED Bonn / Germany
| Z 51/98: Show issues | ||||
| Z 1611 a: Show issues | ||||
| 924/340: Show issues |
Order via subito
This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.
Inter-library loan at ZB MED
Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.