Artikel: Pathogenicity of influenza A virus in ependymal organ culture.
1981 Band 24, Heft 2, Seite(n) 201–213
Abstract: Ependymal organ culture was used as a model to study the effect of influenza A virus ... of microvilli and cilia was decreased in influenza A virus-infected explants. At 7 days, the ependymal cells ... with 0.1 ml of an inoculum containing 35 plaque-forming units (PFU) of neurotropic influenza A virus (WSN ...
| Abstract | Ependymal organ culture was used as a model to study the effect of influenza A virus on the ciliated ependyma of the rat. One-square-milli-meter portions of cerebellum from newborn rats were harvested in roller tubes containing Ham's F-12 medium calf serum, and glutamine. Ciliary activity was monitored by stereomicroscopy and, after vigorous ciliary motion was established, tubes were inoculated with 0.1 ml of an inoculum containing 35 plaque-forming units (PFU) of neurotropic influenza A virus (WSN strain). Explants were examined by scanning electron microscopy (SEM) at 2 and 7 days and by transmission electron microscopy (TEM) at 7 days. When compared to noninfected controls, SEM showed that at 2 days the density of microvilli and cilia was decreased in influenza A virus-infected explants. At 7 days, the ependymal cells were nearly denuded of cilia and microvilli, and macrophage like cells were frequently resting upon the ependymal surface. TEM showed numerous viral particles, both budding from the cells surface and located extracellularly near the cell surface. |
|||||
|---|---|---|---|---|---|---|
| Mesh-Begriff(e) | Animals ; Cilia/ultrastructure ; Ependyma/ultrastructure ; Influenza A virus/growth & development ; Microscopy, Electron ; Microscopy, Electron, Scanning ; Organ Culture Techniques ; Rats ; Time Factors ; Virus Cultivation | |||||
| Sprache | Englisch | |||||
| Erscheinungsdatum | 1981-10 | |||||
| Erscheinungsland | United States | |||||
| Dokumenttyp | Journal Article | |||||
| ZDB-ID | 123029-3 | |||||
| ISSN | 1096-9926 ; 0040-3709 | |||||
| ISSN (online) | 1096-9926 | |||||
| ISSN | 0040-3709 | |||||
| DOI | 10.1002/tera.1420240211 | |||||
| Signatur |
|
|||||
| Datenquelle | MEDical Literature Analysis and Retrieval System OnLINE |
Zusatzmaterialien
Kategorien
Verfügbar in ZB MED Köln/Königswinter
| Zs.A 749: Hefte anzeigen | Standort: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG) |
Über subito bestellen
Dieser Service ist kostenpflichtig (siehe Lieferbedingungen von subito). Bestellungen, die einen Artikel nebst Supplementary Material umfassen, werden grundsätzlich wie mehrfache Bestellungen bearbeitet. Gebühren fallen in diesen Fällen für jede einzelne Bestellung an.