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  1. Article ; Online: Update on serologic testing in COVID-19.

    Krajewski, Rafał / Gołębiowska, Justyna / Makuch, Sebastian / Mazur, Grzegorz / Agrawal, Siddarth

    Clinica chimica acta; international journal of clinical chemistry

    2020  Volume 510, Page(s) 746–750

    Abstract: Serologic tests are one of the available diagnostic tools in COVID-19. Growing literature ... algorithms that include serological tests in patients with confirmed or suspected COVID-19. ... we discuss the utility of anti-SARS-CoV-2 serology testing in a clinical setting and propose diagnostic ...

    Abstract Serologic tests are one of the available diagnostic tools in COVID-19. Growing literature highlights their role in the clinical management of the disease. Unfortunately, due to the limited availability of commercial tests and the lack of reliable trials establishing the sensitivity and specificity of the diagnostic method, the clinical application of the test needs to be precisely determined. In this paper, we discuss the utility of anti-SARS-CoV-2 serology testing in a clinical setting and propose diagnostic algorithms that include serological tests in patients with confirmed or suspected COVID-19.
    MeSH term(s) Antibodies, Viral/blood ; COVID-19 ; Coronavirus Infections/blood ; Coronavirus Infections/diagnosis ; Coronavirus Infections/immunology ; Humans ; Pandemics ; Pneumonia, Viral/blood ; Pneumonia, Viral/diagnosis ; Pneumonia, Viral/immunology ; Serologic Tests
    Chemical Substances Antibodies, Viral
    Keywords covid19
    Language English
    Publishing date 2020-09-15
    Publishing country Netherlands
    Document type Journal Article ; Review
    ZDB-ID 80228-1
    ISSN 1873-3492 ; 0009-8981
    ISSN (online) 1873-3492
    ISSN 0009-8981
    DOI 10.1016/j.cca.2020.09.015
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  2. Article ; Online: Update to COVID-19 serologic testing : FAQs and caveats.

    Kadkhoda, Kamran

    Cleveland Clinic journal of medicine

    2022  

    Language English
    Publishing date 2022-01-12
    Publishing country United States
    Document type Journal Article
    ZDB-ID 639116-3
    ISSN 1939-2869 ; 0891-1150
    ISSN (online) 1939-2869
    ISSN 0891-1150
    DOI 10.3949/ccjm.87a.20054-up
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  3. Article ; Online: Update on serologic testing in COVID–19

    Krajewski, Rafał / Gołębiowska, Justyna / Makuch, Sebastian / Mazur, Grzegorz / Agrawal, Siddarth

    Clinica Chimica Acta

    2020  Volume 510, Page(s) 746–750

    Keywords Clinical Biochemistry ; Biochemistry ; Biochemistry, medical ; General Medicine ; covid19
    Language English
    Publisher Elsevier BV
    Publishing country us
    Document type Article ; Online
    ZDB-ID 80228-1
    ISSN 1873-3492 ; 0009-8981
    ISSN (online) 1873-3492
    ISSN 0009-8981
    DOI 10.1016/j.cca.2020.09.015
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  4. Article: Update on serologic testing in COVID-19

    Krajewski, Rafal / Golebiowska, Justyna / Makuch, Sebastian / Mazur, Grzegorz / Agrawal, Siddarth

    Clin Chim Acta

    Abstract: Serologic tests are one of the available diagnostic tools in COVID-19. Growing literature ... algorithms that include serological tests in patients with confirmed or suspected COVID-19. ... we discuss the utility of anti-SARS-CoV-2 serology testing in a clinical setting and propose diagnostic ...

    Abstract Serologic tests are one of the available diagnostic tools in COVID-19. Growing literature highlights their role in the clinical management of the disease. Unfortunately, due to the limited availability of commercial tests and the lack of reliable trials establishing the sensitivity and specificity of the diagnostic method, the clinical application of the test needs to be precisely determined. In this paper, we discuss the utility of anti-SARS-CoV-2 serology testing in a clinical setting and propose diagnostic algorithms that include serological tests in patients with confirmed or suspected COVID-19.
    Keywords covid19
    Publisher WHO
    Document type Article
    Note WHO #Covidence: #758644
    Database COVID19

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  5. Article ; Online: An Update on the Recent Diagnostic Modalities of SARS-CoV-2

    Marie Victor Pravin Charles / Arunava Kali / Santhanam Vidyalakshmi

    Journal of Clinical and Diagnostic Research, Vol 17, Iss 3, Pp DE01-DE

    A Review

    2023  Volume 06

    Abstract: The pandemic of respiratory viral pneumonia initiated as an outbreak during December 2019, at the Hubei region, China. The demanding research work conducted by the scientific community across the globe identified the causative agent as Severe Acute ... ...

    Abstract The pandemic of respiratory viral pneumonia initiated as an outbreak during December 2019, at the Hubei region, China. The demanding research work conducted by the scientific community across the globe identified the causative agent as Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). The Case Fatality Rate (CFR) is high for the infection. Laboratory diagnosis of infectious diseases has an integral role in case surveillance, diagnosis, treatment, and prevention. SARS-CoV-2 is a positive sense single stranded Ribonucleic Acid (RNA) virus grouped under the order nidovirales, Family Coronaviridae, and subfamily Orthocoronavirinae. The structural proteins are spike protein (S), membrane protein (M), nucleocapsid protein (N), and the envelope protein (E). The common symptoms are fever, cough, dyspnoea, fatigue, headache, muscle pain, anosmia, and ageusia. The less common symptoms are sore throat, congestion, nausea, vomiting and diarrhoea. Sample collection is a critical step in the laboratory diagnosis of SARS-CoV-2 infection. The molecular methods used for diagnosis of SARS-CoV-2 are Real time Reverse Transcription Polymerase Chain Reaction (r-RT-PCR), Reverse Transcription Loop Mediated Isothermal Amplification (RT-LAMP), digital PCR, Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) based assay and sequencing methods. The antigen and antibody detection methods supplement the molecular tests. Although a huge array of tests are available, the pertinent choice of diagnostic test is crucial. The molecular detection methods are highly sensitive, accurate and reliable.
    Keywords covid-19 diagnostic testing ; covid-19 nucleic acid testing ; sars-cov-2 infection serological testing ; Medicine ; R
    Subject code 572 ; 570
    Language English
    Publishing date 2023-03-01T00:00:00Z
    Publisher JCDR Research and Publications Private Limited
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  6. Article ; Online: Serological and Molecular Tests for COVID-19: a recent update.

    Mansourabadi, Amir Hossein / Sadeghalvad, Mona / Mohammadi-Motlagh, Hamid-Reza / Amirzargar, Aliakbar

    Iranian journal of immunology : IJI

    2021  Volume 18, Issue 1, Page(s) 13–33

    Abstract: The COVID-19 pandemic is probably the most devastating worldwide challenge in recent century. COVID ... the onset of symptoms for both IgM and IgA and 10-18 days for IgG. Following the outbreak of COVID-19, FDA ... of the viral infection. Although the serological tests may not play an important role in the active case of COVID-19 ...

    Abstract The COVID-19 pandemic is probably the most devastating worldwide challenge in recent century. COVID-19 leads to a mild to severe respiratory disease and affects different organs and has become a global concern since December 2019. Meanwhile, molecular biology and diagnostic laboratories played an essential role in diagnosis of the disease by introducing serological and molecular tests. Molecular-based techniques are reliable detection tools for SARS-CoV-2 and used for diagnosis of patients especially in the early stage of the disease. While, serological assays are considered as additional tools to verify the asymptomatic infections, tracing previous contacts of individuals, vaccine efficacy, and study the seroprevalance. The average time of the appearance of anti-SARS-CoV-2 antibodies in the patient's serum is 3-6 days after the onset of symptoms for both IgM and IgA and 10-18 days for IgG. Following the outbreak of COVID-19, FDA has approved and authorized a series of serological laboratory tests for early diagnosis. Serological assays have low-cost and provide fast results but have poor sensitivity in the early stage of the viral infection. Although the serological tests may not play an important role in the active case of COVID-19, it could be effective to determine the immunity of health care workers, and confirm late COVID-19 cases during the outbreak. In this review, we compared various laboratory diagnostic assays for COVID-19.
    MeSH term(s) Antibodies, Viral/blood ; Biomarkers/blood ; COVID-19/blood ; COVID-19/diagnosis ; COVID-19/genetics ; COVID-19/immunology ; COVID-19 Nucleic Acid Testing ; COVID-19 Serological Testing ; Early Diagnosis ; Host-Pathogen Interactions ; Humans ; Predictive Value of Tests ; RNA, Viral/blood ; RNA, Viral/genetics ; Reproducibility of Results ; SARS-CoV-2/genetics ; SARS-CoV-2/immunology
    Chemical Substances Antibodies, Viral ; Biomarkers ; RNA, Viral
    Language English
    Publishing date 2021-04-14
    Publishing country Iran
    Document type Journal Article ; Review
    ISSN 1735-367X
    ISSN (online) 1735-367X
    DOI 10.22034/iji.2021.88660.1894
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  7. Article ; Online: Clinical Pathway for Early Diagnosis of COVID-19: Updates from Experience to Evidence-Based Practice.

    Xu, Guogang / Yang, Yongshi / Du, Yingzhen / Peng, Fujun / Hu, Peng / Wang, Runsheng / Yin, Ming / Li, Tianzhi / Tu, Lei / Sun, Jinlyu / Jiang, Taijiao / Chang, Christopher

    Clinical reviews in allergy & immunology

    2020  Volume 59, Issue 1, Page(s) 89–100

    Abstract: The COVID-19 pandemic is a significant global event in the history of infectious diseases. The SARS ... through droplet or direct contact. Clinical features of COVID-19 include high fever, cough, and fatigue ...

    Abstract The COVID-19 pandemic is a significant global event in the history of infectious diseases. The SARS-CoV-2 appears to have originated from bats but is now easily transmissible among humans, primarily through droplet or direct contact. Clinical features of COVID-19 include high fever, cough, and fatigue which may progress to ARDS. Respiratory failure can occur rapidly after this. The primary laboratory findings include lymphopenia and eosinopenia. Elevated D-dimer, procalcitonin, and CRP levels may correlate with disease severity. Imaging findings include ground-glass opacities and patchy consolidation on CT scan. Mortality is higher in patients with hypertension, cardiac disease, diabetes mellitus, cancer, and COPD. Elderly patients are more susceptible to severe disease and death, while children seem to have lower rates of infection and lower mortality. Diagnostic criteria and the identification of persons under investigation have evolved as more data has emerged. However, the approach to diagnosis is still very variable from region to region, country to country, and even among different hospitals in the same city. The importance of a clinical pathway to implement the most effective and relevant diagnostic strategy is of critical importance to establish the control of this virus that is responsible for more and more deaths each day.
    MeSH term(s) Algorithms ; Antibodies, Viral/immunology ; Betacoronavirus/immunology ; COVID-19 ; COVID-19 Testing ; COVID-19 Vaccines ; Clinical Laboratory Techniques/methods ; Coronavirus Infections/diagnosis ; Critical Pathways ; Early Diagnosis ; Evidence-Based Practice ; False Negative Reactions ; Humans ; Immunoglobulin G/immunology ; Immunoglobulin M/immunology ; Lung/diagnostic imaging ; Medical History Taking ; Pandemics ; Patient Isolation ; Pneumonia, Viral/diagnosis ; Quarantine ; RNA, Viral/analysis ; Real-Time Polymerase Chain Reaction/methods ; Reverse Transcriptase Polymerase Chain Reaction/methods ; SARS-CoV-2 ; Serologic Tests/methods ; Severity of Illness Index ; Tomography, X-Ray Computed
    Chemical Substances Antibodies, Viral ; COVID-19 Vaccines ; Covid-19 aAPC vaccine ; Immunoglobulin G ; Immunoglobulin M ; RNA, Viral
    Keywords covid19
    Language English
    Publishing date 2020-04-23
    Publishing country United States
    Document type Journal Article ; Review
    ZDB-ID 1239045-8
    ISSN 1559-0267 ; 1080-0549
    ISSN (online) 1559-0267
    ISSN 1080-0549
    DOI 10.1007/s12016-020-08792-8
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