Article ; Online: Development of qPCR platform with probes for quantifying prevalent and biomedically relevant human gut microbial taxa.
2020 Volume 52, Page(s) 101570
Abstract: ... analysis for 12 prokaryotic taxa that are prevalent in the human gut and associated with diseases, diet ... for assessing human gut microbiome composition. The system allows conducting qualitative and semiquantitative ... applications. Here, we designed a qPCR-based platform with fluorescent-labeled oligonucleotide probes ...
Abstract | Nowadays the advent of innovative high-throughput sequencing allows obtaining high-quality microbiome profiling. However, PCR-based tests are still considered the "golden standard" for many clinical applications. Here, we designed a qPCR-based platform with fluorescent-labeled oligonucleotide probes for assessing human gut microbiome composition. The system allows conducting qualitative and semiquantitative analysis for 12 prokaryotic taxa that are prevalent in the human gut and associated with diseases, diet, age and other factors. The platform was validated by comparing microbiome profile data obtained with two different methods - the platform and high-throughput 16S rRNA sequencing - across 42 stool samples. The test can form the basis for precise and cost-efficient microbiome assay for large-scale surveys including clinical trials with interventions related to diet and disease risks. |
|||||
---|---|---|---|---|---|---|
Keywords | covid19 | |||||
Language | English | |||||
Publishing date | 2020-04-15 | |||||
Publishing country | England | |||||
Document type | Journal Article ; Research Support, Non-U.S. Gov't | |||||
ZDB-ID | 639082-1 | |||||
ISSN | 1096-1194 ; 0890-8508 | |||||
ISSN (online) | 1096-1194 | |||||
ISSN | 0890-8508 | |||||
DOI | 10.1016/j.mcp.2020.101570 | |||||
Shelf mark |
|
|||||
Database | MEDical Literature Analysis and Retrieval System OnLINE |
Full text online
More links
Kategorien
In stock of ZB MED Cologne/Königswinter
Zs.A 2230: Show issues | Location: Je nach Verfügbarkeit (siehe Angabe bei Bestand) bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular Jg. 1995 - 2021: Lesesall (1.OG) ab Jg. 2022: Lesesaal (EG) |
Order via subito
This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.