Article ; Online: Alteration of the transcriptional profile of human embryonic kidney cells by transient overexpression of mouse TRPM7 channels.
2011 Volume 27, Issue 3-4, Page(s) 313–326
Abstract: ... on cellular gene expression, inducible HEK293 cell-lines harboring the wild-type mouse TRPM7 and a mutant ... lacking the kinase domain were established. The wild-type and the non-functional TRPM7 channels were ... in transcription significantly and specifically by the expression of the functional TRPM7 channel.: Conclusion ...
| Abstract | Background: TRPM7 is a cation channel containing a functional kinase domain. The functional activity of TRPM7 is essential for cell viability and growth, and its expression is up-regulated in certain pathological conditions, such as ischemia. Methods: In order to assess the effects of TRPM7 activity on cellular gene expression, inducible HEK293 cell-lines harboring the wild-type mouse TRPM7 and a mutant lacking the kinase domain were established. The wild-type and the non-functional TRPM7 channels were induced transiently and the comparative changes in cellular transcription were investigated. Results: From the genome-scale analysis using a human genome expression microarray, we identified 951 genes altered in transcription significantly and specifically by the expression of the functional TRPM7 channel. Conclusion: By analyzing the genes differentially expressed by TRPM7, we were able to provide potential target proteins and to delineate the cellular pathways affected by the channel function. |
|---|---|
| MeSH term(s) | Animals ; Gene Expression Profiling ; Genome, Human ; HEK293 Cells ; Humans ; Mice ; Microarray Analysis ; TRPM Cation Channels/genetics ; TRPM Cation Channels/metabolism ; Transfection |
| Chemical Substances | TRPM Cation Channels ; Trpm7 protein, mouse (EC 2.7.1.-) |
| Language | English |
| Publishing date | 2011-04-01 |
| Publishing country | Germany |
| Document type | Journal Article ; Research Support, Non-U.S. Gov't |
| ZDB-ID | 1067572-3 |
| ISSN | 1421-9778 ; 1015-8987 |
| ISSN (online) | 1421-9778 |
| ISSN | 1015-8987 |
| DOI | 10.1159/000327958 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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