Article ; Online: Inhibition of Transient Receptor Potential Melastain 7 Enhances Apoptosis Induced by TRAIL in PC-3 cells.
Asian Pacific journal of cancer prevention : APJCP
2015 Volume 16, Issue 10, Page(s) 4469–4475
Abstract: ... In this study, we demonstrated the influence and potential function of TRPM7 on the PC-3 cells apoptosis induced ... Transient receptor potential melastain 7 (TRPM7) is a bifunctional protein with dual structure ... but also increased the apoptosis of TRAIL-treated PC-3 cells, which may be regulated ...
| Abstract | Transient receptor potential melastain 7 (TRPM7) is a bifunctional protein with dual structure of both ion channel and protein kinase, participating in a wide variety of diseases including cancer. Recent researches have reported the mechanism of TRPM7 in human cancers. However, the correlation between TRPM7 and prostate cancer (PCa) has not been well studied. The objective of this study was to investigate the potential the role of TRPM7 in the apoptosis of PC-3 cells, which is the key cell of advanced metastatic PCa. In this study, we demonstrated the influence and potential function of TRPM7 on the PC-3 cells apoptosis induced by TNF-related apoptosis inducing-ligand (TRAIL). The study also found a novel up-regulated expression of TRPM7 in PC-3 cells after treating with TRAIL. Suppression of TRPM7 by TRPM7 non-specific inhibitors (Gd3+ or 2-aminoethoxy diphenylborate (2-APB) ) not only markedly eliminated TRPM7 expression level, but also increased the apoptosis of TRAIL-treated PC-3 cells, which may be regulated by the phosphatidylinositol 3-kinase/protein kinase B (PI3K/AKT) signaling pathway accompany with up-regulated expression of cleaved Caspase-3, (TRAIL-receptor 1, death receptors 4) DR4, and (TRAIL-receptor 2, death receptors 5) DR5. Taken together, our findings strongly suggested that TRPM7 was involved in the apoptosis of PC-3 cells induced by TRAIL, indicating that TRPM7 may be applied as a therapeutic target for PCa. |
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| MeSH term(s) | Apoptosis/drug effects ; Boron Compounds/pharmacology ; Cell Line, Tumor ; Gadolinium/pharmacology ; Humans ; Male ; Phosphatidylinositol 3-Kinases/metabolism ; Prostatic Neoplasms/enzymology ; Prostatic Neoplasms/metabolism ; Protein-Serine-Threonine Kinases/antagonists & inhibitors ; Protein-Serine-Threonine Kinases/genetics ; Protein-Serine-Threonine Kinases/metabolism ; Proto-Oncogene Proteins c-akt/metabolism ; RNA, Messenger/metabolism ; RNA, Small Interfering/pharmacology ; Signal Transduction ; TNF-Related Apoptosis-Inducing Ligand/pharmacology ; TRPM Cation Channels/antagonists & inhibitors ; TRPM Cation Channels/genetics ; TRPM Cation Channels/metabolism ; Up-Regulation/drug effects |
| Chemical Substances | Boron Compounds ; RNA, Messenger ; RNA, Small Interfering ; TNF-Related Apoptosis-Inducing Ligand ; TRPM Cation Channels ; Gadolinium (AU0V1LM3JT) ; 2-aminoethoxydiphenyl borate (E4ES684O93) ; Phosphatidylinositol 3-Kinases (EC 2.7.1.-) ; Protein-Serine-Threonine Kinases (EC 2.7.11.1) ; Proto-Oncogene Proteins c-akt (EC 2.7.11.1) ; TRPM7 protein, human (EC 2.7.11.1) |
| Language | English |
| Publishing date | 2015-05-27 |
| Publishing country | Thailand |
| Document type | Journal Article ; Research Support, Non-U.S. Gov't |
| ZDB-ID | 2218955-5 |
| ISSN | 2476-762X ; 1513-7368 |
| ISSN (online) | 2476-762X |
| ISSN | 1513-7368 |
| DOI | 10.7314/apjcp.2015.16.10.4469 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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