LIVIVO - The Search Portal for Life Sciences

zur deutschen Oberfläche wechseln
Advanced search

Search results

Result 1 - 6 of total 6

Search options

  1. Article ; Online: Promising methods for detection of novel coronavirus SARS-CoV-2.

    Liu, Ran / Fu, Aisi / Deng, Zixin / Li, Yan / Liu, Tiangang

    View (Beijing, China)

    2020  Volume 1, Issue 1, Page(s) e4

    Abstract: ... system-based technology, which can also be deployed for the detection of SARS-CoV-2. We further ... A very recent outbreak of the novel coronavirus, COVID-19, in the city of Wuhan, China, in December ... In this report, we summarized the six promising methods, including whole-genome sequencing, real-time ...

    Abstract A very recent outbreak of the novel coronavirus, COVID-19, in the city of Wuhan, China, in December 2019 and its subsequent spread within and across China have resulted in several deaths and infections. Presently, nucleic acid amplification test is essential for the confirmation of COVID infection. In this report, we summarized the six promising methods, including whole-genome sequencing, real-time reverse transcription polymerase chain reaction, nanopore target sequencing, antibody-based immunoassay techniques, use of paper-based biomolecular sensors, and the clustered regularly interspaced short palindromic repeats-Cas system-based technology, which can also be deployed for the detection of SARS-CoV-2. We further introduced the principles of these methods, discussed the scope and practicability of application of the available products and methods, and highlighted the potential approaches to develop additional products and techniques for early diagnosis of COVID-19.
    Language English
    Publishing date 2020-03-23
    Publishing country China
    Document type Journal Article
    ISSN 2688-268X
    ISSN (online) 2688-268X
    DOI 10.1002/viw2.4
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  2. Article ; Online: Promising methods for detection of novel coronavirus SARS‐CoV‐2

    Liu, Ran / Fu, Aisi / Deng, Zixin / Li, Yan / Liu, Tiangang

    View

    2020  Volume 1, Issue 1

    Keywords covid19
    Language English
    Publisher Wiley
    Publishing country us
    Document type Article ; Online
    ISSN 2688-268X
    DOI 10.1002/viw2.4
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

    Full text online

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  3. Article: Promising methods for detection of novel coronavirus SARS-CoV-2

    Liu, Ran / Fu, Aisi / Deng, Zixin / Li, Yan / Liu, Tiangang

    View

    Abstract: ... system-based technology, which can also be deployed for the detection of SARS-CoV-2 We further introduced ... Abstract A very recent outbreak of the novel coronavirus, COVID-19, in the city of Wuhan, China ... In this report, we summarized the six promising methods, including whole-genome sequencing, real-time ...

    Abstract Abstract A very recent outbreak of the novel coronavirus, COVID-19, in the city of Wuhan, China, in December 2019 and its subsequent spread within and across China have resulted in several deaths and infections Presently, nucleic acid amplification test is essential for the confirmation of COVID infection In this report, we summarized the six promising methods, including whole-genome sequencing, real-time reverse transcription polymerase chain reaction, nanopore target sequencing, antibody-based immunoassay techniques, use of paper-based biomolecular sensors, and the clustered regularly interspaced short palindromic repeats-Cas system-based technology, which can also be deployed for the detection of SARS-CoV-2 We further introduced the principles of these methods, discussed the scope and practicability of application of the available products and methods, and highlighted the potential approaches to develop additional products and techniques for early diagnosis of COVID-19
    Keywords covid19
    Publisher WHO
    Document type Article
    Note WHO #Covidence: #11776
    Database COVID19

    Kategorien

    Inter-library loan at ZB MED

    Your chosen title can be delivered directly to ZB MED Cologne location if you are registered as a user at ZB MED Cologne.

  4. Article ; Online: Nanobody in a Double "Y"-Shaped Assembly: A Promising Candidate for Lateral Flow Immunoassays.

    Xiao, Yumeng / Dong, Hongjie / Wu, Cancan / Zhang, Kundi / Jiang, Xiaoqiong / Chen, Junyu / Wang, Hongwei / Xu, Sujuan / Zhang, Fengyu / Gu, Lichuan

    Analytical chemistry

    2024  Volume 96, Issue 18, Page(s) 7130–7137

    Abstract: ... the severe acute respiratory syndrome coronavirus-2 N protein, with a detection limit of at least 500 pg/mL. This study helps broaden ... regarding the exclusive use of Nbs in lateral flow immunoassay (LFIA) detection. Herein, we present a novel ... assays. Despite offering considerable advantages in detection applications, knowledge is limited ...

    Abstract Derived from camelid heavy-chain antibodies, nanobodies (Nbs) are the smallest natural antibodies and are an ideal tool in biological studies because of their simple structure, high yield, and low cost. Nbs possess significant potential for developing highly specific and user-friendly diagnostic assays. Despite offering considerable advantages in detection applications, knowledge is limited regarding the exclusive use of Nbs in lateral flow immunoassay (LFIA) detection. Herein, we present a novel double "Y" architecture, achieved by using the SpyTag/SpyCatcher and Im7/CL7 systems. The double "Y" assemblies exhibited a significantly higher affinity for their epitopes, as particularly evident in the reduced dissociation rate. An LFIA employing double "Y" assemblies was effectively used to detect the severe acute respiratory syndrome coronavirus-2 N protein, with a detection limit of at least 500 pg/mL. This study helps broaden the array of tools available for the development of Nb-based diagnostic techniques.
    MeSH term(s) Single-Domain Antibodies/chemistry ; Single-Domain Antibodies/immunology ; Immunoassay/methods ; SARS-CoV-2/immunology ; SARS-CoV-2/isolation & purification ; Limit of Detection ; Humans ; COVID-19/diagnosis ; COVID-19/virology ; COVID-19/immunology ; Coronavirus Nucleocapsid Proteins/immunology ; Coronavirus Nucleocapsid Proteins/analysis
    Chemical Substances Single-Domain Antibodies ; Coronavirus Nucleocapsid Proteins
    Language English
    Publishing date 2024-04-28
    Publishing country United States
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 1508-8
    ISSN 1520-6882 ; 0003-2700
    ISSN (online) 1520-6882
    ISSN 0003-2700
    DOI 10.1021/acs.analchem.4c00509
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 4033: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  5. Article ; Online: Nanomedicine as a promising strategy for the theranostics of infectious diseases.

    Liu, Hengyu / Zhong, Wenhao / Zhang, Xinyu / Lin, Dongjun / Wu, Jun

    Journal of materials chemistry. B

    2021  Volume 9, Issue 38, Page(s) 7878–7908

    Abstract: ... of the recent outbreak of coronavirus disease (COVID-19) have reached 110.7 million cases and over 2.4 million ... detection methods, the emergence of drug-resistant bacteria, serious side effects, and poor drug delivery ... methods and drug treatment efficacy. Owing to their unique optical, magnetic, and electrical properties ...

    Abstract Infectious diseases caused by bacteria, viruses, and fungi and their global spread pose a great threat to human health. The 2019 World Health Organization report predicted that infection-related mortality will be similar to cancer mortality by 2050. Particularly, the global cumulative numbers of the recent outbreak of coronavirus disease (COVID-19) have reached 110.7 million cases and over 2.4 million deaths as of February 23, 2021. Moreover, the crisis of these infectious diseases exposes the many problems of traditional diagnosis, treatment, and prevention, such as time-consuming and unselective detection methods, the emergence of drug-resistant bacteria, serious side effects, and poor drug delivery. There is an urgent need for rapid and sensitive diagnosis as well as high efficacy and low toxicity treatments. The emergence of nanomedicine has provided a promising strategy to greatly enhance detection methods and drug treatment efficacy. Owing to their unique optical, magnetic, and electrical properties, nanoparticles (NPs) have great potential for the fast and selective detection of bacteria, viruses, and fungi. NPs exhibit remarkable antibacterial activity by releasing reactive oxygen species and metal ions, exerting photothermal effects, and causing destruction of the cell membrane. Nano-based delivery systems can further improve drug permeability, reduce the side effects of drugs, and prolong systemic circulation time and drug half-life. Moreover, effective drugs against COVID-19 are still lacking. Recently, nanomedicine has shown great potential to accelerate the development of safe and novel anti-COVID-19 drugs. This article reviews the fundamental mechanisms and the latest developments in the treatment and diagnosis of bacteria, viruses, and fungi and discusses the challenges and perspectives in the application of nanomedicine.
    MeSH term(s) Anti-Infective Agents/chemistry ; Anti-Infective Agents/therapeutic use ; COVID-19/diagnosis ; COVID-19/drug therapy ; COVID-19/virology ; Communicable Diseases/diagnosis ; Communicable Diseases/drug therapy ; Communicable Diseases/microbiology ; Communicable Diseases/virology ; Drug Carriers/chemistry ; Humans ; Nanomedicine ; Nanoparticles/chemistry ; Reactive Oxygen Species/metabolism ; SARS-CoV-2/isolation & purification
    Chemical Substances Anti-Infective Agents ; Drug Carriers ; Reactive Oxygen Species
    Language English
    Publishing date 2021-10-06
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 2702241-9
    ISSN 2050-7518 ; 2050-750X
    ISSN (online) 2050-7518
    ISSN 2050-750X
    DOI 10.1039/d1tb01316e
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

  6. Article ; Online: An overview of molecular biology and nanotechnology based analytical methods for the detection of SARS-CoV-2: promising biotools for the rapid diagnosis of COVID-19.

    Kailasa, Suresh Kumar / Mehta, Vaibhavkumar N / Koduru, Janardhan Reddy / Basu, Hirakendu / Singhal, Rakesh Kumar / Murthy, Z V P / Park, Tae-Jung

    The Analyst

    2021  Volume 146, Issue 5, Page(s) 1489–1513

    Abstract: ... of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2; 2019-nCoV) play an essential role in the diagnosis of COVID-19 ... Currently, the 2019 novel coronavirus (2019-nCoV) is drastically affecting 214 countries, causing ... molecular biology and nanotechnology based analytical methods have proven to be promising diagnostic tools ...

    Abstract Currently, the 2019 novel coronavirus (2019-nCoV) is drastically affecting 214 countries, causing severe pneumonia in patients, which has resulted in lockdown being implemented in several countries to stop its local transmission. Considering this, the rapid screening and accurate detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2; 2019-nCoV) play an essential role in the diagnosis of COVID-19, which can minimize local transmission and prevent an epidemic. Due to this public health emergency, the development of ultra-fast reliable diagnostic kits is essential for the diagnosis of COVID-19. Recently, molecular biology and nanotechnology based analytical methods have proven to be promising diagnostic tools for the rapid screening of 2019-nCoV with high accuracy and precision. The main aim of this review is to provide a retrospective overview on the molecular biology tools (reverse transcription polymerase chain reaction (RT-PCR) and reverse transcription loop-mediated isothermal amplification (RT-LAMP)) and nanotechnology based analytical tools (enzyme-linked immunosorbent assay (ELISA), RT-PCR, and lateral flow assay) for the rapid diagnosis of COVID-19. This review also presents recent reports on other analytical techniques including paper spray mass spectrometry for the diagnosis of COVID-19 in clinical samples. Finally, we provide a quick reference on molecular biology and nanotechnology based analytical tools for COVID-19 diagnosis in clinical samples.
    MeSH term(s) Antibodies, Viral/blood ; COVID-19/diagnosis ; COVID-19/virology ; Humans ; Immunoassay ; Molecular Diagnostic Techniques ; Nanotechnology ; Nucleic Acid Amplification Techniques ; RNA, Viral/analysis ; Real-Time Polymerase Chain Reaction ; SARS-CoV-2/genetics ; SARS-CoV-2/isolation & purification ; SARS-CoV-2/metabolism ; Viral Proteins/genetics ; Viral Proteins/immunology
    Chemical Substances Antibodies, Viral ; RNA, Viral ; Viral Proteins
    Language English
    Publishing date 2021-02-05
    Publishing country England
    Document type Journal Article
    ZDB-ID 210747-8
    ISSN 1364-5528 ; 0003-2654
    ISSN (online) 1364-5528
    ISSN 0003-2654
    DOI 10.1039/d0an01528h
    Database MEDical Literature Analysis and Retrieval System OnLINE

    More links

    Kategorien

    In stock of ZB MED Cologne/Königswinter

    Zs.A 2423: Show issues Location:
    Je nach Verfügbarkeit (siehe Angabe bei Bestand)
    bis Jg. 1994: Bestellungen von Artikeln über das Online-Bestellformular
    Jg. 1995 - 2021: Lesesall (2.OG)
    ab Jg. 2022: Lesesaal (EG)

    Order via subito

    This service is chargeable due to the Delivery terms set by subito. Orders including an article and supplementary material will be classified as separate orders. In these cases, fees will be demanded for each order.

To top