Article ; Online: 3D-printed electrode as a new platform for electrochemical immunosensors for virus detection.
2020 Volume 1147, Page(s) 30–37
Abstract: Simple, low-cost, and sensitive new platforms for electrochemical immunosensors for virus detection ... immunosensor. The results showed the potentiality of the device for the quantitative detection of Hantavirus ... have been attracted attention due to the recent pandemic caused by a new type of coronavirus (SARS-CoV ...
| Abstract | Simple, low-cost, and sensitive new platforms for electrochemical immunosensors for virus detection have been attracted attention due to the recent pandemic caused by a new type of coronavirus (SARS-CoV-2). In the present work, we report for the first time the construction of an immunosensor using a commercial 3D conductive filament of carbon black and polylactic acid (PLA) to detect Hantavirus Araucaria nucleoprotein (Np) as a proof-of-concept. The recognition biomolecule was anchored directly at the filament surface by using N-(3-Dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride and N-Hydroxysuccinimide (EDC/NHS). Conductive and non-conductive composites of PLA were characterized using thermal gravimetric analysis (TGA), revealing around 30% w/w of carbon in the filament. Morphological features of composites were obtained from SEM and TEM measurements. FTIR measurement revealed that crosslinking agents were covalently bonded at the filament surface. Electrochemical techniques such as cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) were used for the evaluation of each step involved in the construction of the proposed immunosensor. The results showed the potentiality of the device for the quantitative detection of Hantavirus Araucaria nucleoprotein (Np) from 30 μg mL |
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| MeSH term(s) | Antibodies, Immobilized/chemistry ; Antibodies, Immobilized/immunology ; Antibodies, Viral/immunology ; COVID-19/diagnosis ; COVID-19/virology ; Dielectric Spectroscopy ; Electrodes ; Hantavirus/isolation & purification ; Hantavirus/metabolism ; Hantavirus Infections/diagnosis ; Hantavirus Infections/virology ; Humans ; Immunoassay/instrumentation ; Immunoassay/methods ; Limit of Detection ; Nucleocapsid Proteins/blood ; Nucleocapsid Proteins/immunology ; Printing, Three-Dimensional ; SARS-CoV-2/isolation & purification ; Soot/chemistry |
| Chemical Substances | Antibodies, Immobilized ; Antibodies, Viral ; Nucleocapsid Proteins ; Soot |
| Language | English |
| Publishing date | 2020-12-11 |
| Publishing country | Netherlands |
| Document type | Journal Article |
| ZDB-ID | 1483436-4 |
| ISSN | 1873-4324 ; 0003-2670 |
| ISSN (online) | 1873-4324 |
| ISSN | 0003-2670 |
| DOI | 10.1016/j.aca.2020.12.014 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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