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  1. Article ; Online: Identification of multiple potential viral diseases in a large urban center using wastewater surveillance.

    McCall, Camille / Wu, Huiyun / Miyani, Brijen / Xagoraraki, Irene

    Water research

    2020  Volume 184, Page(s) 116160

    Abstract: ... to identify enteric and non-enteric viruses collected from a large urban area for potential public health ... diseases reported in the catchment area. Metagenomics detected the presence of viral pathogens that cause clinically ... 2018 (n = 54) to evaluate the diversity of human viral pathogens in collected samples. Viruses were ...

    Abstract Viruses are linked to a multitude of human illnesses and can disseminate widely in urbanized environments causing global adverse impacts on communities and healthcare infrastructures. Wastewater-based epidemiology was employed using metagenomics and quantitative polymerase chain reaction (qPCR) assays to identify enteric and non-enteric viruses collected from a large urban area for potential public health monitoring and outbreak analysis. Untreated wastewater samples were collected from November 2017 to February 2018 (n = 54) to evaluate the diversity of human viral pathogens in collected samples. Viruses were classified into virus types based on primary transmission routes and reviewed against viral associated diseases reported in the catchment area. Metagenomics detected the presence of viral pathogens that cause clinically significant diseases reported within the study area during the sampling year. Detected viruses belong to the Adenoviridae, Astroviridae, Caliciviridae, Coronaviridae, Flaviviridae, Hepeviridae, Herpesviridae, Matonaviridae, Papillomaviridae, Parvoviridae, Picornaviridae, Poxviridae, Retroviridae, and Togaviridae families. Furthermore, concentrations of adenovirus, norovirus GII, sapovirus, hepatitis A virus, human herpesvirus 6, and human herpesvirus 8 were measured in wastewater samples and compared to metagenomic findings to confirm detected viral genus. Hepatitis A virus obtained the greatest average viral load (1.86 × 10
    MeSH term(s) Hepatitis A virus ; Humans ; Norovirus ; Sapovirus ; Viruses/genetics ; Waste Water
    Chemical Substances Waste Water
    Keywords covid19
    Language English
    Publishing date 2020-07-07
    Publishing country England
    Document type Journal Article
    ZDB-ID 202613-2
    ISSN 1879-2448 ; 0043-1354
    ISSN (online) 1879-2448
    ISSN 0043-1354
    DOI 10.1016/j.watres.2020.116160
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article: Identification of multiple potential viral diseases in a large urban center using wastewater surveillance

    McCall, Camille / Wu, Huiyun / Miyani, Brijen / Xagoraraki, Irene

    Water Res

    Abstract: ... to identify enteric and non-enteric viruses collected from a large urban area for potential public health ... This methodology has the potential to improve public health responses to large scale outbreaks and viral pandemics. ... 107 genome copies/L) in wastewater samples compared to other viruses quantified using qPCR with a 100 ...

    Abstract Viruses are linked to a multitude of human illnesses and can disseminate widely in urbanized environments causing global adverse impacts on communities and healthcare infrastructures. Wastewater-based epidemiology was employed using metagenomics and quantitative polymerase chain reaction (qPCR) assays to identify enteric and non-enteric viruses collected from a large urban area for potential public health monitoring and outbreak analysis. Untreated wastewater samples were collected from November 2017 to February 2018 (n = 54) to evaluate the diversity of human viral pathogens in collected samples. Viruses were classified into virus types based on primary transmission routes and reviewed against viral associated diseases reported in the catchment area. Metagenomics detected the presence of viral pathogens that cause clinically significant diseases reported within the study area during the sampling year. Detected viruses belong to the Adenoviridae, Astroviridae, Caliciviridae, Coronaviridae, Flaviviridae, Hepeviridae, Herpesviridae, Matonaviridae, Papillomaviridae, Parvoviridae, Picornaviridae, Poxviridae, Retroviridae, and Togaviridae families. Furthermore, concentrations of adenovirus, norovirus GII, sapovirus, hepatitis A virus, human herpesvirus 6, and human herpesvirus 8 were measured in wastewater samples and compared to metagenomic findings to confirm detected viral genus. Hepatitis A virus obtained the greatest average viral load (1.86 × 107 genome copies/L) in wastewater samples compared to other viruses quantified using qPCR with a 100% detection rate in metagenomic samples. Average concentration of sapovirus (1.36 × 106 genome copies/L) was significantly greater than norovirus GII (2.94 × 104 genome copies/L) indicating a higher burden within the study area. Findings obtained from this study aid in evaluating the utility of wastewater-based epidemiology for identification and routine monitoring of various viruses in large communities. This methodology has the potential to improve public health responses to large scale outbreaks and viral pandemics.
    Keywords covid19
    Publisher WHO
    Document type Article
    Note WHO #Covidence: #635427
    Database COVID19

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  3. Article: Identification of multiple potential viral diseases in a large urban center using wastewater surveillance

    McCall, Camille / Wu, Huiyun / Miyani, Brijen / Xagoraraki, Irene

    Water research. 2020 Oct. 01, v. 184

    2020  

    Abstract: ... to identify enteric and non-enteric viruses collected from a large urban area for potential public health ... This methodology has the potential to improve public health responses to large scale outbreaks and viral pandemics. ... 10⁷ genome copies/L) in wastewater samples compared to other viruses quantified using qPCR with a 100 ...

    Abstract Viruses are linked to a multitude of human illnesses and can disseminate widely in urbanized environments causing global adverse impacts on communities and healthcare infrastructures. Wastewater-based epidemiology was employed using metagenomics and quantitative polymerase chain reaction (qPCR) assays to identify enteric and non-enteric viruses collected from a large urban area for potential public health monitoring and outbreak analysis. Untreated wastewater samples were collected from November 2017 to February 2018 (n = 54) to evaluate the diversity of human viral pathogens in collected samples. Viruses were classified into virus types based on primary transmission routes and reviewed against viral associated diseases reported in the catchment area. Metagenomics detected the presence of viral pathogens that cause clinically significant diseases reported within the study area during the sampling year. Detected viruses belong to the Adenoviridae, Astroviridae, Caliciviridae, Coronaviridae, Flaviviridae, Hepeviridae, Herpesviridae, Matonaviridae, Papillomaviridae, Parvoviridae, Picornaviridae, Poxviridae, Retroviridae, and Togaviridae families. Furthermore, concentrations of adenovirus, norovirus GII, sapovirus, hepatitis A virus, human herpesvirus 6, and human herpesvirus 8 were measured in wastewater samples and compared to metagenomic findings to confirm detected viral genus. Hepatitis A virus obtained the greatest average viral load (1.86 × 10⁷ genome copies/L) in wastewater samples compared to other viruses quantified using qPCR with a 100% detection rate in metagenomic samples. Average concentration of sapovirus (1.36 × 10⁶ genome copies/L) was significantly greater than norovirus GII (2.94 × 10⁴ genome copies/L) indicating a higher burden within the study area. Findings obtained from this study aid in evaluating the utility of wastewater-based epidemiology for identification and routine monitoring of various viruses in large communities. This methodology has the potential to improve public health responses to large scale outbreaks and viral pandemics.
    Keywords Adenoviridae ; Astroviridae ; Coronaviridae ; Flaviviridae ; Hepatovirus A ; Hepeviridae ; Human betaherpesvirus 6 ; Human gammaherpesvirus 8 ; Papillomaviridae ; Parvoviridae ; Poxviridae ; Retroviridae ; Sapovirus ; Togaviridae ; genome ; health services ; humans ; metagenomics ; monitoring ; primary transmission ; public health ; quantitative polymerase chain reaction ; research ; urban areas ; urbanization ; viral load ; viruses ; wastewater ; water ; watersheds
    Language English
    Dates of publication 2020-1001
    Publishing place Elsevier Ltd
    Document type Article
    Note NAL-AP-2-clean
    ZDB-ID 202613-2
    ISSN 1879-2448 ; 0043-1354
    ISSN (online) 1879-2448
    ISSN 0043-1354
    DOI 10.1016/j.watres.2020.116160
    Database NAL-Catalogue (AGRICOLA)

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