Article ; Online: Biophysical characterization of Vpu from HIV-1 suggests a channel-pore dualism.
2007 Volume 70, Issue 4, Page(s) 1488–1497
Abstract: ... peptide, recordings for Vpu(1-32)-S24L do not exhibit any noticeable channel activity. Recordings of WT ... Vpu from HIV-1 is an 81 amino acid type I integral membrane protein which consists of a cytoplasmic ... Both peptide channels follow the Eisenman series I, indicative for a weak ion channel with almost pore like ...
| Abstract | Vpu from HIV-1 is an 81 amino acid type I integral membrane protein which consists of a cytoplasmic and a transmembrane (TM) domain. The TM domain is known to alter membrane permeability for ions and substrates when inserted into artificial membranes. Peptides corresponding to the TM domain of Vpu (Vpu(1-32)) and mutant peptides (Vpu(1-32)-W23L, Vpu(1-32)-R31V, Vpu(1-32)-S24L) have been synthesized and reconstituted into artificial lipid bilayers. All peptides show channel activity with a main conductance level of around 20 pS. Vpu(1-32)-W23L has a considerable flickering pattern in the recordings and longer open times than Vpu(1-32). Whilst recordings for Vpu(1-32)-R31V are almost indistinguishable from those of the WT peptide, recordings for Vpu(1-32)-S24L do not exhibit any noticeable channel activity. Recordings of WT peptide and Vpu(1-32)-W23L indicate Michaelis-Menten behavior when the salt concentration is increased. Both peptide channels follow the Eisenman series I, indicative for a weak ion channel with almost pore like characteristics. |
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| MeSH term(s) | Human Immunodeficiency Virus Proteins/chemistry ; Human Immunodeficiency Virus Proteins/genetics ; Human Immunodeficiency Virus Proteins/physiology ; Humans ; Ion Channels ; Kinetics ; Lipid Bilayers/metabolism ; Osmolar Concentration ; Peptide Fragments/chemistry ; Peptide Fragments/genetics ; Viral Regulatory and Accessory Proteins/chemistry ; Viral Regulatory and Accessory Proteins/genetics ; Viral Regulatory and Accessory Proteins/physiology | |||||
| Chemical Substances | Human Immunodeficiency Virus Proteins ; Ion Channels ; Lipid Bilayers ; Peptide Fragments ; Viral Regulatory and Accessory Proteins ; vpu protein, Human immunodeficiency virus 1 | |||||
| Keywords | covid19 | |||||
| Language | English | |||||
| Publishing date | 2007-10-02 | |||||
| Publishing country | United States | |||||
| Document type | Journal Article ; Research Support, Non-U.S. Gov't | |||||
| ZDB-ID | 806683-8 | |||||
| ISSN | 1097-0134 ; 0887-3585 | |||||
| ISSN (online) | 1097-0134 | |||||
| ISSN | 0887-3585 | |||||
| DOI | 10.1002/prot.21642 | |||||
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| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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