Article ; Online: Two Detailed Plaque Assay Protocols for the Quantification of Infectious SARS-CoV-2.
Current protocols in microbiology
2020 Volume 57, Issue 1, Page(s) ecpmc105
Abstract: ... to quantitate infectious virions. Measurement of infectious SARS-CoV-2 can be achieved by tissue culture ... Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has been identified as the causal ... 2019. While SARS-CoV-2 titers can be measured by detection of viral nucleic acid, this method is unable ...
| Abstract | Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has been identified as the causal agent of COronaVIrus Disease-19 (COVID-19), an atypical pneumonia-like syndrome that emerged in December 2019. While SARS-CoV-2 titers can be measured by detection of viral nucleic acid, this method is unable to quantitate infectious virions. Measurement of infectious SARS-CoV-2 can be achieved by tissue culture infectious dose-50 (TCID |
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| MeSH term(s) | Animals ; Betacoronavirus/isolation & purification ; Chlorocebus aethiops ; Clinical Protocols ; Humans ; SARS-CoV-2 ; Staining and Labeling ; Vero Cells ; Viral Plaque Assay/methods |
| Keywords | covid19 |
| Language | English |
| Publishing date | 2020-06-19 |
| Publishing country | United States |
| Document type | Journal Article ; Research Support, Non-U.S. Gov't |
| ISSN | 1934-8533 |
| ISSN (online) | 1934-8533 |
| DOI | 10.1002/cpmc.105 |
| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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