Article: Upregulated expression of Iba1 molecules in the central nervous system of mice in response to neurovirulent influenza A virus infection.
2000 Volume 44, Issue 8, Page(s) 729–735
Abstract: ... upregulated in the mouse brain in response to influenza virus infection and may play significant roles ... in the brain after stereotaxic introduction of the neurovirulent WSN strain of influenza A virus ... into the olfactory bulb of C57BL/6 mice. The virus selectively targeted the paraventricular and anterior olfactory nuclei ...
| Abstract | The present study deals with the expression of Iba1 molecules, a novel EF-hand Ca2+-binding protein, in the brain after stereotaxic introduction of the neurovirulent WSN strain of influenza A virus into the olfactory bulb of C57BL/6 mice. The virus selectively targeted the paraventricular and anterior olfactory nuclei. Infected neurons appeared as early as at day 3 post infection and degenerated and vanished by day 12. The Iba1 molecule was normally expressed in resting microglia. The overexpression of the Iba1 in microglial cells was detected at day 3 post infection, culminating at day 7 with a morphological activation. Iba1-immunopositive macrophages outnumbered microglia in the paraventricular and anterior olfactory nuclei, where the infected neurons had degenerated. Macrophages totally disappeared by day 12, and the Iba1-expression in microglia was reduced to a normal level by day 35. Lack of perforin predisposed the mice to long-term virus infection of the brain, leading to the prolonged Iba1-overexpression. These results show that the Iba1 is upregulated in the mouse brain in response to influenza virus infection and may play significant roles in the regulation of some immunological and pathophysiological functions of microglia during virus infection. |
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| MeSH term(s) | Animals ; Antigens, Viral/analysis ; Brain/metabolism ; Brain/virology ; Calcium-Binding Proteins/metabolism ; Female ; Gene Expression Regulation ; Influenza A virus/immunology ; Macrophages/metabolism ; Membrane Glycoproteins/genetics ; Mice ; Mice, Inbred C57BL ; Mice, Knockout ; Microfilament Proteins ; Microglia/metabolism ; Orthomyxoviridae Infections/metabolism ; Orthomyxoviridae Infections/virology ; Perforin ; Pore Forming Cytotoxic Proteins | |||||
| Chemical Substances | Aif1 protein, mouse ; Antigens, Viral ; Calcium-Binding Proteins ; Membrane Glycoproteins ; Microfilament Proteins ; Pore Forming Cytotoxic Proteins ; Perforin (126465-35-8) | |||||
| Language | English | |||||
| Publishing date | 2000 | |||||
| Publishing country | Australia | |||||
| Document type | Journal Article ; Research Support, Non-U.S. Gov't | |||||
| ZDB-ID | 224792-6 | |||||
| ISSN | 1348-0421 ; 0385-5600 | |||||
| ISSN (online) | 1348-0421 | |||||
| ISSN | 0385-5600 | |||||
| DOI | 10.1111/j.1348-0421.2000.tb02556.x | |||||
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| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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