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  1. Article ; Online: SARS-CoV-2 elicits robust adaptive immune responses regardless of disease severity.

    Nielsen, Stine Sf / Vibholm, Line K / Monrad, Ida / Olesen, Rikke / Frattari, Giacomo S / Pahus, Marie H / Højen, Jesper F / Gunst, Jesper D / Erikstrup, Christian / Holleufer, Andreas / Hartmann, Rune / Østergaard, Lars / Søgaard, Ole S / Schleimann, Mariane H / Tolstrup, Martin

    EBioMedicine

    2021  Volume 68, Page(s) 103410

    Abstract: ... vaccines, comprehensive characterization of adaptive immune responses towards SARS-CoV-2 is needed ... Background: The SARS-CoV-2 pandemic currently prevails worldwide. To understand the immunological ... signature of SARS-CoV-2 infections and aid the search and evaluation of new treatment modalities and ...

    Abstract Background: The SARS-CoV-2 pandemic currently prevails worldwide. To understand the immunological signature of SARS-CoV-2 infections and aid the search and evaluation of new treatment modalities and vaccines, comprehensive characterization of adaptive immune responses towards SARS-CoV-2 is needed.
    Methods: We included 203 recovered SARS-CoV-2 infected patients in Denmark between April 3
    Findings: We report broad serological profiles within the cohort, detecting antibody binding to other human coronaviruses. 202(>99%) participants had SARS-CoV-2 specific antibodies, with SARS-CoV-2 neutralization and spike-ACE2 receptor interaction blocking observed in 193(95%) individuals. A significant positive correlation (r=0.7804) between spike-ACE2 blocking antibody titers and neutralization potency was observed. Further, SARS-CoV-2 specific CD8
    Interpretation: The viral surface spike protein was identified as the dominant target for both neutralizing antibodies and CD8
    Funding: This study was supported by the Danish Ministry for Research and Education (grant# 0238-00001B) and The Danish Innovation Fund (grant# 0208-00018B).
    MeSH term(s) Adaptive Immunity ; Adult ; Aged ; Angiotensin-Converting Enzyme 2/metabolism ; Animals ; Antibodies, Neutralizing/blood ; Antibodies, Viral/blood ; CD8-Positive T-Lymphocytes/metabolism ; COVID-19/immunology ; COVID-19/virology ; Cell Line ; Denmark ; Female ; Humans ; Male ; Middle Aged ; SARS-CoV-2/immunology ; SARS-CoV-2/pathogenicity ; Severity of Illness Index ; Spike Glycoprotein, Coronavirus/immunology ; Young Adult
    Chemical Substances Antibodies, Neutralizing ; Antibodies, Viral ; Spike Glycoprotein, Coronavirus ; spike protein, SARS-CoV-2 ; ACE2 protein, human (EC 3.4.17.23) ; Angiotensin-Converting Enzyme 2 (EC 3.4.17.23)
    Language English
    Publishing date 2021-06-04
    Publishing country Netherlands
    Document type Journal Article
    ZDB-ID 2851331-9
    ISSN 2352-3964
    ISSN (online) 2352-3964
    DOI 10.1016/j.ebiom.2021.103410
    Database MEDical Literature Analysis and Retrieval System OnLINE

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  2. Article ; Online: SARS-CoV-2 elicits robust adaptive immune responses regardless of disease severity

    Stine SF Nielsen / Line K Vibholm / Ida Monrad / Rikke Olesen / Giacomo S Frattari / Marie H Pahus / Jesper F Højen / Jesper D Gunst / Christian Erikstrup / Andreas Holleufer / Rune Hartmann / Lars Østergaard / Ole S Søgaard / Mariane H Schleimann / Martin Tolstrup

    EBioMedicine, Vol 68, Iss , Pp 103410- (2021)

    2021  

    Abstract: ... vaccines, comprehensive characterization of adaptive immune responses towards SARS-CoV-2 is needed. Methods ... for both neutralizing antibodies and CD8+ T-cell responses. Overall, the majority of patients had robust adaptive immune responses ... compared amongst severity groups within the cohort, and 10 pre-pandemic SARS-CoV-2 negative controls ...

    Abstract Background: The SARS-CoV-2 pandemic currently prevails worldwide. To understand the immunological signature of SARS-CoV-2 infections and aid the search and evaluation of new treatment modalities and vaccines, comprehensive characterization of adaptive immune responses towards SARS-CoV-2 is needed. Methods: We included 203 recovered SARS-CoV-2 infected patients in Denmark between April 3rd and July 9th 2020, at least 14 days after COVID-19 symptom recovery. The participants had experienced a range of disease severities from asymptomatic to severe. We collected plasma, serum and PBMC's for analysis of SARS-CoV-2 specific antibody response by Meso Scale analysis including other coronavirus strains, ACE2 competition, IgA ELISA, pseudovirus neutralization capacity, and dextramer flow cytometry analysis of CD8+ T cells. The immunological outcomes were compared amongst severity groups within the cohort, and 10 pre-pandemic SARS-CoV-2 negative controls. Findings: We report broad serological profiles within the cohort, detecting antibody binding to other human coronaviruses. 202(>99%) participants had SARS-CoV-2 specific antibodies, with SARS-CoV-2 neutralization and spike-ACE2 receptor interaction blocking observed in 193(95%) individuals. A significant positive correlation (r=0.7804) between spike-ACE2 blocking antibody titers and neutralization potency was observed. Further, SARS-CoV-2 specific CD8+ T-cell responses were clear and quantifiable in 95 of 106(90%) HLA-A2+ individuals. Interpretation: The viral surface spike protein was identified as the dominant target for both neutralizing antibodies and CD8+ T-cell responses. Overall, the majority of patients had robust adaptive immune responses, regardless of their disease severity. Funding: This study was supported by the Danish Ministry for Research and Education (grant# 0238-00001B) and The Danish Innovation Fund (grant# 0208-00018B)
    Keywords SARS-CoV-2 ; COVID-19 ; Antibody ; CD8+ T-cell ; Immune response ; Adaptive ; Medicine ; R ; Medicine (General) ; R5-920
    Subject code 616
    Language English
    Publishing date 2021-06-01T00:00:00Z
    Publisher Elsevier
    Document type Article ; Online
    Database BASE - Bielefeld Academic Search Engine (life sciences selection)

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  3. Article ; Online: SARS-CoV-2 Elicits Robust Adaptive Immune Responses Regardless of Disease Severity

    Nielsen, Stine Sofie Frank / Vibholm, Line K / Monrad, Ida / Olesen, Rikke / Frattari, Giacomo S / Pahus, Marie H / Højen, Jesper F / Gunst, Jesper D / Erikstrup, Christian / Holleufer, Andreas / Hartmann, Rune / Østergaard, Lars / Søgaard, Ole S / Schleimann, Mariane H / Tolstrup, Martin

    bioRxiv

    Abstract: ... Overall, the majority of patients had robust adaptive immune responses, regardless of disease severity ... of adaptive immune responses towards SARS-CoV-2 is needed. We investigated the breadth and potency of antibody-, and T ... cell immune responses, in 203 recovered SARS-CoV-2 infected patients who presented with asymptomatic to severe ...

    Abstract The SARS-CoV-2 pandemic currently prevails worldwide. To understand the immunological signature of SARS-CoV-2 infections and aid the search for treatments and vaccines, comprehensive characterization of adaptive immune responses towards SARS-CoV-2 is needed. We investigated the breadth and potency of antibody-, and T-cell immune responses, in 203 recovered SARS-CoV-2 infected patients who presented with asymptomatic to severe infections. We report very broad serological profiles with cross-reactivity to other human coronaviruses. Further, >99% had SARS-CoV-2 epitope specific antibodies, with SARS-CoV-2 neutralization and spike-ACE2 receptor interaction blocking observed in 95% of individuals. A significant positive correlation between spike-ACE2 blocking antibody titers and neutralization potency was observed. SARS-CoV-2 specific CD8+ T-cell responses were clear and quantifiable in 90% of HLA-A2+ individuals. The viral surface spike protein was identified as the dominant target for both neutralizing antibodies and CD8+ T cell responses. Overall, the majority of patients had robust adaptive immune responses, regardless of disease severity. These data support the possibility of achieving protective immunity through natural infection and bode well for the prospects of inducing immunological memory through vaccination.
    Keywords covid19
    Publisher BioRxiv; WHO
    Document type Article ; Online
    Note WHO #Covidence: #331645
    DOI 10.1101/2020.10.08.331645
    Database COVID19

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  4. Article ; Online: SARS-CoV-2 Elicits Robust Adaptive Immune Responses Regardless of Disease Severity

    Nielsen, Stine Sofie Frank / Vibholm, Line K / Monrad, Ida / Olesen, Rikke / Frattari, Giacomo S / Pahus, Marie H / Højen, Jesper F / Gunst, Jesper D / Erikstrup, Christian / Holleufer, Andreas / Hartmann, Rune / Østergaard, Lars / Søgaard, Ole S / Schleimann, Mariane H / Tolstrup, Martin

    bioRxiv

    Abstract: ... Overall, the majority of patients had robust adaptive immune responses, regardless of disease severity ... of adaptive immune responses towards SARS-CoV-2 is needed. We investigated the breadth and potency of antibody-, and T ... cell immune responses, in 203 recovered SARS-CoV-2 infected patients who presented with asymptomatic to severe ...

    Abstract The SARS-CoV-2 pandemic currently prevails worldwide. To understand the immunological signature of SARS-CoV-2 infections and aid the search for treatments and vaccines, comprehensive characterization of adaptive immune responses towards SARS-CoV-2 is needed. We investigated the breadth and potency of antibody-, and T-cell immune responses, in 203 recovered SARS-CoV-2 infected patients who presented with asymptomatic to severe infections. We report very broad serological profiles with cross-reactivity to other human coronaviruses. Further, >99% had SARS-CoV-2 epitope specific antibodies, with SARS-CoV-2 neutralization and spike-ACE2 receptor interaction blocking observed in 95% of individuals. A significant positive correlation between spike-ACE2 blocking antibody titers and neutralization potency was observed. SARS-CoV-2 specific CD8+ T-cell responses were clear and quantifiable in 90% of HLA-A2+ individuals. The viral surface spike protein was identified as the dominant target for both neutralizing antibodies and CD8+ T cell responses. Overall, the majority of patients had robust adaptive immune responses, regardless of disease severity. These data support the possibility of achieving protective immunity through natural infection and bode well for the prospects of inducing immunological memory through vaccination.
    Keywords covid19
    Language English
    Publishing date 2020-10-09
    Publisher Cold Spring Harbor Laboratory
    Document type Article ; Online
    DOI 10.1101/2020.10.08.331645
    Database COVID19

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