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  1. Article ; Online: Neuroanatomical organization of methionine-enkephalinergic system in the brain of the Mozambique tilapia Oreochromis mossambicus.

    Ganesh, C B / Vijayalaxmi

    Journal of chemical neuroanatomy

    2021  Volume 115, Page(s) 101963

    Abstract: ... gustatory nucleus, the sensory trigeminal nerve nucleus, the nucleus reticularis medialis and the vagal ... were located in magnocellular and parvocellular subdivisions of the nucleus preopticus, and medial and ... lateral subdivisions of the nucleus lateralis tuberis. Surrounding the recessus lateralis ...

    Abstract Enkephalins are a class of opioid peptides implicated in several physiological and neuroendocrine responses in vertebrates. In this study, using immunocytochemical or immunofluorescence technique, we examined the neuroanatomical distribution of methionine enkephalin (M-ENK) immunoreactivity in the central nervous system (CNS) of the cichlid fish Oreochromis mossambicus. In the telencephalon, no M-ENK-like-immunoreactive (M-ENK-L-ir) perikarya, but sparsely distributed fibres were detected in the glomerular layer and the granule cell layer of the olfactory bulb. Although intensely labeled M-ENK-L-ir cells and fibres were found in the pallium, no M-ENK immunoreactivity was observed in the subpallium. The preoptic area showed a few M-ENK-L-ir somata and dense innervations of fibres. In the hypothalamic area, M-ENK-L-ir cells and fibres were located in magnocellular and parvocellular subdivisions of the nucleus preopticus, and medial and lateral subdivisions of the nucleus lateralis tuberis. Surrounding the recessus lateralis of the third ventricle, several intensely stained and packed M-ENK-L-ir cells and fibres were seen in dorsal, lateral and ventral subdivisions of the nucleus recessus lateralis. In the diencephalon, M-ENK immunoreactivity was restricted to the habenula, the thalamus, the pretectal area and the nucleus posterior tuberis. Dense aggregations of M-ENK-L-ir fibres were seen in the mesencephalic subdivisions, the optic tectum and the torus semicircularis, whereas a few fusiform M-ENK-L-ir cells and fibres were scattered in the midbrain tegmentum. In the rhombencephalon, different populations of ovoid or spindle shaped M-ENK-L-ir cells were observed in the secondary gustatory nucleus, the sensory trigeminal nerve nucleus, the nucleus reticularis medialis and the vagal motor nucleus, whereas bands of fibres were seen in the rostral spinal cord. Collectively, the widespread distribution of M-ENK immunoreactivity in the CNS suggests a role for this opioid peptide in regulation of neuroendocrine control of reproduction and modulation of sensorimotor functions in fish.
    MeSH term(s) Animals ; Brain/metabolism ; Enkephalin, Methionine/metabolism ; Neurons/metabolism ; Tilapia/metabolism
    Chemical Substances Enkephalin, Methionine (58569-55-4)
    Language English
    Publishing date 2021-05-03
    Publishing country Netherlands
    Document type Journal Article ; Research Support, Non-U.S. Gov't
    ZDB-ID 639443-7
    ISSN 1873-6300 ; 0891-0618
    ISSN (online) 1873-6300
    ISSN 0891-0618
    DOI 10.1016/j.jchemneu.2021.101963
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  2. Article ; Online: Cholecystokinin in the central nervous system of the sea lamprey Petromyzon marinus: precursor identification and neuroanatomical relationships with other neuronal signalling systems.

    Sobrido-Cameán, D / Yáñez-Guerra, L A / Robledo, D / López-Varela, E / Rodicio, M C / Elphick, M R / Anadón, R / Barreiro-Iglesias, Antón

    Brain structure & function

    2019  Volume 225, Issue 1, Page(s) 249–284

    Abstract: ... This revealed PmCCK-expressing neurons in the hypothalamus, posterior tubercle, prethalamus, nucleus ... the putative nucleus of the solitary tract. Some PmCCK-expressing neuronal populations were only observed ... reminiscent of the secondary gustatory/visceral tract of teleosts. In conclusion, this study provides ...

    Abstract Cholecystokinin (CCK) is a neuropeptide that modulates processes such as digestion, satiety, and anxiety. CCK-type peptides have been characterized in jawed vertebrates and invertebrates, but little is known about CCK-type signalling in the most ancient group of vertebrates, the agnathans. Here, we have cloned and sequenced a cDNA encoding a sea lamprey (Petromyzon marinus L.) CCK-type precursor (PmCCK), which contains a CCK-type octapeptide sequence (PmCCK-8) that is highly similar to gnathostome CCKs. Using mRNA in situ hybridization, the distribution of PmCCK-expressing neurons was mapped in the CNS of P. marinus. This revealed PmCCK-expressing neurons in the hypothalamus, posterior tubercle, prethalamus, nucleus of the medial longitudinal fasciculus, midbrain tegmentum, isthmus, rhombencephalic reticular formation, and the putative nucleus of the solitary tract. Some PmCCK-expressing neuronal populations were only observed in adults, revealing important differences with larvae. We generated an antiserum to PmCCK-8 to enable immunohistochemical analysis of CCK expression, which revealed that GABA or glutamate, but not serotonin, tyrosine hydroxylase or neuropeptide Y, is co-expressed in some PmCCK-8-immunoreactive (ir) neurons. Importantly, this is the first demonstration of co-localization of GABA and CCK in neurons of a non-mammalian vertebrate. We also characterized extensive cholecystokinergic fibre systems of the CNS, including innervation of habenular subnuclei. A conspicuous PmCCK-8-ir tract ascending in the lateral rhombencephalon selectively innervates a glutamatergic population in the dorsal isthmic grey. Interestingly, this tract is reminiscent of the secondary gustatory/visceral tract of teleosts. In conclusion, this study provides important new information on the evolution of the cholecystokinergic system in vertebrates.
    MeSH term(s) Animals ; Biological Evolution ; Brain/cytology ; Brain/metabolism ; Cholecystokinin/metabolism ; DNA, Complementary/metabolism ; In Situ Hybridization ; Neurons/cytology ; Neurons/metabolism ; Petromyzon/anatomy & histology ; Petromyzon/metabolism ; Protein Precursors/metabolism ; RNA, Messenger/metabolism ; Sexual Maturation ; Signal Transduction ; gamma-Aminobutyric Acid/metabolism
    Chemical Substances DNA, Complementary ; Protein Precursors ; RNA, Messenger ; gamma-Aminobutyric Acid (56-12-2) ; procholecystokinin (78206-77-6) ; Cholecystokinin (9011-97-6)
    Language English
    Publishing date 2019-12-05
    Publishing country Germany
    Document type Journal Article
    ZDB-ID 2273162-3
    ISSN 1863-2661 ; 1863-2653
    ISSN (online) 1863-2661
    ISSN 1863-2653
    DOI 10.1007/s00429-019-01999-2
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  3. Article: Neuroanatomical substrates involved in the control of food intake.

    Kuenzel, W J

    Poultry science

    1989  Volume 68, Issue 7, Page(s) 926–937

    Abstract: ... The fifth pathway involves an interaction of the hypothalamus and the dorsal motor nucleus of the vagus ... pathway, the gustatory system, the olfactory pathway, and the autonomic nervous system/parasympathetic ... that the gustatory system is better developed than once thought. The fourth pathway involves the olfactory system ...

    Abstract Five neural pathways were reviewed regarding their specific role in the control of food intake in birds. The five pathways included the trigeminal sensorimotor system, the visual system/basal ganglia pathway, the gustatory system, the olfactory pathway, and the autonomic nervous system/parasympathetic pathway. The trigeminal system is the pathway best understood among the five systems associated with feeding. It begins with sensory nerves innervating the upper and lower mandibles and buccal cavity and ends with nerves projecting to jaw muscles. The function of the pathway is to control the grasping and mandibulation of pellets or seeds. The visual system includes both the tectofugal and thalamofugal pathways. Both visual pathways interact with the avian paleostriatal complex. The latter is equivalent to the mammalian basal ganglia. The second pathway is important in food recognition as well as in orienting the body with respect to its position in three-dimensional space. The third neural circuit involves the sense of taste. Approximately 300 taste buds have been identified within the buccal cavity of the chicken, suggesting that the gustatory system is better developed than once thought. The fourth pathway involves the olfactory system; as in the visual system, more than one pathway has been identified. The dominant pathway appears to project to the piriform cortex, a structure that may play a role in monitoring essential amino acid contents of the brain. The fifth pathway involves an interaction of the hypothalamus and the dorsal motor nucleus of the vagus. This pathway is important in activating the parasympathetic nervous system and in preparing an organism to feed. All five pathways play different roles in controlling food intake in birds.
    MeSH term(s) Animals ; Birds/physiology ; Eating ; Neural Pathways/physiology
    Language English
    Publishing date 1989-07
    Publishing country England
    Document type Journal Article ; Research Support, Non-U.S. Gov't ; Review
    ZDB-ID 242586-5
    ISSN 1525-3171 ; 0032-5791
    ISSN (online) 1525-3171
    ISSN 0032-5791
    DOI 10.3382/ps.0680926
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  4. Article: Neuroanatomical alterations in the rat nucleus of the solitary tract following early maternal NaCl deprivation and subsequent NaCl repletion.

    King, C T / Hill, D L

    The Journal of comparative neurology

    1993  Volume 333, Issue 4, Page(s) 531–542

    Abstract: ... were studied within the area of the nucleus of the solitary tract (NTS). Examination of dendritic ... central neurons, and glia may direct development within the central gustatory system. ...

    Abstract Restricting the NaCl content in the rat maternal and preweaning diet results in a significant and specific reduction (60%) of chorda tympani nerve responsiveness to sodium stimuli in the offspring. Repletion of dietary sodium at any time during postnatal development results in a complete and persistent recovery of chorda tympani nerve function. To learn whether the maturation of postsynaptic cells are also affected by the early dietary manipulation, dendritic morphology, neuronal and glial densities and numbers were studied within the area of the nucleus of the solitary tract (NTS). Examination of dendritic morphologies in Golgi-Cox stained neurons revealed that cells with multipolar and fusiform somata in the rostral NTS exhibited longer dendrites following dietary NaCl deprivation during development (deprived rats) than in controls. These changes were generally maintained in rats initially deprived of NaCl and then fed a NaCl-replete diet postweaning ("recovered" rats). In contrast, ovoid neurons were not affected by NaCl deprivation but had increases in the lengths of their dendrites following "recovery." Along with dendritic alterations, the packing density of neurons in the rostral NTS was greater in NaCl-deprived rats than in controls, but was similar to controls following "recovery." Glial packing density also increased following deprivation and remained high in "recovered" rats. These results indicate that activity-dependent events as well as events not related to afferent activity (e.g., hormonal changes) may influence the morphological development of NTS neurons. In addition, significant interactions among primary afferent fibers, central neurons, and glia may direct development within the central gustatory system.
    MeSH term(s) Animals ; Diet, Sodium-Restricted/adverse effects ; Female ; Histocytochemistry ; Medulla Oblongata/anatomy & histology ; Medulla Oblongata/cytology ; Medulla Oblongata/drug effects ; Neuroglia/drug effects ; Neuroglia/ultrastructure ; Neurons/drug effects ; Neurons/ultrastructure ; Pregnancy ; Rats ; Sodium, Dietary/pharmacology ; Taste/drug effects ; Taste/physiology
    Chemical Substances Sodium, Dietary
    Language English
    Publishing date 1993-07-22
    Publishing country United States
    Document type Journal Article ; Research Support, U.S. Gov't, P.H.S.
    ZDB-ID 3086-7
    ISSN 1096-9861 ; 0021-9967 ; 0092-7317
    ISSN (online) 1096-9861
    ISSN 0021-9967 ; 0092-7317
    DOI 10.1002/cne.903330406
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  5. Article: Electrophysiological and neuroanatomical studies of hepatic portal osmo- and sodium-receptive afferent projections within the brain.

    Rogers, R C / Novin, D / Butcher, L L

    Journal of the autonomic nervous system

    1979  Volume 1, Issue 2, Page(s) 183–202

    Abstract: ... from the nucleus of the solitary tract to the paraventricular and supraoptic nuclei it seems likely that the viscero-gustatory path ... verified that a path between the nucleus of the solitary tract, the parabrachial nucleus and ... from the ventrobasal complex to the supraoptic nuclei, from the parabrachial nucleus to the supraoptic nuclei and ...

    Abstract Previous investigations concerning the function of hepatic sodium and osmoreceptors indicated that the activation of these chemoreceptive structures significantly affected physiological and behavioral mechanisms related to water and electrolyte homeostasis. Though anatomical studies predicted that such information should follow the previously described gustatory afferent pathy, until recently, the precise course taken by higher order hepatic afferents was unknown. The studies described herein verify anatomical predictions with regard to the central course of vagal-visceral afferents, as hepatically activated neurons were localized to two areas known to relay gustatory input. Further, horseradish peroxidase histochemical studies verified that a path between the nucleus of the solitary tract, the parabrachial nucleus and the ventrobasal complex certainly exists. In the light of recent findings regarding projections from the ventrobasal complex to the supraoptic nuclei, from the parabrachial nucleus to the supraoptic nuclei and from the nucleus of the solitary tract to the paraventricular and supraoptic nuclei it seems likely that the viscero-gustatory path which carries hepatic afferent information is the pathway responsible for the physiological and perhaps the behavioral consequences of hepatic sodium or osmotic stimulation.
    MeSH term(s) Afferent Pathways/physiology ; Animals ; Brain Mapping ; Liver/innervation ; Male ; Pons/anatomy & histology ; Pons/physiology ; Rats ; Sensory Receptor Cells/physiology ; Sodium/physiology ; Thalamus/anatomy & histology ; Thalamus/physiology ; Vagotomy ; Vagus Nerve/anatomy & histology ; Vagus Nerve/physiology ; Water-Electrolyte Balance
    Chemical Substances Sodium (9NEZ333N27)
    Language English
    Publishing date 1979-12
    Publishing country Netherlands
    Document type Journal Article ; Research Support, U.S. Gov't, P.H.S.
    ZDB-ID 446196-4
    ISSN 1872-7476 ; 0165-1838
    ISSN (online) 1872-7476
    ISSN 0165-1838
    DOI 10.1016/0165-1838(79)90016-x
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  6. Article: Le noyau du faisceau solitaire: aspects neuroanatomiques, neurochimiques et fonctionnels.

    Jean, A

    Archives internationales de physiologie, de biochimie et de biophysique

    1991  Volume 99, Issue 5, Page(s) A3–52

    Abstract: ... the organization of both the cells and the fibrillar network are not homogeneous within the nucleus and the NTS has ... of short connections to bulbo-ponto-mesencephalic structures (parabrachial nucleus, motor nuclei ... of the structures that receive a direct projection from the NTS project back to the nucleus. Direct projections ...

    Title translation The nucleus tractus solitarius: neuroanatomic, neurochemical and functional aspects.
    Abstract The nucleus tractus solitarii (NTS) has long been considered as the first central relay for gustatory and visceral afferent informations only. However, data obtained during the past ten years, with neuroanatomical, biochemical and electrophysiological techniques, clearly demonstrate that the NTS is a structure with a high degree of complexity, which plays, at the medullary level, a key role in several integrative processes. The NTS, located in the dorsomedial medulla, is a structure of small size containing a limited number of neurons scattered in a more or less dense fibrillar plexus. The distribution and the organization of both the cells and the fibrillar network are not homogeneous within the nucleus and the NTS has been divided cytoarchitectonically into various subnuclei, which are partly correlated with the areas of projection of peripheral afferent endings. At the ultrastructural level, the NTS shows several complex synaptic arrangements in form of glomeruli. These arrangements provide morphological substrates for complex mechanisms of intercellular communication within the NTS. The NTS is not only the site of vagal and glossopharyngeal afferent projections, it receives also endings from facial and trigeminal nerves as well as from some renal afferents. Gustatory and somatic afferents from the oropharyngeal region project with a crude somatotopy within the rostral part of the NTS and visceral afferents from cardiovascular, digestive, respiratory and renal systems terminate viscero-topically within its caudal part. Moreover the NTS is extensively connected with several central structures. It projects directly to multiple brain regions by means of short connections to bulbo-ponto-mesencephalic structures (parabrachial nucleus, motor nuclei of several cranial nerves, ventro-lateral reticular formation, raphe nuclei...) and long connections to the spinal cord and diencephalic and telencephalic structures, in particular the hypothalamus and some limbic structures. The NTS is also the recipient of several central afferent inputs. It is worth to note that most of the structures that receive a direct projection from the NTS project back to the nucleus. Direct projections from the cerebral cortex to the NTS have also been identified. These extensive connections indicate that the NTS is a key structure for autonomic and neuroendocrine functions as well as for integration of somatic and autonomic responses in certain behaviors. The NTS contains a great diversity of neuroactive substances. Indeed, most of the substances identified within the central nervous system have also been detected in the NTS and may act, at this level, as classical transmitters and/or neuromodulators.(ABSTRACT TRUNCATED AT 400 WORDS)
    MeSH term(s) Amino Acids/physiology ; Animals ; Biogenic Amines/metabolism ; Medulla Oblongata/anatomy & histology ; Medulla Oblongata/chemistry ; Medulla Oblongata/physiology ; Neuropeptides/physiology
    Chemical Substances Amino Acids ; Biogenic Amines ; Neuropeptides
    Language French
    Publishing date 1991-09
    Publishing country Belgium
    Document type English Abstract ; Journal Article ; Review
    ZDB-ID 1076-5
    ISSN 0778-3124 ; 0003-9799
    ISSN 0778-3124 ; 0003-9799
    DOI 10.3109/13813459109145916
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