Article ; Online: β-Pix directs collective migration of anterior visceral endoderm cells in the early mouse embryo.
2014 Volume 28, Issue 24, Page(s) 2764–2777
Abstract: ... migration of anterior visceral endoderm (AVE) cells in the early mouse embryo. To identify potential ... localization of Rac1 activity to drive collective AVE migration at a critical stage in mouse development. ... guanine nucleotide exchange factor [GEF]) in an in vitro collective epithelial migration assay and identified β-Pix. Genetic deletion ...
| Abstract | Collective epithelial migration is important throughout embryonic development. The underlying mechanisms are poorly understood but likely involve spatially localized activation of Rho GTPases. We previously reported that Rac1 is essential for generating the protrusive activity that drives the collective migration of anterior visceral endoderm (AVE) cells in the early mouse embryo. To identify potential regulators of Rac1, we first performed an RNAi screen of Rho family exchange factors (guanine nucleotide exchange factor [GEF]) in an in vitro collective epithelial migration assay and identified β-Pix. Genetic deletion of β-Pix in mice disrupts collective AVE migration, while high-resolution live imaging revealed that this is associated with randomly directed protrusive activity. We conclude that β-Pix controls the spatial localization of Rac1 activity to drive collective AVE migration at a critical stage in mouse development. |
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| MeSH term(s) | Animals ; Cell Movement/genetics ; Embryo, Mammalian ; Endoderm/cytology ; Epithelial Cells/cytology ; Gene Deletion ; Mice ; Mice, Knockout ; Neuropeptides/genetics ; Neuropeptides/metabolism ; Protein Transport/genetics ; Rho Guanine Nucleotide Exchange Factors/genetics ; Rho Guanine Nucleotide Exchange Factors/metabolism ; Viscera/cytology ; rac1 GTP-Binding Protein/genetics ; rac1 GTP-Binding Protein/metabolism | ||||||||||
| Chemical Substances | Neuropeptides ; Rac1 protein, mouse ; Rho Guanine Nucleotide Exchange Factors ; rac1 GTP-Binding Protein (EC 3.6.5.2) | ||||||||||
| Language | English | ||||||||||
| Publishing date | 2014-12-15 | ||||||||||
| Publishing country | United States | ||||||||||
| Document type | Journal Article ; Research Support, N.I.H., Extramural | ||||||||||
| ZDB-ID | 806684-x | ||||||||||
| ISSN | 1549-5477 ; 0890-9369 | ||||||||||
| ISSN (online) | 1549-5477 | ||||||||||
| ISSN | 0890-9369 | ||||||||||
| DOI | 10.1101/gad.251371.114 | ||||||||||
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| Database | MEDical Literature Analysis and Retrieval System OnLINE |
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